Enhanced co-culture and enrichment of human natural killer cells for the selective clearance of senescent cells

In the context of aging and age-associated diseases, Natural Killer (NK) cells have been revealed as a key cell type responsible for the immune clearance of senescent cells. Subsequently, NK cell-based therapies have emerged as promising alternatives to drug-based therapeutic interventions for the p...

Full description

Saved in:

Bibliographic Details
Main Authors:	Kristie Kim, Tesfahun Dessale Admasu, Alexandra Stolzing, Amit Sharma
Format:	Default Article
Published:	2022
Subjects:	Animal physiology - biophysics Animal physiology - cell Animal physiology - systems aging senescence natural killer cells NKCC immune surveillance Physiology
Online Access:	https://hdl.handle.net/2134/19233747.v1
Tags:	Add Tag No Tags, Be the first to tag this record!

id	rr-article-19233747
record_format	Figshare
spelling	rr-article-192337472022-03-04T00:00:00Z Enhanced co-culture and enrichment of human natural killer cells for the selective clearance of senescent cells Kristie Kim (12149775) Tesfahun Dessale Admasu (11180595) Alexandra Stolzing (1251348) Amit Sharma (46604) Animal physiology - biophysics Animal physiology - cell Animal physiology - systems aging senescence natural killer cells NKCC immune surveillance Physiology In the context of aging and age-associated diseases, Natural Killer (NK) cells have been revealed as a key cell type responsible for the immune clearance of senescent cells. Subsequently, NK cell-based therapies have emerged as promising alternatives to drug-based therapeutic interventions for the prevention and treatment of age-related disease and debility. Given the promise of NK cell-mediated immunotherapies as a safe and effective treatment strategy, we outline an improved method by which primary NK cells can be efficiently enriched from human peripheral blood across multiple donors (ages 20-42 years old), with a practical protocol that reliably enhances both CD56dim and CD56bright NK cells by 15-fold and 3-fold, respectively. Importantly, we show that our co-culture protocol can be used as an easily adaptable tool to assess highly efficient and selective killing of senescent cells by primary NK cells enriched via our method using longer co-culture durations and a low target to effector ratio, which may be more physiological than has been achieved in previous literature. 2022-03-04T00:00:00Z Text Journal contribution 2134/19233747.v1 https://figshare.com/articles/journal_contribution/Enhanced_co-culture_and_enrichment_of_human_natural_killer_cells_for_the_selective_clearance_of_senescent_cells/19233747 CC BY 3.0
institution	Loughborough University
collection	Figshare
topic	Animal physiology - biophysics Animal physiology - cell Animal physiology - systems aging senescence natural killer cells NKCC immune surveillance Physiology
spellingShingle	Animal physiology - biophysics Animal physiology - cell Animal physiology - systems aging senescence natural killer cells NKCC immune surveillance Physiology Kristie Kim Tesfahun Dessale Admasu Alexandra Stolzing Amit Sharma Enhanced co-culture and enrichment of human natural killer cells for the selective clearance of senescent cells
description	In the context of aging and age-associated diseases, Natural Killer (NK) cells have been revealed as a key cell type responsible for the immune clearance of senescent cells. Subsequently, NK cell-based therapies have emerged as promising alternatives to drug-based therapeutic interventions for the prevention and treatment of age-related disease and debility. Given the promise of NK cell-mediated immunotherapies as a safe and effective treatment strategy, we outline an improved method by which primary NK cells can be efficiently enriched from human peripheral blood across multiple donors (ages 20-42 years old), with a practical protocol that reliably enhances both CD56dim and CD56bright NK cells by 15-fold and 3-fold, respectively. Importantly, we show that our co-culture protocol can be used as an easily adaptable tool to assess highly efficient and selective killing of senescent cells by primary NK cells enriched via our method using longer co-culture durations and a low target to effector ratio, which may be more physiological than has been achieved in previous literature.
format	Default Article
author	Kristie Kim Tesfahun Dessale Admasu Alexandra Stolzing Amit Sharma
author_facet	Kristie Kim Tesfahun Dessale Admasu Alexandra Stolzing Amit Sharma
author_sort	Kristie Kim (12149775)
title	Enhanced co-culture and enrichment of human natural killer cells for the selective clearance of senescent cells
title_short	Enhanced co-culture and enrichment of human natural killer cells for the selective clearance of senescent cells
title_full	Enhanced co-culture and enrichment of human natural killer cells for the selective clearance of senescent cells
title_fullStr	Enhanced co-culture and enrichment of human natural killer cells for the selective clearance of senescent cells
title_full_unstemmed	Enhanced co-culture and enrichment of human natural killer cells for the selective clearance of senescent cells
title_sort	enhanced co-culture and enrichment of human natural killer cells for the selective clearance of senescent cells
publishDate	2022
url	https://hdl.handle.net/2134/19233747.v1
_version_	1797548617092825088

Enhanced co-culture and enrichment of human natural killer cells for the selective clearance of senescent cells

Similar Items