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In Vitro Anticholinesterase and Inhibitory Effects of the Aqueous Extract of Combretum molle (Combretaceae) Leaf on Rabbit Breathing
Purpose: In previous studies, the aqueous extract of Combretum molle was shown to inhibit disturbances of breathing induced by acetylcholine. The aim of this study was to elucidate the mechanism of this inhibition. Methods: The aqueous extract of Combretum molle and an anti-asthmatic, salbutamol (re...
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Published in: | Tropical journal of pharmaceutical research 2011-05, Vol.9 (5) |
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Main Authors: | , , , , , , , |
Format: | Article |
Language: | |
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Online Access: | Get full text |
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Summary: | Purpose: In previous studies, the aqueous extract of Combretum molle
was shown to inhibit disturbances of breathing induced by
acetylcholine. The aim of this study was to elucidate the mechanism of
this inhibition. Methods: The aqueous extract of Combretum molle and an
anti-asthmatic, salbutamol (reference), were tested at varying
concentrations for their inhibitory effects on breathing.
Acetylcholinesterase (AChE) was extracted from rabbit duodenum and its
activity determined by Ellman's assay using acethylthiocholine
iodide (ACTH) as substrate. The rate of hydrolysis of
acetylcholinesterase was spectrophotometrically monitored at 412 nm.
Results: The results indicate that in the presence of C. molle extract,
a considerable reduction in the variation of breathing amplitudes
occurred. Furthermore, the extract probably contains anti-AChE
substances. C. molle exerted non-competitive inhibition of
acetylcholinesterase with Michaelis-Menten constant (KM) of 192 μM
and velocity at maximal concentration of substrate (Vmax) of 4444
μM/min. Conclusion: These results support the use of C. molle leaf
in the Pharmacopoeia of Ivory Coast as an anti-asthmatic, in view of
its inhibitory effect on breathing disturbances. |
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ISSN: | 1596-5996 1596-5996 |