Antioxidants effectively prevent oxidation-induced protein damage in OLN 93 cells

Oxidative stress is supposed to play an important role in demyelinating diseases. Oligodendrocytes are the myelin-forming cells in the brain and are highly susceptible to oxidative stress due to their low antioxidative defense systems and high metabolic rate. In the present work, we tested the respo...

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Main Authors: Andrea Ernst, Alexandra Stolzing, Grit Sandig, Tilman Grune
Format: Default Article
Published: 2004
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Online Access:https://hdl.handle.net/2134/16902
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spelling rr-article-95477992004-01-01T00:00:00Z Antioxidants effectively prevent oxidation-induced protein damage in OLN 93 cells Andrea Ernst (7206515) Alexandra Stolzing (1251348) Grit Sandig (7206518) Tilman Grune (1296930) Mechanical engineering not elsewhere classified Antioxidants Free radicals Oligodendrocytes OLN93 cells Protein oxidation Mechanical Engineering not elsewhere classified Oxidative stress is supposed to play an important role in demyelinating diseases. Oligodendrocytes are the myelin-forming cells in the brain and are highly susceptible to oxidative stress due to their low antioxidative defense systems and high metabolic rate. In the present work, we tested the response of the oligodendrocyte cell line OLN 93 to oxidative stress. OLN 93 cell cultures are characterized by a loss of cell viability after oxidation. This loss of cell viability is accompanied by an increase in protein oxidation and consequently an elevated overall proteolysis. To minimize the oxidative damage, we tested the effects of the antioxidants α-lipoic acid and coenzyme Q10. Both compounds were able to elevate cell viability and to decrease intracellular protein turnover and oxidant induced protein oxidation. Therefore, we concluded that the excessive oxidative damage of oligodendrocytes and their protein pool can be prevented by the usage of antioxidants. 2004-01-01T00:00:00Z Text Journal contribution 2134/16902 https://figshare.com/articles/journal_contribution/Antioxidants_effectively_prevent_oxidation-induced_protein_damage_in_OLN_93_cells/9547799 CC BY-NC-ND 4.0
institution Loughborough University
collection Figshare
topic Mechanical engineering not elsewhere classified
Antioxidants
Free radicals
Oligodendrocytes
OLN93 cells
Protein oxidation
Mechanical Engineering not elsewhere classified
spellingShingle Mechanical engineering not elsewhere classified
Antioxidants
Free radicals
Oligodendrocytes
OLN93 cells
Protein oxidation
Mechanical Engineering not elsewhere classified
Andrea Ernst
Alexandra Stolzing
Grit Sandig
Tilman Grune
Antioxidants effectively prevent oxidation-induced protein damage in OLN 93 cells
description Oxidative stress is supposed to play an important role in demyelinating diseases. Oligodendrocytes are the myelin-forming cells in the brain and are highly susceptible to oxidative stress due to their low antioxidative defense systems and high metabolic rate. In the present work, we tested the response of the oligodendrocyte cell line OLN 93 to oxidative stress. OLN 93 cell cultures are characterized by a loss of cell viability after oxidation. This loss of cell viability is accompanied by an increase in protein oxidation and consequently an elevated overall proteolysis. To minimize the oxidative damage, we tested the effects of the antioxidants α-lipoic acid and coenzyme Q10. Both compounds were able to elevate cell viability and to decrease intracellular protein turnover and oxidant induced protein oxidation. Therefore, we concluded that the excessive oxidative damage of oligodendrocytes and their protein pool can be prevented by the usage of antioxidants.
format Default
Article
author Andrea Ernst
Alexandra Stolzing
Grit Sandig
Tilman Grune
author_facet Andrea Ernst
Alexandra Stolzing
Grit Sandig
Tilman Grune
author_sort Andrea Ernst (7206515)
title Antioxidants effectively prevent oxidation-induced protein damage in OLN 93 cells
title_short Antioxidants effectively prevent oxidation-induced protein damage in OLN 93 cells
title_full Antioxidants effectively prevent oxidation-induced protein damage in OLN 93 cells
title_fullStr Antioxidants effectively prevent oxidation-induced protein damage in OLN 93 cells
title_full_unstemmed Antioxidants effectively prevent oxidation-induced protein damage in OLN 93 cells
title_sort antioxidants effectively prevent oxidation-induced protein damage in oln 93 cells
publishDate 2004
url https://hdl.handle.net/2134/16902
_version_ 1794842050993061888