Visualization of long-term Mg2+ dynamics in apoptotic cells using a novel targetable fluorescent probe

Mg2+ plays important roles in many physiological processes. However, the underlying molecular mechanisms, especially in the apoptotic pathway, remain unclear due to the diffusion of Mg2+ probes, which hinders long-term imaging in specific organelles. We developed an immobilized Mg2+ probe, MGH, whic...

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Bibliographic Details
Published in:	Chemical science (Cambridge) 2017-12, Vol.8 (12), p.8255-8264
Main Authors:	Matsui, Yusuke, Funato, Yosuke, Imamura, Hiromi, Miki, Hiroaki, Mizukami, Shin, Kikuchi, Kazuya
Format:	Article
Language:	eng
Subjects:	Apoptosis Dynamics Fluorescent indicators Imaging Organelles Shrinkage
Online Access:	Get full text
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Summary:	Mg2+ plays important roles in many physiological processes. However, the underlying molecular mechanisms, especially in the apoptotic pathway, remain unclear due to the diffusion of Mg2+ probes, which hinders long-term imaging in specific organelles. We developed an immobilized Mg2+ probe, MGH, which is covalently conjugated with the HaloTag protein in various organelles. HaloTag-coupled MGH enabled long-term imaging of intracellular local Mg2+ dynamics for 24 h. To exploit this remarkable property, MGH was applied to the investigation of intracellular Mg2+ dynamics during apoptosis. Time-lapse imaging revealed an increase in the Mg2+ concentration after apoptotic cell shrinkage. Combined imaging analyses of intracellular Mg2+ and ATP concentrations strongly suggested that this Mg2+ concentration increase was caused by the dissociation of Mg2+ from ATP, along with a decrease in the intracellular ATP concentration. Thus, this protein-coupled Mg2+ probe could be a new chemical tool to elucidate intracellular Mg2+ dynamics with high spatiotemporal resolution.
ISSN:	2041-6520 2041-6539