ANTIHYPERGLYCEMIC PROFILE OF ERINIDINE ISOLATED FROM HUNTERIA UMBELLATA SEED

Water decoction made from the seed of Hunteria umbellata is widely used in the traditional management of diabetes mellitus by Nigerian herbalists, particularly, in the southwest region of the country. Recently, a new bisindole alkaloid, erinidine, was isolated but its antihyperglycemic profile remai...

Full description

Saved in:
Bibliographic Details
Published in:African journal of traditional, complementary, and alternative medicines complementary, and alternative medicines, 2014-07, Vol.10 (2)
Main Authors: Adewale, Adeneye Adejuwon, Anthony, Crooks Peter, Zaineb, Fadhel-Albayati, Anne- Frances, Miller, Williams, Zito S, Olaide, Adeyemi Olufunmilayo, Oluwatoyin, Agbaje Esther
Format: Article
Language:
Subjects:
Alpha-glucosidase inhibition
Erinidine
Hunteria umbellata
Hyperglycemia
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Water decoction made from the seed of Hunteria umbellata is widely used in the traditional management of diabetes mellitus by Nigerian herbalists, particularly, in the southwest region of the country. Recently, a new bisindole alkaloid, erinidine, was isolated but its antihyperglycemic profile remains largely un-investigated scientifically. This forms the basis for the current study which is primarily designed at investigating the antihyperglycemic profile of erinidine and other fractions in both in vitro and in vivo models of diabetes mellitus. In the present study, erinidine was isolated and purified using the earlier described methods and its antihyperglycemic potentials tested in in vitro models such as dipeptidylpeptidase (IV), glycogen phosphorylase, HIT-T15 cell insulin secretion, glucose uptake activity, aldose reductase assays and α-glucosidase inhibition assay testings. In addition, 50 mg/kg of erinidine and that of other fractions were evaluated in in vivo models of normal and chemically-induced hyperglycemic rats. Results showed that erinidine was a light yellow, amorphous solid with UV (CHCl3) λmax 256 nm, HRESIMS m/z 382.1881 [(M+H)+] (calculated for C22H26N4O2, 382.1876) and melting point of 230 ºC. The in vitro study showed the antihyperglycemic action of erinidine to be weakly mediated via α-glucosidase inhibition mechanism as the results for other in vitro tests such as dipeptidylpeptidase (IV), glycogen phosphorylase, HIT-T15 cell insulin secretion, glucose uptake activity and aldose reductase assays were all negative. However, the in vivo results showed 50 mg/kg erinidine given per os to normal and alloxan-induced hyperglycemic rats to significantly (p
ISSN:0189-6016
2505-0044