Antioxidants effectively prevent oxidation-induced protein damage in OLN 93 cells
Oxidative stress is supposed to play an important role in demyelinating diseases. Oligodendrocytes are the myelin-forming cells in the brain and are highly susceptible to oxidative stress due to their low antioxidative defense systems and high metabolic rate. In the present work, we tested the respo...
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rr-article-95477992004-01-01T00:00:00Z Antioxidants effectively prevent oxidation-induced protein damage in OLN 93 cells Andrea Ernst (7206515) Alexandra Stolzing (1251348) Grit Sandig (7206518) Tilman Grune (1296930) Mechanical engineering not elsewhere classified Antioxidants Free radicals Oligodendrocytes OLN93 cells Protein oxidation Mechanical Engineering not elsewhere classified Oxidative stress is supposed to play an important role in demyelinating diseases. Oligodendrocytes are the myelin-forming cells in the brain and are highly susceptible to oxidative stress due to their low antioxidative defense systems and high metabolic rate. In the present work, we tested the response of the oligodendrocyte cell line OLN 93 to oxidative stress. OLN 93 cell cultures are characterized by a loss of cell viability after oxidation. This loss of cell viability is accompanied by an increase in protein oxidation and consequently an elevated overall proteolysis. To minimize the oxidative damage, we tested the effects of the antioxidants α-lipoic acid and coenzyme Q10. Both compounds were able to elevate cell viability and to decrease intracellular protein turnover and oxidant induced protein oxidation. Therefore, we concluded that the excessive oxidative damage of oligodendrocytes and their protein pool can be prevented by the usage of antioxidants. 2004-01-01T00:00:00Z Text Journal contribution 2134/16902 https://figshare.com/articles/journal_contribution/Antioxidants_effectively_prevent_oxidation-induced_protein_damage_in_OLN_93_cells/9547799 CC BY-NC-ND 4.0 |
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Mechanical engineering not elsewhere classified Antioxidants Free radicals Oligodendrocytes OLN93 cells Protein oxidation Mechanical Engineering not elsewhere classified |
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Mechanical engineering not elsewhere classified Antioxidants Free radicals Oligodendrocytes OLN93 cells Protein oxidation Mechanical Engineering not elsewhere classified Andrea Ernst Alexandra Stolzing Grit Sandig Tilman Grune Antioxidants effectively prevent oxidation-induced protein damage in OLN 93 cells |
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Oxidative stress is supposed to play an important role in demyelinating diseases. Oligodendrocytes are the myelin-forming cells in the brain and are highly susceptible to oxidative stress due to their low antioxidative defense systems and high metabolic rate. In the present work, we tested the response of the oligodendrocyte cell line OLN 93 to oxidative stress. OLN 93 cell cultures are characterized by a loss of cell viability after oxidation. This loss of cell viability is accompanied by an increase in protein oxidation and consequently an elevated overall proteolysis. To minimize the oxidative damage, we tested the effects of the antioxidants α-lipoic acid and coenzyme Q10. Both compounds were able to elevate cell viability and to decrease intracellular protein turnover and oxidant induced protein oxidation. Therefore, we concluded that the excessive oxidative damage of oligodendrocytes and their protein pool can be prevented by the usage of antioxidants. |
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Default Article |
author |
Andrea Ernst Alexandra Stolzing Grit Sandig Tilman Grune |
author_facet |
Andrea Ernst Alexandra Stolzing Grit Sandig Tilman Grune |
author_sort |
Andrea Ernst (7206515) |
title |
Antioxidants effectively prevent oxidation-induced protein damage in OLN 93 cells |
title_short |
Antioxidants effectively prevent oxidation-induced protein damage in OLN 93 cells |
title_full |
Antioxidants effectively prevent oxidation-induced protein damage in OLN 93 cells |
title_fullStr |
Antioxidants effectively prevent oxidation-induced protein damage in OLN 93 cells |
title_full_unstemmed |
Antioxidants effectively prevent oxidation-induced protein damage in OLN 93 cells |
title_sort |
antioxidants effectively prevent oxidation-induced protein damage in oln 93 cells |
publishDate |
2004 |
url |
https://hdl.handle.net/2134/16902 |
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1794842050993061888 |