Anticalcification potential of heparin on hydroxyapatite seeds using a constant composition in vitro model

Calcification is among the principal causes of biological heart valve substitute failure. Glycosaminoglcans (GAGs) are negatively charged molecules, possessing anticoagulation and anti-inflammatory activity that make them a potential solution against calcification. In the present work, the anticalci...

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Main Authors:	Adel Badria, Petros Koutsoukos, Cristian D'Alessandro, Sotiris Korossis, Dimosthenis Mavrilas
Format:	Default Article
Published:	2018
Subjects:	Mechanical engineering not elsewhere classified untagged Mechanical Engineering not elsewhere classified
Online Access:	https://hdl.handle.net/2134/37010
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id	rr-article-9548519
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spelling	rr-article-95485192018-07-11T00:00:00Z Anticalcification potential of heparin on hydroxyapatite seeds using a constant composition in vitro model Adel Badria (7205129) Petros Koutsoukos (2714614) Cristian D'Alessandro (7206941) Sotiris Korossis (5214488) Dimosthenis Mavrilas (7205132) Mechanical engineering not elsewhere classified untagged Mechanical Engineering not elsewhere classified Calcification is among the principal causes of biological heart valve substitute failure. Glycosaminoglcans (GAGs) are negatively charged molecules, possessing anticoagulation and anti-inflammatory activity that make them a potential solution against calcification. In the present work, the anticalcification potential of heparin was investigated under constant supersaturation conditions with respect to hydroxyapatite (Ca5(PO4)3OH; HAP). Heparin concentration in the supersaturated solutions was in the range between 0.25 and 3 ppm, at pH 7.40 and 37 °C. Heparin showed inhibitory activity, which was attributed to adsorption at the active crystal growth centres. Heparin concentration as low as ca. 50 μM, reduced the rate of HAP crystal growth by more than 80%, while further increase (up to 200 μM) failed to completely inhibit the process beyond 90%. Heparin uptake studies at equilibrium conditions and analysis of the kinetics data in the presence of heparin, strongly suggest that the inhibition is due to the adsorption of heparin onto the HAP crystals, which resulted in the poisoning of the active growth sites. 2018-07-11T00:00:00Z Text Journal contribution 2134/37010 https://figshare.com/articles/journal_contribution/Anticalcification_potential_of_heparin_on_hydroxyapatite_seeds_using_a_constant_composition_in_vitro_model/9548519 CC BY-NC-ND 4.0
institution	Loughborough University
collection	Figshare
topic	Mechanical engineering not elsewhere classified untagged Mechanical Engineering not elsewhere classified
spellingShingle	Mechanical engineering not elsewhere classified untagged Mechanical Engineering not elsewhere classified Adel Badria Petros Koutsoukos Cristian D'Alessandro Sotiris Korossis Dimosthenis Mavrilas Anticalcification potential of heparin on hydroxyapatite seeds using a constant composition in vitro model
description	Calcification is among the principal causes of biological heart valve substitute failure. Glycosaminoglcans (GAGs) are negatively charged molecules, possessing anticoagulation and anti-inflammatory activity that make them a potential solution against calcification. In the present work, the anticalcification potential of heparin was investigated under constant supersaturation conditions with respect to hydroxyapatite (Ca5(PO4)3OH; HAP). Heparin concentration in the supersaturated solutions was in the range between 0.25 and 3 ppm, at pH 7.40 and 37 °C. Heparin showed inhibitory activity, which was attributed to adsorption at the active crystal growth centres. Heparin concentration as low as ca. 50 μM, reduced the rate of HAP crystal growth by more than 80%, while further increase (up to 200 μM) failed to completely inhibit the process beyond 90%. Heparin uptake studies at equilibrium conditions and analysis of the kinetics data in the presence of heparin, strongly suggest that the inhibition is due to the adsorption of heparin onto the HAP crystals, which resulted in the poisoning of the active growth sites.
format	Default Article
author	Adel Badria Petros Koutsoukos Cristian D'Alessandro Sotiris Korossis Dimosthenis Mavrilas
author_facet	Adel Badria Petros Koutsoukos Cristian D'Alessandro Sotiris Korossis Dimosthenis Mavrilas
author_sort	Adel Badria (7205129)
title	Anticalcification potential of heparin on hydroxyapatite seeds using a constant composition in vitro model
title_short	Anticalcification potential of heparin on hydroxyapatite seeds using a constant composition in vitro model
title_full	Anticalcification potential of heparin on hydroxyapatite seeds using a constant composition in vitro model
title_fullStr	Anticalcification potential of heparin on hydroxyapatite seeds using a constant composition in vitro model
title_full_unstemmed	Anticalcification potential of heparin on hydroxyapatite seeds using a constant composition in vitro model
title_sort	anticalcification potential of heparin on hydroxyapatite seeds using a constant composition in vitro model
publishDate	2018
url	https://hdl.handle.net/2134/37010
_version_	1797732827536556032

Anticalcification potential of heparin on hydroxyapatite seeds using a constant composition in vitro model

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