Spindle and Chromosome Analysis of Non-Fertilized Oocytes from Conventional in vitro FertiliTation for Non-Male Factor Infertiliy： Significance of Rescue ICSI

Objective To determine whether rescue intracytoplasmic sperm injection （ICSI） is associated with improved outcomes for non-male factor infertility. Methods The changes of micro-structure, including meiotic spindle and chromosome distribution, sperm penetration, as well as oocyte activation were comp...

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Published in:	生殖与避孕：英文版 2011, Vol.22 (1), p.1-10
Main Author:	Xiao-lin LONG, Han-yan LIU Xin-jie CHEN Xiao-fang SUN
Format:	Article
Language:	eng
Subjects:	ICSI 主轴体外受精卵母细胞体外成熟卵胞浆内单精子注射救援染色体分析男性
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Summary:	Objective To determine whether rescue intracytoplasmic sperm injection （ICSI） is associated with improved outcomes for non-male factor infertility. Methods The changes of micro-structure, including meiotic spindle and chromosome distribution, sperm penetration, as well as oocyte activation were compared in IVF （in vitro fertilization）fertilization failure （1VF FF） patients, ICSI patients with non-fertilized oocytes （ICSI NF） and oocytes in vitro maturation （IVM）. Results A total of 164 unfertilized oocytes （93 oocytes in IVF and 71 oocytes in ICSI） and 56 IVM oocytes were available for this study. The abnormality of spindle and chromosomes was significantly higher in IVF FF group than that in ICSI NF group or IVM group （abnormal spindle rates were 80.6%, 64.8% and 57.1%, respectively; abnormal chromosome rates were 91.4%, 80.3% and 75.0%, respectively）. No sperm penetration after IVF and sperm expulsion after ICSI were 78.5% and 40.8%, respectively. Activation failure occurred in 16.1% of the IVF FF cases and 49.3% of ICSI NF oocytes. Conclusion Rescue ICSI of fertilization-failed oocytes fails to lead outcome improvement due to the internal defects of oocytes.
ISSN:	1001-7844