Toward acellular xenogeneic heart valve prostheses: histological and biomechanical characterization of decellularized and enzymatically deglycosylated porcine pulmonary heart valve matrices

The use of decellularized xenogeneic heart valves might offer a solution to overcome the issue of human valve shortage. The aim of this study was to revise decellularization protocols in combination with enzymatic deglycosylation, in order to reduce the immunogenicity of porcine pulmonary heart valv...

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Main Authors:	Katja Findeisen, Lucrezia Morticelli, Tobias Goecke, Louisa Kolbeck, Robert Ramm, Hans-Klaus Höffler, Gudrun Brandes, Sotiris Korossis, Axel Haverich, Andres Hilfiker
Format:	Default Article
Published:	2020
Subjects:	decellularization deglycosylation enzymatic decellularization heart valve pulmonary valve tissue engineering
Online Access:	https://hdl.handle.net/2134/13623872.v1
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spelling	rr-article-136238722020-06-18T00:00:00Z Toward acellular xenogeneic heart valve prostheses: histological and biomechanical characterization of decellularized and enzymatically deglycosylated porcine pulmonary heart valve matrices Katja Findeisen (7206650) Lucrezia Morticelli (7205276) Tobias Goecke (7211492) Louisa Kolbeck (10034795) Robert Ramm (7206647) Hans-Klaus Höffler (10034797) Gudrun Brandes (244243) Sotiris Korossis (5214488) Axel Haverich (295514) Andres Hilfiker (60280) decellularization deglycosylation enzymatic decellularization heart valve pulmonary valve tissue engineering The use of decellularized xenogeneic heart valves might offer a solution to overcome the issue of human valve shortage. The aim of this study was to revise decellularization protocols in combination with enzymatic deglycosylation, in order to reduce the immunogenicity of porcine pulmonary heart valves, in means of cells, carbohydrates, and, primarily, Galα1-3Gal (α-Gal) epitope removal. In particular, the valves were decellularized with sodium dodecylsulfate/sodium deoxycholate (SDS/SD), Triton X-100 + SDS (Tx + SDS), or Trypsin + Triton X-100 (Tryp + Tx) followed by enzymatic digestion with PNGaseF, Endoglycosidase H, or O-glycosidase combined with Neuraminidase. Results showed that decellularization alone reduced carbohydrate structures only to a limited extent, and it did not result in an α-Gal free scaffold. Nevertheless, decellularization with Tryp + Tx represented the most effective decellularization protocol in means of carbohydrates reduction. Overall, carbohydrates and α-Gal removal could strongly be improved by applying PNGaseF, in particular in combination with Tryp + Tx treatment, contrary to Endoglycosidase H and O-glycosidase treatments. Furthermore, decellularization with PNGaseF did not affect biomechanical stability, in comparison with decellularization alone, as shown by burst pressure and uniaxial tensile tests. In conclusion, valves decellularized with Tryp + Tx and PNGaseF resulted in prostheses with potentially reduced immunogenicity and maintained mechanical stability. 2020-06-18T00:00:00Z Text Journal contribution 2134/13623872.v1 https://figshare.com/articles/journal_contribution/Toward_acellular_xenogeneic_heart_valve_prostheses_histological_and_biomechanical_characterization_of_decellularized_and_enzymatically_deglycosylated_porcine_pulmonary_heart_valve_matrices/13623872 CC BY-NC-ND 4.0
institution	Loughborough University
collection	Figshare
topic	decellularization deglycosylation enzymatic decellularization heart valve pulmonary valve tissue engineering
spellingShingle	decellularization deglycosylation enzymatic decellularization heart valve pulmonary valve tissue engineering Katja Findeisen Lucrezia Morticelli Tobias Goecke Louisa Kolbeck Robert Ramm Hans-Klaus Höffler Gudrun Brandes Sotiris Korossis Axel Haverich Andres Hilfiker Toward acellular xenogeneic heart valve prostheses: histological and biomechanical characterization of decellularized and enzymatically deglycosylated porcine pulmonary heart valve matrices
description	The use of decellularized xenogeneic heart valves might offer a solution to overcome the issue of human valve shortage. The aim of this study was to revise decellularization protocols in combination with enzymatic deglycosylation, in order to reduce the immunogenicity of porcine pulmonary heart valves, in means of cells, carbohydrates, and, primarily, Galα1-3Gal (α-Gal) epitope removal. In particular, the valves were decellularized with sodium dodecylsulfate/sodium deoxycholate (SDS/SD), Triton X-100 + SDS (Tx + SDS), or Trypsin + Triton X-100 (Tryp + Tx) followed by enzymatic digestion with PNGaseF, Endoglycosidase H, or O-glycosidase combined with Neuraminidase. Results showed that decellularization alone reduced carbohydrate structures only to a limited extent, and it did not result in an α-Gal free scaffold. Nevertheless, decellularization with Tryp + Tx represented the most effective decellularization protocol in means of carbohydrates reduction. Overall, carbohydrates and α-Gal removal could strongly be improved by applying PNGaseF, in particular in combination with Tryp + Tx treatment, contrary to Endoglycosidase H and O-glycosidase treatments. Furthermore, decellularization with PNGaseF did not affect biomechanical stability, in comparison with decellularization alone, as shown by burst pressure and uniaxial tensile tests. In conclusion, valves decellularized with Tryp + Tx and PNGaseF resulted in prostheses with potentially reduced immunogenicity and maintained mechanical stability.
format	Default Article
author	Katja Findeisen Lucrezia Morticelli Tobias Goecke Louisa Kolbeck Robert Ramm Hans-Klaus Höffler Gudrun Brandes Sotiris Korossis Axel Haverich Andres Hilfiker
author_facet	Katja Findeisen Lucrezia Morticelli Tobias Goecke Louisa Kolbeck Robert Ramm Hans-Klaus Höffler Gudrun Brandes Sotiris Korossis Axel Haverich Andres Hilfiker
author_sort	Katja Findeisen (7206650)
title	Toward acellular xenogeneic heart valve prostheses: histological and biomechanical characterization of decellularized and enzymatically deglycosylated porcine pulmonary heart valve matrices
title_short	Toward acellular xenogeneic heart valve prostheses: histological and biomechanical characterization of decellularized and enzymatically deglycosylated porcine pulmonary heart valve matrices
title_full	Toward acellular xenogeneic heart valve prostheses: histological and biomechanical characterization of decellularized and enzymatically deglycosylated porcine pulmonary heart valve matrices
title_fullStr	Toward acellular xenogeneic heart valve prostheses: histological and biomechanical characterization of decellularized and enzymatically deglycosylated porcine pulmonary heart valve matrices
title_full_unstemmed	Toward acellular xenogeneic heart valve prostheses: histological and biomechanical characterization of decellularized and enzymatically deglycosylated porcine pulmonary heart valve matrices
title_sort	toward acellular xenogeneic heart valve prostheses: histological and biomechanical characterization of decellularized and enzymatically deglycosylated porcine pulmonary heart valve matrices
publishDate	2020
url	https://hdl.handle.net/2134/13623872.v1
_version_	1797186883243999232

Toward acellular xenogeneic heart valve prostheses: histological and biomechanical characterization of decellularized and enzymatically deglycosylated porcine pulmonary heart valve matrices

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