Dynamic changes in proprotein convertase 2 activity in cortical neurons after ischemia/reperfusion andoxygen-glucose deprivation

In this study, a rat model of transient focal cerebral ischemia was established by performing 100 minutes of middle cerebral artery occlusion, and an in vitro model of experimental oxygen-glucose deprivation using cultured rat cortical neurons was established. Proprotein convertase 2 activity gradua...

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Bibliographic Details
Published in:中国神经再生研究：英文版 2013, Vol.8 (1), p.83-89
Main Author: Shuqin Zhan An Zhou Chelsea Piper Tao Yang
Format: Article
Language:English
Subjects:
再灌注
动态变化
大鼠模型
皮层神经元
皮质神经元
缺血
脱氧核苷酸
蛋白
转化酶
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Summary:In this study, a rat model of transient focal cerebral ischemia was established by performing 100 minutes of middle cerebral artery occlusion, and an in vitro model of experimental oxygen-glucose deprivation using cultured rat cortical neurons was established. Proprotein convertase 2 activity gradually decreased in the ischemic cortex with increasing duration of reperfusion. In cultured rat cortical neurons, the number of terminal deoxynucleotidyl transferase-mediated 2＇-deoxyuridine 5＇-triphosphate-biotin nick end labeling-positive neurons significantly increased and proprotein convertase 2 activity also decreased gradually with increasing duration of oxygen-glucose deprivation. These experimental findings indicate that proprotein convertase 2 activity decreases in ischemic rat cortex after reperfusion, as well as in cultured rat cortical neurons after oxygen-glucose deprivation. These changes in enzyme activity may play an important pathological role in brain injury.
ISSN:1673-5374