Effect of reduced culture temperature on antioxidant defences of mesenchymal stem cells

Mesenchymal stem cells (MSC) promise to be valuable therapeutic tools but, due to their low numbers, require considerable in vitro expansion before use. This leads to in vitro aging, the accumulation of intracellular oxidative damage, and subsequently a decreased potential for proliferation and diff...

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Main Authors:	Alexandra Stolzing, Andrew Scutt
Format:	Default Article
Published:	2006
Subjects:	Mechanical engineering not elsewhere classified Antioxidative defence Cold shock Differentiation Heat shock protein Mesenchymal stem cells Reactive oxygen species Mechanical Engineering not elsewhere classified
Online Access:	https://hdl.handle.net/2134/16868
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id	rr-article-9546497
record_format	Figshare
spelling	rr-article-95464972006-01-01T00:00:00Z Effect of reduced culture temperature on antioxidant defences of mesenchymal stem cells Alexandra Stolzing (1251348) Andrew Scutt (7204796) Mechanical engineering not elsewhere classified Antioxidative defence Cold shock Differentiation Heat shock protein Mesenchymal stem cells Reactive oxygen species Mechanical Engineering not elsewhere classified Mesenchymal stem cells (MSC) promise to be valuable therapeutic tools but, due to their low numbers, require considerable in vitro expansion before use. This leads to in vitro aging, the accumulation of intracellular oxidative damage, and subsequently a decreased potential for proliferation and differentiation. Optimised culture conditions might help to reduce oxidative damage in MSC in vitro, and therefore, as reduced temperature is known to reduce oxidative stress in other somatic cells, we have investigated the effect of reduced temperature on rat MSC viability, differentiation, and oxidative damage. Temperature reduction did not affect MSC viability but increased differentiation and reduced apoptosis. Oxidative-damage-related indices were improved; reactive oxide species, nitric oxide, thiobarbituric acid reactive substances, carbonyl, and lipofuscin levels were reduced and glutathione peroxidase and superoxide dimutase levels increased. Levels of antiapoptotic heat shock proteins (HSP-27, -70, and -90) were raised and levels of the proapoptotic HSP-60 reduced. These data demonstrate that culturing MSC at reduced temperature decreases the accumulation of oxidative damage and therefore would probably improve long-term viability and successful engraftment of MSC used for tissue engineering or cell therapeutic purposes. © 2006. 2006-01-01T00:00:00Z Text Journal contribution 2134/16868 https://figshare.com/articles/journal_contribution/Effect_of_reduced_culture_temperature_on_antioxidant_defences_of_mesenchymal_stem_cells/9546497 CC BY-NC-ND 4.0
institution	Loughborough University
collection	Figshare
topic	Mechanical engineering not elsewhere classified Antioxidative defence Cold shock Differentiation Heat shock protein Mesenchymal stem cells Reactive oxygen species Mechanical Engineering not elsewhere classified
spellingShingle	Mechanical engineering not elsewhere classified Antioxidative defence Cold shock Differentiation Heat shock protein Mesenchymal stem cells Reactive oxygen species Mechanical Engineering not elsewhere classified Alexandra Stolzing Andrew Scutt Effect of reduced culture temperature on antioxidant defences of mesenchymal stem cells
description	Mesenchymal stem cells (MSC) promise to be valuable therapeutic tools but, due to their low numbers, require considerable in vitro expansion before use. This leads to in vitro aging, the accumulation of intracellular oxidative damage, and subsequently a decreased potential for proliferation and differentiation. Optimised culture conditions might help to reduce oxidative damage in MSC in vitro, and therefore, as reduced temperature is known to reduce oxidative stress in other somatic cells, we have investigated the effect of reduced temperature on rat MSC viability, differentiation, and oxidative damage. Temperature reduction did not affect MSC viability but increased differentiation and reduced apoptosis. Oxidative-damage-related indices were improved; reactive oxide species, nitric oxide, thiobarbituric acid reactive substances, carbonyl, and lipofuscin levels were reduced and glutathione peroxidase and superoxide dimutase levels increased. Levels of antiapoptotic heat shock proteins (HSP-27, -70, and -90) were raised and levels of the proapoptotic HSP-60 reduced. These data demonstrate that culturing MSC at reduced temperature decreases the accumulation of oxidative damage and therefore would probably improve long-term viability and successful engraftment of MSC used for tissue engineering or cell therapeutic purposes. © 2006.
format	Default Article
author	Alexandra Stolzing Andrew Scutt
author_facet	Alexandra Stolzing Andrew Scutt
author_sort	Alexandra Stolzing (1251348)
title	Effect of reduced culture temperature on antioxidant defences of mesenchymal stem cells
title_short	Effect of reduced culture temperature on antioxidant defences of mesenchymal stem cells
title_full	Effect of reduced culture temperature on antioxidant defences of mesenchymal stem cells
title_fullStr	Effect of reduced culture temperature on antioxidant defences of mesenchymal stem cells
title_full_unstemmed	Effect of reduced culture temperature on antioxidant defences of mesenchymal stem cells
title_sort	effect of reduced culture temperature on antioxidant defences of mesenchymal stem cells
publishDate	2006
url	https://hdl.handle.net/2134/16868
_version_	1796740752555900928

Effect of reduced culture temperature on antioxidant defences of mesenchymal stem cells

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