Enhanced Effects of TRAIL-endostatin-based Double-gene-radiotherapy on Suppressing Growth, Promoting Apoptosis and Inducing Cell Cycle Arrest in Vascular Endothelial Cells

This study examined the effects of TRAIL-endostatin-based gene-radiotherapy on cellu-lar growth, apoptosis and cell cycle progression in human vascular endothelial cells ECV304 in vitro. The expression of TRAIL and endostatin protein in ECV304 cells was detected by ELISA after the transfection of re...

Full description

Saved in:
Bibliographic Details
Published in:Journal of Huazhong University of Science and Technology. Medical sciences 2012-04, Vol.32 (2), p.167-172
Main Author: 李艳博 郭彩霞 王志成 龚平生 孙志伟 龚守良
Format: Article
Language:English
Subjects:
Annexin V
Apoptosis
Apoptosis - physiology
Apoptosis - radiation effects
Cell cycle
Cell Cycle Checkpoints - physiology
Cell Line
Cell Proliferation - radiation effects
Combined Modality Therapy - methods
Dose-Response Relationship, Radiation
Egr
endostatin
Endostatins - genetics
Endostatins - metabolism
Endothelial cells
Endothelial Cells - cytology
Endothelial Cells - physiology
Enzyme-linked immunosorbent assay
Flow cytometry
G1 phase
gene-radiotherapy
Genetic Therapy - methods
Humans
Ionizing radiation
Medicine
Medicine & Public Health
Plasmids
promoter
propidium iodide
Radiotherapy
Radiotherapy - methods
TNF-Related Apoptosis-Inducing Ligand - genetics
TNF-Related Apoptosis-Inducing Ligand - metabolism
TRAIL
TRAIL protein
Transfection
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:This study examined the effects of TRAIL-endostatin-based gene-radiotherapy on cellu-lar growth, apoptosis and cell cycle progression in human vascular endothelial cells ECV304 in vitro. The expression of TRAIL and endostatin protein in ECV304 cells was detected by ELISA after the transfection of recombinant plasmid pshuttle-Egr1-shTRAIL-shES and X-ray irradiation. Then MTT assay was used for determining the cellular proliferation, and flow cytometry (FCM) plus Annexin V and propidium iodide (PI) double-staining or PI single-staining were employed for the detection of apoptosis and cell cycle progression. The results showed that expression of TRAIL and endostatin protein exhibited a time- and dose-dependent change in ECV304 cells after pshut-tle-Egr1-shTRAIL-shES transfection in conjunction with irradiation. In the TRAIL-endostatin-based single- or double-gene-radiotherapy, the cell viability declined in a time- and dose-dependent manner, the percentage of cells at G2/M phase and apoptotic rate was increased, and the percentage of cells at G0/G1 phase was lowered as compared with those receiving radiotherapy alone. Moreover, TRAIL-endostatin-based double-gene-radiotherapy demonstrated better effects on growth inhibition, promotion of apoptosis and induction of cell cycle arrest in ECV304 cells than sin-gle-gene-radiotherapy.
ISSN:1672-0733
1993-1352
DOI:10.1007/s11596-012-0030-x