Comparison of the measured values of quantitative SARS-CoV-2 spike antibody assays

•Four quantitative SARS CoV-2 spike antibody assays were compared.•The assays correlated with r-values of 0.729–0.953.•The mean antibody concentrations values of the assays varied by 1.1–2.8 times.•The quantitative SARS CoV-2 assays are not interchangeable. The concentration of antibodies against th...

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Bibliographic Details
Published in:	Journal of clinical virology 2022-10, Vol.155, p.105269-105269, Article 105269
Main Authors:	Müller, Lars, Kannenberg, Judith, Biemann, Ronald, Hönemann, Mario, Ackermann, Grit, Jassoy, Christian
Format:	Article
Language:	eng
Subjects:	Antibodies, Viral Coronavirus disease 2019 (COVID-19) COVID-19 - diagnosis Humans Immunoglobulin G Quantitative SARS-CoV-2 antibody assay SARS-CoV-2 SARS-CoV-2 spike protein Sensitivity and Specificity Severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) Spike Glycoprotein, Coronavirus - analysis Spike Glycoprotein, Coronavirus - chemistry
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Summary:	•Four quantitative SARS CoV-2 spike antibody assays were compared.•The assays correlated with r-values of 0.729–0.953.•The mean antibody concentrations values of the assays varied by 1.1–2.8 times.•The quantitative SARS CoV-2 assays are not interchangeable. The concentration of antibodies against the SARS-CoV-2 spike protein is frequently being measured for clinical and epidemiological purposes. The aim of this study was to examine whether the results of different quantitative SARS-CoV-2 spike antibody assays are comparable. The Siemens SARS-CoV-2 IgG, Abbott SARS-CoV-2 IgG II Quant, Roche ElecsysT Anti-SARS-CoV-2 S, and Euroimmun Anti-SARS-CoV-2-QuantiVac assay were compared with 110 sera from patients 6-9 months after SARS-CoV-2 infection and the WHO First International SARS-CoV-2 antibody standard 20/136. The antibody values were converted into WHO binding antibody units (BAU)/ml. The diagnostic sensitivity of the assays was determined and the antibody values were compared. The diagnostic sensitivity ranged from 57.3% (Euroimmun) to 100% (Roche). The antibody concentration values of different assays correlated with Pearson coefficients of correlation between 0.729 and 0.953. The geometric mean antibody values of the Abbott, Siemens and Euroimmun assay varied by a factor of 1.1-1.2. The geometric mean antibody values of the Roche assay were 2.4-2.8 times higher than those from the other assays. The assays yielded varying results with the WHO International antibody standard. The quantitative SARS-CoV-2 antibody assays from Abbott, Siemens, Roche and Euroimmun correlate strongly but differ in the antibody concentrations. Therefore, the same assay should be used when testing patients repeatedly. In addition, the name of the assay used and the manufacturer should be indicated along with the test results.
ISSN:	1386-6532 1873-5967