Early apoptosis of porcine alveolar macrophages limits avian influenza virus replication and pro-inflammatory dysregulation

Pigs are evidently more resistant to avian than swine influenza A viruses, mediated in part through frontline epithelial cells and alveolar macrophages (AM). Although porcine AM (PAM) are crucial in influenza virus control, their mode of control is unclear. To gain insight into the possible role of...

Full description

Saved in:

Bibliographic Details
Main Authors:	Pengxiang Chang, Suresh V. Kuchipudi, Kenneth H. Mellits, Sujith Sebastian, Joe James, Jinhua Liu, Holly Shelton, Kin-Chow Chang
Format:	Default Article
Published:	2015
Subjects:	Mechanical engineering not elsewhere classified Viruses Avian flu H5N1 Pigs PB1-F2 Disease Infection Resistance Mechanical Engineering not elsewhere classified
Online Access:	https://hdl.handle.net/2134/20615
Tags:	Add Tag No Tags, Be the first to tag this record!

id	rr-article-9567791
record_format	Figshare
spelling	rr-article-95677912015-01-01T00:00:00Z Early apoptosis of porcine alveolar macrophages limits avian influenza virus replication and pro-inflammatory dysregulation Pengxiang Chang (4099426) Suresh V. Kuchipudi (7211204) Kenneth H. Mellits (7214228) Sujith Sebastian (1247778) Joe James (7214231) Jinhua Liu (189844) Holly Shelton (202275) Kin-Chow Chang (64619) Mechanical engineering not elsewhere classified Viruses Avian flu H5N1 Pigs PB1-F2 Disease Infection Resistance Mechanical Engineering not elsewhere classified Pigs are evidently more resistant to avian than swine influenza A viruses, mediated in part through frontline epithelial cells and alveolar macrophages (AM). Although porcine AM (PAM) are crucial in influenza virus control, their mode of control is unclear. To gain insight into the possible role of PAM in the mediation of avian influenza virus resistance, we compared the host effects and replication of two avian (H2N3 and H6N1) and three mammalian (swine H1N1, human H1N1 and pandemic H1N1) influenza viruses in PAM. We found that PAM were readily susceptible to initial infection with all five avian and mammalian influenza viruses but only avian viruses caused early and extensive apoptosis (by 6 h of infection) resulting in reduced virus progeny and moderated pro-inflammation. Full length viral PB1-F2 present only in avian influenza viruses is a virulence factor that targets AM for mitochondrial-associated apoptotic cell death. With the use of reverse genetics on an avian H5N1 virus, we found that full length PB1-F2 contributed to increased apoptosis and pro-inflammation but not to reduced virus replication. Taken together, we propose that early apoptosis of PAM limits the spread of avian influenza viruses and that PB1-F2 could play a contributory role in the process. 2015-01-01T00:00:00Z Text Journal contribution 2134/20615 https://figshare.com/articles/journal_contribution/Early_apoptosis_of_porcine_alveolar_macrophages_limits_avian_influenza_virus_replication_and_pro-inflammatory_dysregulation/9567791 CC BY 4.0
institution	Loughborough University
collection	Figshare
topic	Mechanical engineering not elsewhere classified Viruses Avian flu H5N1 Pigs PB1-F2 Disease Infection Resistance Mechanical Engineering not elsewhere classified
spellingShingle	Mechanical engineering not elsewhere classified Viruses Avian flu H5N1 Pigs PB1-F2 Disease Infection Resistance Mechanical Engineering not elsewhere classified Pengxiang Chang Suresh V. Kuchipudi Kenneth H. Mellits Sujith Sebastian Joe James Jinhua Liu Holly Shelton Kin-Chow Chang Early apoptosis of porcine alveolar macrophages limits avian influenza virus replication and pro-inflammatory dysregulation
description	Pigs are evidently more resistant to avian than swine influenza A viruses, mediated in part through frontline epithelial cells and alveolar macrophages (AM). Although porcine AM (PAM) are crucial in influenza virus control, their mode of control is unclear. To gain insight into the possible role of PAM in the mediation of avian influenza virus resistance, we compared the host effects and replication of two avian (H2N3 and H6N1) and three mammalian (swine H1N1, human H1N1 and pandemic H1N1) influenza viruses in PAM. We found that PAM were readily susceptible to initial infection with all five avian and mammalian influenza viruses but only avian viruses caused early and extensive apoptosis (by 6 h of infection) resulting in reduced virus progeny and moderated pro-inflammation. Full length viral PB1-F2 present only in avian influenza viruses is a virulence factor that targets AM for mitochondrial-associated apoptotic cell death. With the use of reverse genetics on an avian H5N1 virus, we found that full length PB1-F2 contributed to increased apoptosis and pro-inflammation but not to reduced virus replication. Taken together, we propose that early apoptosis of PAM limits the spread of avian influenza viruses and that PB1-F2 could play a contributory role in the process.
format	Default Article
author	Pengxiang Chang Suresh V. Kuchipudi Kenneth H. Mellits Sujith Sebastian Joe James Jinhua Liu Holly Shelton Kin-Chow Chang
author_facet	Pengxiang Chang Suresh V. Kuchipudi Kenneth H. Mellits Sujith Sebastian Joe James Jinhua Liu Holly Shelton Kin-Chow Chang
author_sort	Pengxiang Chang (4099426)
title	Early apoptosis of porcine alveolar macrophages limits avian influenza virus replication and pro-inflammatory dysregulation
title_short	Early apoptosis of porcine alveolar macrophages limits avian influenza virus replication and pro-inflammatory dysregulation
title_full	Early apoptosis of porcine alveolar macrophages limits avian influenza virus replication and pro-inflammatory dysregulation
title_fullStr	Early apoptosis of porcine alveolar macrophages limits avian influenza virus replication and pro-inflammatory dysregulation
title_full_unstemmed	Early apoptosis of porcine alveolar macrophages limits avian influenza virus replication and pro-inflammatory dysregulation
title_sort	early apoptosis of porcine alveolar macrophages limits avian influenza virus replication and pro-inflammatory dysregulation
publishDate	2015
url	https://hdl.handle.net/2134/20615
_version_	1799639504879878144

Early apoptosis of porcine alveolar macrophages limits avian influenza virus replication and pro-inflammatory dysregulation

Similar Items