Exploitation of GFP fusion proteins and stress avoidance as a generic strategy for the production of high quality recombinant proteins

Exploitation of GFP fusion proteins and stress avoidance as a generic strategy for the production of high quality recombinant proteins

A C-terminal green fluorescent protein (GFP) fusion to a model target protein, Escherichia coli CheY, was exploited both as a reporter of the accumulation of soluble recombinant protein, and to develop a generic approach to optimize protein yields. The rapid accumulation of CheY∷GFP expressed from a...

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Bibliographic Details
Main Authors: Yanina Sevastsyanovich, Sara Alfasi, Tim Overton, Richard Hall, Jo Jones, Christopher Hewitt, Jeff Cole
Format: Default Preprint
Published: 2009
Subjects:
Chemical engineering not elsewhere classified
Recombinant protein production
Inclusion bodies
Gonococcal 1 cytochrome c2
Heat shock
General stress response
Green fluorescent protein
Flow cytometry
Chemical Engineering not elsewhere classified
Online Access:https://hdl.handle.net/2134/5759
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https://hdl.handle.net/2134/5759

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