Generation of human induced pluripotent stem cells using non-synthetic mRNA.

Generation of human induced pluripotent stem cells using non-synthetic mRNA.

Here we describe some of the crucial steps to generate induced pluripotent stem cells (iPSCs) using mRNA transfection. Our approach uses a V. virus-derived capping enzyme instead of a cap-analog, ensuring 100% proper cap orientation for in vitro transcribed mRNA. V. virus' 2'-O-Methyltrans...

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Bibliographic Details
Main Authors: Leili Rohani, Claire Fabian, H. Holland, Yahaira Naaldijk, R. Dressel, H. Loffler-Wirth, Hans Binder, Antje Arnold, Alexandra Stolzing
Format: Default Article
Published: 2016
Subjects:
Mechanical engineering not elsewhere classified
Induced pluripotent stem cell
Reprogramming
mRNA
Mechanical Engineering not elsewhere classified
Online Access:https://hdl.handle.net/2134/20950
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https://hdl.handle.net/2134/20950

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