Acute molecular responses to concurrent resistance and high-intensity interval exercise in untrained skeletal muscle

Concurrent training involving resistance and endurance exercise may augment the benefits of single‐mode training for the purpose of improving health. However, muscle adaptations, associated with resistance exercise, may be blunted by a subsequent bout of endurance exercise, via molecular interferenc...

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Main Authors:	Jamie Pugh, Steve Faulkner, Andy Jackson, James King, Myra A. Nimmo
Format:	Default Article
Published:	2015
Subjects:	Animal physiology - biophysics Animal physiology - cell Animal physiology - systems Other health sciences not elsewhere classified Acute responses Cellular signaling interference Concurrent exercise High intensity interval training Human skeletal muscle Physiology Medical and Health Sciences not elsewhere classified
Online Access:	https://hdl.handle.net/2134/18121
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id	rr-article-9618278
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spelling	rr-article-96182782015-01-01T00:00:00Z Acute molecular responses to concurrent resistance and high-intensity interval exercise in untrained skeletal muscle Jamie Pugh (1248333) Steve Faulkner (1249419) Andy Jackson (1254042) James King (1252200) Myra A. Nimmo (7238924) Animal physiology - biophysics Animal physiology - cell Animal physiology - systems Other health sciences not elsewhere classified Acute responses Cellular signaling interference Concurrent exercise High intensity interval training Human skeletal muscle Physiology Medical and Health Sciences not elsewhere classified Concurrent training involving resistance and endurance exercise may augment the benefits of single‐mode training for the purpose of improving health. However, muscle adaptations, associated with resistance exercise, may be blunted by a subsequent bout of endurance exercise, via molecular interference. High‐intensity interval training (HIIT), generating similar adaptations to endurance exercise, may offer an alternative exercise mode to traditional endurance exercise. This study examined the influence of an acute HIIT session on the molecular responses following resistance exercise in untrained skeletal muscle. Ten male participants performed resistance exercise (4 × 8 leg extensions, 70% 1RM, (RE)) or RE followed by HIIT (10 × 1 min at 90% HRmax, (RE+HIIT)). Muscle biopsies were collected from the vastus lateralis before, 2 and 6 h post‐RE to determine intramuscular protein phosphorylation and mRNA responses. Phosphorylation of Akt (Ser473) decreased at 6 h in both trials (P < 0.05). Phosphorylation of mTOR (Ser2448) was higher in RE+HIIT (P < 0.05). All PGC‐1α mRNA variants increased at 2 h in RE+HIIT with PGC‐1α and PGC‐1α‐ex1b remaining elevated at 6 h, whereas RE‐induced increases at 2 and 6 h for PGC‐1α‐ex1b only (P < 0.05). Myostatin expression decreased at 2 and 6 h in both trials (P < 0.05). MuRF‐1 was elevated in RE+HIIT versus RE at 2 and 6 h (P < 0.05). Atrogin‐1 was lower at 2 h, with FOXO3A downregulated at 6 h (P < 0.05). These data do not support the existence of an acute interference effect on protein signaling and mRNA expression, and suggest that HIIT may be an alternative to endurance exercise when performed after resistance exercise in the same training session to optimize adaptations. 2015-01-01T00:00:00Z Text Journal contribution 2134/18121 https://figshare.com/articles/journal_contribution/Acute_molecular_responses_to_concurrent_resistance_and_high-intensity_interval_exercise_in_untrained_skeletal_muscle/9618278 CC BY 3.0
institution	Loughborough University
collection	Figshare
topic	Animal physiology - biophysics Animal physiology - cell Animal physiology - systems Other health sciences not elsewhere classified Acute responses Cellular signaling interference Concurrent exercise High intensity interval training Human skeletal muscle Physiology Medical and Health Sciences not elsewhere classified
spellingShingle	Animal physiology - biophysics Animal physiology - cell Animal physiology - systems Other health sciences not elsewhere classified Acute responses Cellular signaling interference Concurrent exercise High intensity interval training Human skeletal muscle Physiology Medical and Health Sciences not elsewhere classified Jamie Pugh Steve Faulkner Andy Jackson James King Myra A. Nimmo Acute molecular responses to concurrent resistance and high-intensity interval exercise in untrained skeletal muscle
description	Concurrent training involving resistance and endurance exercise may augment the benefits of single‐mode training for the purpose of improving health. However, muscle adaptations, associated with resistance exercise, may be blunted by a subsequent bout of endurance exercise, via molecular interference. High‐intensity interval training (HIIT), generating similar adaptations to endurance exercise, may offer an alternative exercise mode to traditional endurance exercise. This study examined the influence of an acute HIIT session on the molecular responses following resistance exercise in untrained skeletal muscle. Ten male participants performed resistance exercise (4 × 8 leg extensions, 70% 1RM, (RE)) or RE followed by HIIT (10 × 1 min at 90% HRmax, (RE+HIIT)). Muscle biopsies were collected from the vastus lateralis before, 2 and 6 h post‐RE to determine intramuscular protein phosphorylation and mRNA responses. Phosphorylation of Akt (Ser473) decreased at 6 h in both trials (P < 0.05). Phosphorylation of mTOR (Ser2448) was higher in RE+HIIT (P < 0.05). All PGC‐1α mRNA variants increased at 2 h in RE+HIIT with PGC‐1α and PGC‐1α‐ex1b remaining elevated at 6 h, whereas RE‐induced increases at 2 and 6 h for PGC‐1α‐ex1b only (P < 0.05). Myostatin expression decreased at 2 and 6 h in both trials (P < 0.05). MuRF‐1 was elevated in RE+HIIT versus RE at 2 and 6 h (P < 0.05). Atrogin‐1 was lower at 2 h, with FOXO3A downregulated at 6 h (P < 0.05). These data do not support the existence of an acute interference effect on protein signaling and mRNA expression, and suggest that HIIT may be an alternative to endurance exercise when performed after resistance exercise in the same training session to optimize adaptations.
format	Default Article
author	Jamie Pugh Steve Faulkner Andy Jackson James King Myra A. Nimmo
author_facet	Jamie Pugh Steve Faulkner Andy Jackson James King Myra A. Nimmo
author_sort	Jamie Pugh (1248333)
title	Acute molecular responses to concurrent resistance and high-intensity interval exercise in untrained skeletal muscle
title_short	Acute molecular responses to concurrent resistance and high-intensity interval exercise in untrained skeletal muscle
title_full	Acute molecular responses to concurrent resistance and high-intensity interval exercise in untrained skeletal muscle
title_fullStr	Acute molecular responses to concurrent resistance and high-intensity interval exercise in untrained skeletal muscle
title_full_unstemmed	Acute molecular responses to concurrent resistance and high-intensity interval exercise in untrained skeletal muscle
title_sort	acute molecular responses to concurrent resistance and high-intensity interval exercise in untrained skeletal muscle
publishDate	2015
url	https://hdl.handle.net/2134/18121
_version_	1797737373004464128

Acute molecular responses to concurrent resistance and high-intensity interval exercise in untrained skeletal muscle

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