Genetic analysis of polymerase complex(PA,PB1 and PB2) genes of H9N2 avian influenza viruses from Iran(1999 to 2009)

Objective:To determine the molecular characterization of Polymerase complex(PA,PB1 and PB2) genes of H9N2 avian influenza viruses and the genetic relalionsliip of Iranian H9N2 viruses and other Asian viruses.Methods:The Polymerase complex(PA,PBl and PB2) genes from seven isolates of H9N2 viruses iso...

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Bibliographic Details
Published in:	Asian Pacific journal of tropical biomedicine 2012-11, Vol.2 (11), p.858-862
Main Authors:	Soltanialvar, Masoud, Goodarzi, Reza, Akbarnejad, Farshad
Format:	Article
Language:	eng
Subjects:	analysis Animals Avian Avian influenza Chickens - virology complex Ducks - virology Genetic Genetic analysis Genotyping Techniques influenza Influenza A Virus, H9N2 Subtype - classification Influenza A Virus, H9N2 Subtype - genetics Influenza in Birds - virology Iran Original Pakistan Polymerase Polymerase complex RNA Replicase - genetics Viral Proteins - genetics
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Summary:	Objective:To determine the molecular characterization of Polymerase complex(PA,PB1 and PB2) genes of H9N2 avian influenza viruses and the genetic relalionsliip of Iranian H9N2 viruses and other Asian viruses.Methods:The Polymerase complex(PA,PBl and PB2) genes from seven isolates of H9N2 viruses isolated from commercial chickens in Iran during 2008-2009 were amplified(by RT-PCR method) and sequenced.Nucleotide sequences(Open Reading Frame: orf) of the PA,PBl and PB2 genes were used for phylogenetic tree construction.Results:Most PB2 and PA genes of the H9N2 viruses isolated in 2008-2009 belonged to the unknown avian sublineage which grouped with the 2004 Pakistani H7N3 viruses.The PBl genes of Iranian viruses indicated greater genetic diversity and shared a high level of similarity to PBl genes from either HS or H7 subtypes with compared to established H9N2 Eurasian sublineages.Condusions:Our findings demonstrated that the H9N2 viruses in Iran exhibit striking reassortment which has led to the generation of new genotypes.
ISSN:	2221-1691 2588-9222