Development of a Rapid Multi-residue Assay for Detecting β-Iactams Using Penicillin Binding Protein

Objective To develop a rapid multi-residue assay for detecting 16 demanded by the European Union （EU）. Methods A recombinant penicillin-binding protein （PBP） 2x＊ from Streptococcus pneumoniae R6 was expressed in vitro and six β-1actams were conjugated to HRP by four methods. A rapid multi-residue as...

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Published in:生物医学与环境科学：英文版 2013, Vol.26 (2), p.100-109
Main Author: ZENG Kun ZHANG Jing WANG Yang WANG Zhan Hui ZHANG Su Xia WU Chong Ming SHEN Jian Zhong
Format: Article
Language:English
Subjects:
多残留分析
多残留检测
头孢氨苄
快速检测方法
欧洲联盟
结合蛋白
肺炎链球菌
青霉素G
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Summary:Objective To develop a rapid multi-residue assay for detecting 16 demanded by the European Union （EU）. Methods A recombinant penicillin-binding protein （PBP） 2x＊ from Streptococcus pneumoniae R6 was expressed in vitro and six β-1actams were conjugated to HRP by four methods. A rapid multi-residue assay for β-1actams was established with PBP2x＊ and HRP-conjugate. Results PBP2x＊ was expressed and purified successfully and the ideal HRP-conjugate was identified. The multi-residue assay was developed. After optimization, penicillin G, ampicillin, amoxicillin, cloxacillin, dicloxacillin, oxacillin, nafcillin, cephalexin, ceftiofur, cefalonium, cefquinome, cefazolin, cefoperazone, cephacetrile, and cephapirin can be detected at levels below MRL in milk with simple pretreatment. Conclusion This assay developed can detect all 16 β-1actams demanded by the European Union （EU）. The whole procedure takes only 45 min and can detect 42 samples and the standards with duplicate analysis.
ISSN:0895-3988
2214-0190