Feeding Honeybee Colonies with Honeybee-Specific Lactic Acid Bacteria (Hbs-LAB) Does Not Affect Colony-Level Hbs-LAB Composition or Paenibacillus larvae Spore Levels, Although American Foulbrood Affected Colonies Harbor a More Diverse Hbs-LAB Community

The main current methods for controlling American Foulbrood (AFB) in honeybees, caused by the bacterial pathogen Paenibacillus larvae, are enforced incineration or prophylactic antibiotic treatment, neither of which is fully satisfactory. This has led to an increased interest in the natural relation...

Full description

Saved in:
Bibliographic Details
Published in:Microbial ecology 2020-04, Vol.79 (3), p.743-755
Main Authors: Lamei, Sepideh, Stephan, Jörg G., Nilson, Bo, Sieuwerts, Sander, Riesbeck, Kristian, de Miranda, Joachim R., Forsgren, Eva
Format: Article
Language:English
Subjects:
American foulbrood
Antibiotics
Apis mellifera
Bacteria
Bees
Beneficial microbes
Bifidobacterium
Biodiversity
Biologi
Biological Sciences
Biomedical and Life Sciences
Colonies
Composition
Control methods
Direct conversion
Disease resistance
Ecology
Ekologi
Flowering
Geoecology/Natural Processes
HOST MICROBE INTERACTIONS
Incineration
Infections
Intestinal microbiota
Lactate
Lactic acid
Lactic acid bacteria
Lactobacillus
Larvae
Levels
Life Sciences
Mass spectrometry
Mass spectroscopy
Microbial Ecology
Microbiology
Microbiomes
Microorganisms
Mikrobiologi
Natural Sciences
Nature Conservation
Naturvetenskap
Paenibacillus larvae
Pathogens
Specificity
Spores
Water Quality/Water Pollution
Zoologi
Zoology
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
cited_by cdi_FETCH-LOGICAL-c604t-e462d7ec0e54a1ca3f098a5a4cb4ef04597dee15a11bdfb5a353e1a430c464ed3
cites cdi_FETCH-LOGICAL-c604t-e462d7ec0e54a1ca3f098a5a4cb4ef04597dee15a11bdfb5a353e1a430c464ed3
container_end_page 755
container_issue 3
container_start_page 743
container_title Microbial ecology
container_volume 79
creator Lamei, Sepideh
Stephan, Jörg G.
Nilson, Bo
Sieuwerts, Sander
Riesbeck, Kristian
de Miranda, Joachim R.
Forsgren, Eva
description The main current methods for controlling American Foulbrood (AFB) in honeybees, caused by the bacterial pathogen Paenibacillus larvae, are enforced incineration or prophylactic antibiotic treatment, neither of which is fully satisfactory. This has led to an increased interest in the natural relationships between the pathogenic and mutualistic microorganisms of the honeybee microbiome, in particular, the antagonistic effects of Honeybee-Specific Lactic Acid Bacteria (hbs-LAB) against P. larvae. We investigated whether supplemental administration of these bacteria affected P. larvae infection at colony level over an entire flowering season. Over the season, the supplements affected neither colony-level hbs-LAB composition nor naturally subclinical or clinical P. larvae spore levels. The composition of hbs-LAB in colonies was, however, more diverse in apiaries with a history of clinical AFB, although this was also unrelated to P. larvae spore levels. During the experiments, we also showed that qPCR could detect a wider range of hbs-LAB, with higher specificity and sensitivity than mass spectrometry. Honeybee colonies are complex super-organisms where social immune defenses, natural homeostatic mechanisms, and microbiome diversity and function play a major role in disease resistance. This means that observations made at the individual bee level cannot be simply extrapolated to infer similar effects at colony level. Although individual laboratory larval assays have clearly demonstrated the antagonistic effects of hbs-LAB on P. larvae infection, the results from the experiments presented here indicate that direct conversion of such practice to colony-level administration of live hbs-LAB is not effective. and sensitivity than mass spectrometry. Honeybee colonies are complex super-organisms where social immune defenses, natural homeostatic mechanisms, and microbiome diversity and function play a major role in disease resistance. This means that observations made at the individual bee level cannot be simply extrapolated to infer similar effects at colony level. Although individual laboratory larval assays have clearly demonstrated the antagonistic effects of hbs-LAB on P. larvae infection, the results from the experiments presented here indicate that direct conversion of such practice to colony-level administration of live hbs-LAB is not effective.
doi_str_mv 10.1007/s00248-019-01434-3
format article
fullrecord <record><control><sourceid>jstor_swepu</sourceid><recordid>TN_cdi_swepub_primary_oai_slubar_slu_se_101753</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><jstor_id>48739974</jstor_id><sourcerecordid>48739974</sourcerecordid><originalsourceid>FETCH-LOGICAL-c604t-e462d7ec0e54a1ca3f098a5a4cb4ef04597dee15a11bdfb5a353e1a430c464ed3</originalsourceid><addsrcrecordid>eNp9kl1v0zAUhiMEYmXwB5BAlrgZEhn-SpzeIHUbo0jlQxpI3Fm2c9K6cuNiJ53637nAXUq3IcGFZct-3-fYx2-WPSf4lGAs3kaMKa9yTMZpcMZz9iAbpQXNScV_PMxGGI-LnJW0OsqexLjEmIiSssfZESMFLkWJR9mvS4DatnM09S1sNQA69863FiK6tt3isJ1frcHYxho0U6ZL08TYGp2lNQSr0MlUx3w2OXuNLnyyfvYdmjQNmG7AbfMZbMChvSptrtY-2s76FvmAviporVbGOtdH5FTYKEBXax8A3fjiGzRx3cL38wWarFJBo1p06Xung_f1vhLUt1efqqATV6FPO8aF3UCIcLf6qm9tt32aPWqUi_BsPx9n3y_ffzuf5rMvHz6eT2a5KTHvcuAlrQUYDAVXxCjW4HGlCsWN5tBgXoxFDUAKRYiuG10oVjAgijNseMmhZsfZ6cCN17DutVwHu1JhK72yMrpeq7CbZARJ0hcVLBlm_zS4fp2GloPBkAo3ptay5FhJDoZKTSmVIHTFuRaiUSrh3g24xFpBbaDtgnL3qPdPWruQc7-RIgUmtSABTvaA4H_2EDu5stGAc6oF30dJaVUJxhgukvTVX9Kl70Ob2rtTiYpQwUVS0UFlgo8xQHO4DMFyF245hFumcMubcMtdV17efcbB8ifNScD2bUtH7RzCbe3_Yl8MrmXsfDhQeXrReCw4-w36ZhN2</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2287812747</pqid></control><display><type>article</type><title>Feeding Honeybee Colonies with Honeybee-Specific Lactic Acid Bacteria (Hbs-LAB) Does Not Affect Colony-Level Hbs-LAB Composition or Paenibacillus larvae Spore Levels, Although American Foulbrood Affected Colonies Harbor a More Diverse Hbs-LAB Community</title><source>JSTOR Archival Journals and Primary Sources Collection【Remote access available】</source><source>Springer Link</source><creator>Lamei, Sepideh ; Stephan, Jörg G. ; Nilson, Bo ; Sieuwerts, Sander ; Riesbeck, Kristian ; de Miranda, Joachim R. ; Forsgren, Eva</creator><creatorcontrib>Lamei, Sepideh ; Stephan, Jörg G. ; Nilson, Bo ; Sieuwerts, Sander ; Riesbeck, Kristian ; de Miranda, Joachim R. ; Forsgren, Eva ; Sveriges lantbruksuniversitet</creatorcontrib><description>The main current methods for controlling American Foulbrood (AFB) in honeybees, caused by the bacterial pathogen Paenibacillus larvae, are enforced incineration or prophylactic antibiotic treatment, neither of which is fully satisfactory. This has led to an increased interest in the natural relationships between the pathogenic and mutualistic microorganisms of the honeybee microbiome, in particular, the antagonistic effects of Honeybee-Specific Lactic Acid Bacteria (hbs-LAB) against P. larvae. We investigated whether supplemental administration of these bacteria affected P. larvae infection at colony level over an entire flowering season. Over the season, the supplements affected neither colony-level hbs-LAB composition nor naturally subclinical or clinical P. larvae spore levels. The composition of hbs-LAB in colonies was, however, more diverse in apiaries with a history of clinical AFB, although this was also unrelated to P. larvae spore levels. During the experiments, we also showed that qPCR could detect a wider range of hbs-LAB, with higher specificity and sensitivity than mass spectrometry. Honeybee colonies are complex super-organisms where social immune defenses, natural homeostatic mechanisms, and microbiome diversity and function play a major role in disease resistance. This means that observations made at the individual bee level cannot be simply extrapolated to infer similar effects at colony level. Although individual laboratory larval assays have clearly demonstrated the antagonistic effects of hbs-LAB on P. larvae infection, the results from the experiments presented here indicate that direct conversion of such practice to colony-level administration of live hbs-LAB is not effective. and sensitivity than mass spectrometry. Honeybee colonies are complex super-organisms where social immune defenses, natural homeostatic mechanisms, and microbiome diversity and function play a major role in disease resistance. This means that observations made at the individual bee level cannot be simply extrapolated to infer similar effects at colony level. Although individual laboratory larval assays have clearly demonstrated the antagonistic effects of hbs-LAB on P. larvae infection, the results from the experiments presented here indicate that direct conversion of such practice to colony-level administration of live hbs-LAB is not effective.</description><identifier>ISSN: 0095-3628</identifier><identifier>ISSN: 1432-184X</identifier><identifier>EISSN: 1432-184X</identifier><identifier>DOI: 10.1007/s00248-019-01434-3</identifier><identifier>PMID: 31506760</identifier><language>eng</language><publisher>New York: Springer Science + Business Media</publisher><subject>American foulbrood ; Antibiotics ; Apis mellifera ; Bacteria ; Bees ; Beneficial microbes ; Bifidobacterium ; Biodiversity ; Biologi ; Biological Sciences ; Biomedical and Life Sciences ; Colonies ; Composition ; Control methods ; Direct conversion ; Disease resistance ; Ecology ; Ekologi ; Flowering ; Geoecology/Natural Processes ; HOST MICROBE INTERACTIONS ; Incineration ; Infections ; Intestinal microbiota ; Lactate ; Lactic acid ; Lactic acid bacteria ; Lactobacillus ; Larvae ; Levels ; Life Sciences ; Mass spectrometry ; Mass spectroscopy ; Microbial Ecology ; Microbiology ; Microbiomes ; Microorganisms ; Mikrobiologi ; Natural Sciences ; Nature Conservation ; Naturvetenskap ; Paenibacillus larvae ; Pathogens ; Specificity ; Spores ; Water Quality/Water Pollution ; Zoologi ; Zoology</subject><ispartof>Microbial ecology, 2020-04, Vol.79 (3), p.743-755</ispartof><rights>The Author(s) 2019</rights><rights>The Author(s) 2019. This work is published under https://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c604t-e462d7ec0e54a1ca3f098a5a4cb4ef04597dee15a11bdfb5a353e1a430c464ed3</citedby><cites>FETCH-LOGICAL-c604t-e462d7ec0e54a1ca3f098a5a4cb4ef04597dee15a11bdfb5a353e1a430c464ed3</cites><orcidid>0000-0002-8103-4856 ; 0000-0001-6274-6965 ; 0000-0003-2401-6763 ; 0000-0002-8244-7265 ; 0000-0002-0335-0386 ; 0000-0001-6195-7867</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/48739974$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/48739974$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>230,315,786,790,891,27957,27958,58593,58826</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/31506760$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink><backlink>$$Uhttps://lup.lub.lu.se/record/c180fcdb-640a-4ec2-b222-e7b844b77faa$$DView record from Swedish Publication Index$$Hfree_for_read</backlink><backlink>$$Uhttps://res.slu.se/id/publ/101753$$DView record from Swedish Publication Index$$Hfree_for_read</backlink></links><search><creatorcontrib>Lamei, Sepideh</creatorcontrib><creatorcontrib>Stephan, Jörg G.</creatorcontrib><creatorcontrib>Nilson, Bo</creatorcontrib><creatorcontrib>Sieuwerts, Sander</creatorcontrib><creatorcontrib>Riesbeck, Kristian</creatorcontrib><creatorcontrib>de Miranda, Joachim R.</creatorcontrib><creatorcontrib>Forsgren, Eva</creatorcontrib><creatorcontrib>Sveriges lantbruksuniversitet</creatorcontrib><title>Feeding Honeybee Colonies with Honeybee-Specific Lactic Acid Bacteria (Hbs-LAB) Does Not Affect Colony-Level Hbs-LAB Composition or Paenibacillus larvae Spore Levels, Although American Foulbrood Affected Colonies Harbor a More Diverse Hbs-LAB Community</title><title>Microbial ecology</title><addtitle>Microb Ecol</addtitle><addtitle>Microb Ecol</addtitle><description>The main current methods for controlling American Foulbrood (AFB) in honeybees, caused by the bacterial pathogen Paenibacillus larvae, are enforced incineration or prophylactic antibiotic treatment, neither of which is fully satisfactory. This has led to an increased interest in the natural relationships between the pathogenic and mutualistic microorganisms of the honeybee microbiome, in particular, the antagonistic effects of Honeybee-Specific Lactic Acid Bacteria (hbs-LAB) against P. larvae. We investigated whether supplemental administration of these bacteria affected P. larvae infection at colony level over an entire flowering season. Over the season, the supplements affected neither colony-level hbs-LAB composition nor naturally subclinical or clinical P. larvae spore levels. The composition of hbs-LAB in colonies was, however, more diverse in apiaries with a history of clinical AFB, although this was also unrelated to P. larvae spore levels. During the experiments, we also showed that qPCR could detect a wider range of hbs-LAB, with higher specificity and sensitivity than mass spectrometry. Honeybee colonies are complex super-organisms where social immune defenses, natural homeostatic mechanisms, and microbiome diversity and function play a major role in disease resistance. This means that observations made at the individual bee level cannot be simply extrapolated to infer similar effects at colony level. Although individual laboratory larval assays have clearly demonstrated the antagonistic effects of hbs-LAB on P. larvae infection, the results from the experiments presented here indicate that direct conversion of such practice to colony-level administration of live hbs-LAB is not effective. and sensitivity than mass spectrometry. Honeybee colonies are complex super-organisms where social immune defenses, natural homeostatic mechanisms, and microbiome diversity and function play a major role in disease resistance. This means that observations made at the individual bee level cannot be simply extrapolated to infer similar effects at colony level. Although individual laboratory larval assays have clearly demonstrated the antagonistic effects of hbs-LAB on P. larvae infection, the results from the experiments presented here indicate that direct conversion of such practice to colony-level administration of live hbs-LAB is not effective.</description><subject>American foulbrood</subject><subject>Antibiotics</subject><subject>Apis mellifera</subject><subject>Bacteria</subject><subject>Bees</subject><subject>Beneficial microbes</subject><subject>Bifidobacterium</subject><subject>Biodiversity</subject><subject>Biologi</subject><subject>Biological Sciences</subject><subject>Biomedical and Life Sciences</subject><subject>Colonies</subject><subject>Composition</subject><subject>Control methods</subject><subject>Direct conversion</subject><subject>Disease resistance</subject><subject>Ecology</subject><subject>Ekologi</subject><subject>Flowering</subject><subject>Geoecology/Natural Processes</subject><subject>HOST MICROBE INTERACTIONS</subject><subject>Incineration</subject><subject>Infections</subject><subject>Intestinal microbiota</subject><subject>Lactate</subject><subject>Lactic acid</subject><subject>Lactic acid bacteria</subject><subject>Lactobacillus</subject><subject>Larvae</subject><subject>Levels</subject><subject>Life Sciences</subject><subject>Mass spectrometry</subject><subject>Mass spectroscopy</subject><subject>Microbial Ecology</subject><subject>Microbiology</subject><subject>Microbiomes</subject><subject>Microorganisms</subject><subject>Mikrobiologi</subject><subject>Natural Sciences</subject><subject>Nature Conservation</subject><subject>Naturvetenskap</subject><subject>Paenibacillus larvae</subject><subject>Pathogens</subject><subject>Specificity</subject><subject>Spores</subject><subject>Water Quality/Water Pollution</subject><subject>Zoologi</subject><subject>Zoology</subject><issn>0095-3628</issn><issn>1432-184X</issn><issn>1432-184X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><recordid>eNp9kl1v0zAUhiMEYmXwB5BAlrgZEhn-SpzeIHUbo0jlQxpI3Fm2c9K6cuNiJ53637nAXUq3IcGFZct-3-fYx2-WPSf4lGAs3kaMKa9yTMZpcMZz9iAbpQXNScV_PMxGGI-LnJW0OsqexLjEmIiSssfZESMFLkWJR9mvS4DatnM09S1sNQA69863FiK6tt3isJ1frcHYxho0U6ZL08TYGp2lNQSr0MlUx3w2OXuNLnyyfvYdmjQNmG7AbfMZbMChvSptrtY-2s76FvmAviporVbGOtdH5FTYKEBXax8A3fjiGzRx3cL38wWarFJBo1p06Xung_f1vhLUt1efqqATV6FPO8aF3UCIcLf6qm9tt32aPWqUi_BsPx9n3y_ffzuf5rMvHz6eT2a5KTHvcuAlrQUYDAVXxCjW4HGlCsWN5tBgXoxFDUAKRYiuG10oVjAgijNseMmhZsfZ6cCN17DutVwHu1JhK72yMrpeq7CbZARJ0hcVLBlm_zS4fp2GloPBkAo3ptay5FhJDoZKTSmVIHTFuRaiUSrh3g24xFpBbaDtgnL3qPdPWruQc7-RIgUmtSABTvaA4H_2EDu5stGAc6oF30dJaVUJxhgukvTVX9Kl70Ob2rtTiYpQwUVS0UFlgo8xQHO4DMFyF245hFumcMubcMtdV17efcbB8ifNScD2bUtH7RzCbe3_Yl8MrmXsfDhQeXrReCw4-w36ZhN2</recordid><startdate>20200401</startdate><enddate>20200401</enddate><creator>Lamei, Sepideh</creator><creator>Stephan, Jörg G.</creator><creator>Nilson, Bo</creator><creator>Sieuwerts, Sander</creator><creator>Riesbeck, Kristian</creator><creator>de Miranda, Joachim R.</creator><creator>Forsgren, Eva</creator><general>Springer Science + Business Media</general><general>Springer US</general><general>Springer Nature B.V</general><scope>C6C</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QL</scope><scope>7SN</scope><scope>7T7</scope><scope>7U9</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>8AO</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>BKSAR</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>F1W</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>H95</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>L.G</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>P64</scope><scope>PCBAR</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope><scope>ADTPV</scope><scope>AGCHP</scope><scope>AOWAS</scope><scope>D8T</scope><scope>D95</scope><scope>ZZAVC</scope><orcidid>https://orcid.org/0000-0002-8103-4856</orcidid><orcidid>https://orcid.org/0000-0001-6274-6965</orcidid><orcidid>https://orcid.org/0000-0003-2401-6763</orcidid><orcidid>https://orcid.org/0000-0002-8244-7265</orcidid><orcidid>https://orcid.org/0000-0002-0335-0386</orcidid><orcidid>https://orcid.org/0000-0001-6195-7867</orcidid></search><sort><creationdate>20200401</creationdate><title>Feeding Honeybee Colonies with Honeybee-Specific Lactic Acid Bacteria (Hbs-LAB) Does Not Affect Colony-Level Hbs-LAB Composition or Paenibacillus larvae Spore Levels, Although American Foulbrood Affected Colonies Harbor a More Diverse Hbs-LAB Community</title><author>Lamei, Sepideh ; Stephan, Jörg G. ; Nilson, Bo ; Sieuwerts, Sander ; Riesbeck, Kristian ; de Miranda, Joachim R. ; Forsgren, Eva</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c604t-e462d7ec0e54a1ca3f098a5a4cb4ef04597dee15a11bdfb5a353e1a430c464ed3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2020</creationdate><topic>American foulbrood</topic><topic>Antibiotics</topic><topic>Apis mellifera</topic><topic>Bacteria</topic><topic>Bees</topic><topic>Beneficial microbes</topic><topic>Bifidobacterium</topic><topic>Biodiversity</topic><topic>Biologi</topic><topic>Biological Sciences</topic><topic>Biomedical and Life Sciences</topic><topic>Colonies</topic><topic>Composition</topic><topic>Control methods</topic><topic>Direct conversion</topic><topic>Disease resistance</topic><topic>Ecology</topic><topic>Ekologi</topic><topic>Flowering</topic><topic>Geoecology/Natural Processes</topic><topic>HOST MICROBE INTERACTIONS</topic><topic>Incineration</topic><topic>Infections</topic><topic>Intestinal microbiota</topic><topic>Lactate</topic><topic>Lactic acid</topic><topic>Lactic acid bacteria</topic><topic>Lactobacillus</topic><topic>Larvae</topic><topic>Levels</topic><topic>Life Sciences</topic><topic>Mass spectrometry</topic><topic>Mass spectroscopy</topic><topic>Microbial Ecology</topic><topic>Microbiology</topic><topic>Microbiomes</topic><topic>Microorganisms</topic><topic>Mikrobiologi</topic><topic>Natural Sciences</topic><topic>Nature Conservation</topic><topic>Naturvetenskap</topic><topic>Paenibacillus larvae</topic><topic>Pathogens</topic><topic>Specificity</topic><topic>Spores</topic><topic>Water Quality/Water Pollution</topic><topic>Zoologi</topic><topic>Zoology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Lamei, Sepideh</creatorcontrib><creatorcontrib>Stephan, Jörg G.</creatorcontrib><creatorcontrib>Nilson, Bo</creatorcontrib><creatorcontrib>Sieuwerts, Sander</creatorcontrib><creatorcontrib>Riesbeck, Kristian</creatorcontrib><creatorcontrib>de Miranda, Joachim R.</creatorcontrib><creatorcontrib>Forsgren, Eva</creatorcontrib><creatorcontrib>Sveriges lantbruksuniversitet</creatorcontrib><collection>SpringerOpen</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Ecology Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Virology and AIDS Abstracts</collection><collection>ProQuest Health &amp; Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>AUTh Library subscriptions: ProQuest Central</collection><collection>ProQuest Natural Science Collection</collection><collection>Earth, Atmospheric &amp; Aquatic Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central</collection><collection>ASFA: Aquatic Sciences and Fisheries Abstracts</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Aquatic Science &amp; Fisheries Abstracts (ASFA) 1: Biological Sciences &amp; Living Resources</collection><collection>SciTech Premium Collection (Proquest) (PQ_SDU_P3)</collection><collection>ProQuest Health &amp; Medical Complete (Alumni)</collection><collection>Aquatic Science &amp; Fisheries Abstracts (ASFA) Professional</collection><collection>Biological Sciences</collection><collection>Health &amp; Medical Collection (Alumni Edition)</collection><collection>PML(ProQuest Medical Library)</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biological Science Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest Earth, Atmospheric &amp; Aquatic Science Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><collection>SwePub</collection><collection>SWEPUB Lunds universitet full text</collection><collection>SwePub Articles</collection><collection>SWEPUB Freely available online</collection><collection>SWEPUB Lunds universitet</collection><collection>SwePub Articles full text</collection><jtitle>Microbial ecology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Lamei, Sepideh</au><au>Stephan, Jörg G.</au><au>Nilson, Bo</au><au>Sieuwerts, Sander</au><au>Riesbeck, Kristian</au><au>de Miranda, Joachim R.</au><au>Forsgren, Eva</au><aucorp>Sveriges lantbruksuniversitet</aucorp><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Feeding Honeybee Colonies with Honeybee-Specific Lactic Acid Bacteria (Hbs-LAB) Does Not Affect Colony-Level Hbs-LAB Composition or Paenibacillus larvae Spore Levels, Although American Foulbrood Affected Colonies Harbor a More Diverse Hbs-LAB Community</atitle><jtitle>Microbial ecology</jtitle><stitle>Microb Ecol</stitle><addtitle>Microb Ecol</addtitle><date>2020-04-01</date><risdate>2020</risdate><volume>79</volume><issue>3</issue><spage>743</spage><epage>755</epage><pages>743-755</pages><issn>0095-3628</issn><issn>1432-184X</issn><eissn>1432-184X</eissn><notes>ObjectType-Article-1</notes><notes>SourceType-Scholarly Journals-1</notes><notes>ObjectType-Feature-2</notes><notes>content type line 23</notes><abstract>The main current methods for controlling American Foulbrood (AFB) in honeybees, caused by the bacterial pathogen Paenibacillus larvae, are enforced incineration or prophylactic antibiotic treatment, neither of which is fully satisfactory. This has led to an increased interest in the natural relationships between the pathogenic and mutualistic microorganisms of the honeybee microbiome, in particular, the antagonistic effects of Honeybee-Specific Lactic Acid Bacteria (hbs-LAB) against P. larvae. We investigated whether supplemental administration of these bacteria affected P. larvae infection at colony level over an entire flowering season. Over the season, the supplements affected neither colony-level hbs-LAB composition nor naturally subclinical or clinical P. larvae spore levels. The composition of hbs-LAB in colonies was, however, more diverse in apiaries with a history of clinical AFB, although this was also unrelated to P. larvae spore levels. During the experiments, we also showed that qPCR could detect a wider range of hbs-LAB, with higher specificity and sensitivity than mass spectrometry. Honeybee colonies are complex super-organisms where social immune defenses, natural homeostatic mechanisms, and microbiome diversity and function play a major role in disease resistance. This means that observations made at the individual bee level cannot be simply extrapolated to infer similar effects at colony level. Although individual laboratory larval assays have clearly demonstrated the antagonistic effects of hbs-LAB on P. larvae infection, the results from the experiments presented here indicate that direct conversion of such practice to colony-level administration of live hbs-LAB is not effective. and sensitivity than mass spectrometry. Honeybee colonies are complex super-organisms where social immune defenses, natural homeostatic mechanisms, and microbiome diversity and function play a major role in disease resistance. This means that observations made at the individual bee level cannot be simply extrapolated to infer similar effects at colony level. Although individual laboratory larval assays have clearly demonstrated the antagonistic effects of hbs-LAB on P. larvae infection, the results from the experiments presented here indicate that direct conversion of such practice to colony-level administration of live hbs-LAB is not effective.</abstract><cop>New York</cop><pub>Springer Science + Business Media</pub><pmid>31506760</pmid><doi>10.1007/s00248-019-01434-3</doi><tpages>13</tpages><orcidid>https://orcid.org/0000-0002-8103-4856</orcidid><orcidid>https://orcid.org/0000-0001-6274-6965</orcidid><orcidid>https://orcid.org/0000-0003-2401-6763</orcidid><orcidid>https://orcid.org/0000-0002-8244-7265</orcidid><orcidid>https://orcid.org/0000-0002-0335-0386</orcidid><orcidid>https://orcid.org/0000-0001-6195-7867</orcidid><oa>free_for_read</oa></addata></record>
fulltext fulltext
identifier ISSN: 0095-3628
ispartof Microbial ecology, 2020-04, Vol.79 (3), p.743-755
issn 0095-3628
1432-184X
1432-184X
language eng
recordid cdi_swepub_primary_oai_slubar_slu_se_101753
source JSTOR Archival Journals and Primary Sources Collection【Remote access available】; Springer Link
subjects American foulbrood
Antibiotics
Apis mellifera
Bacteria
Bees
Beneficial microbes
Bifidobacterium
Biodiversity
Biologi
Biological Sciences
Biomedical and Life Sciences
Colonies
Composition
Control methods
Direct conversion
Disease resistance
Ecology
Ekologi
Flowering
Geoecology/Natural Processes
HOST MICROBE INTERACTIONS
Incineration
Infections
Intestinal microbiota
Lactate
Lactic acid
Lactic acid bacteria
Lactobacillus
Larvae
Levels
Life Sciences
Mass spectrometry
Mass spectroscopy
Microbial Ecology
Microbiology
Microbiomes
Microorganisms
Mikrobiologi
Natural Sciences
Nature Conservation
Naturvetenskap
Paenibacillus larvae
Pathogens
Specificity
Spores
Water Quality/Water Pollution
Zoologi
Zoology
title Feeding Honeybee Colonies with Honeybee-Specific Lactic Acid Bacteria (Hbs-LAB) Does Not Affect Colony-Level Hbs-LAB Composition or Paenibacillus larvae Spore Levels, Although American Foulbrood Affected Colonies Harbor a More Diverse Hbs-LAB Community
url http://sfxeu10.hosted.exlibrisgroup.com/loughborough?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-10-01T13%3A41%3A34IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-jstor_swepu&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Feeding%20Honeybee%20Colonies%20with%20Honeybee-Specific%20Lactic%20Acid%20Bacteria%20(Hbs-LAB)%20Does%20Not%20Affect%20Colony-Level%20Hbs-LAB%20Composition%20or%20Paenibacillus%20larvae%20Spore%20Levels,%20Although%20American%20Foulbrood%20Affected%20Colonies%20Harbor%20a%20More%20Diverse%20Hbs-LAB%20Community&rft.jtitle=Microbial%20ecology&rft.au=Lamei,%20Sepideh&rft.aucorp=Sveriges%20lantbruksuniversitet&rft.date=2020-04-01&rft.volume=79&rft.issue=3&rft.spage=743&rft.epage=755&rft.pages=743-755&rft.issn=0095-3628&rft.eissn=1432-184X&rft_id=info:doi/10.1007/s00248-019-01434-3&rft_dat=%3Cjstor_swepu%3E48739974%3C/jstor_swepu%3E%3Cgrp_id%3Ecdi_FETCH-LOGICAL-c604t-e462d7ec0e54a1ca3f098a5a4cb4ef04597dee15a11bdfb5a353e1a430c464ed3%3C/grp_id%3E%3Coa%3E%3C/oa%3E%3Curl%3E%3C/url%3E&rft_id=info:oai/&rft_pqid=2287812747&rft_id=info:pmid/31506760&rft_jstor_id=48739974&rfr_iscdi=true