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A NPAS4-NuA4 complex couples synaptic activity to DNA repair
Neuronal activity is crucial for adaptive circuit remodelling but poses an inherent risk to the stability of the genome across the long lifespan of postmitotic neurons . Whether neurons have acquired specialized genome protection mechanisms that enable them to withstand decades of potentially damagi...
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| Published in: | Nature (London) 2023-02, Vol.614 (7949), p.732-741 |
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| creator | Pollina, Elizabeth A Gilliam, Daniel T Landau, Andrew T Lin, Cindy Pajarillo, Naomi Davis, Christopher P Harmin, David A Yap, Ee-Lynn Vogel, Ian R Griffith, Eric C Nagy, M Aurel Ling, Emi Duffy, Erin E Sabatini, Bernardo L Weitz, Charles J Greenberg, Michael E |
| description | Neuronal activity is crucial for adaptive circuit remodelling but poses an inherent risk to the stability of the genome across the long lifespan of postmitotic neurons
. Whether neurons have acquired specialized genome protection mechanisms that enable them to withstand decades of potentially damaging stimuli during periods of heightened activity is unknown. Here we identify an activity-dependent DNA repair mechanism in which a new form of the NuA4-TIP60 chromatin modifier assembles in activated neurons around the inducible, neuronal-specific transcription factor NPAS4. We purify this complex from the brain and demonstrate its functions in eliciting activity-dependent changes to neuronal transcriptomes and circuitry. By characterizing the landscape of activity-induced DNA double-strand breaks in the brain, we show that NPAS4-NuA4 binds to recurrently damaged regulatory elements and recruits additional DNA repair machinery to stimulate their repair. Gene regulatory elements bound by NPAS4-NuA4 are partially protected against age-dependent accumulation of somatic mutations. Impaired NPAS4-NuA4 signalling leads to a cascade of cellular defects, including dysregulated activity-dependent transcriptional responses, loss of control over neuronal inhibition and genome instability, which all culminate to reduce organismal lifespan. In addition, mutations in several components of the NuA4 complex are reported to lead to neurodevelopmental and autism spectrum disorders. Together, these findings identify a neuronal-specific complex that couples neuronal activity directly to genome preservation, the disruption of which may contribute to developmental disorders, neurodegeneration and ageing. |
| doi_str_mv | 10.1038/s41586-023-05711-7 |
| format | article |
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. Whether neurons have acquired specialized genome protection mechanisms that enable them to withstand decades of potentially damaging stimuli during periods of heightened activity is unknown. Here we identify an activity-dependent DNA repair mechanism in which a new form of the NuA4-TIP60 chromatin modifier assembles in activated neurons around the inducible, neuronal-specific transcription factor NPAS4. We purify this complex from the brain and demonstrate its functions in eliciting activity-dependent changes to neuronal transcriptomes and circuitry. By characterizing the landscape of activity-induced DNA double-strand breaks in the brain, we show that NPAS4-NuA4 binds to recurrently damaged regulatory elements and recruits additional DNA repair machinery to stimulate their repair. Gene regulatory elements bound by NPAS4-NuA4 are partially protected against age-dependent accumulation of somatic mutations. Impaired NPAS4-NuA4 signalling leads to a cascade of cellular defects, including dysregulated activity-dependent transcriptional responses, loss of control over neuronal inhibition and genome instability, which all culminate to reduce organismal lifespan. In addition, mutations in several components of the NuA4 complex are reported to lead to neurodevelopmental and autism spectrum disorders. Together, these findings identify a neuronal-specific complex that couples neuronal activity directly to genome preservation, the disruption of which may contribute to developmental disorders, neurodegeneration and ageing.</description><identifier>ISSN: 0028-0836</identifier><identifier>EISSN: 1476-4687</identifier><identifier>DOI: 10.1038/s41586-023-05711-7</identifier><identifier>PMID: 36792830</identifier><language>eng</language><publisher>England: Nature Publishing Group</publisher><subject>Aging - genetics ; Autism ; Basic Helix-Loop-Helix Transcription Factors ; Binding sites ; Brain ; Brain - metabolism ; Brain damage ; Chromatin ; Circuits ; Couples ; Deoxyribonucleic acid ; Disorders ; DNA ; DNA Breaks, Double-Stranded ; DNA damage ; DNA Repair ; Gene Expression Regulation ; Genome ; Genomes ; Genomic instability ; Life span ; Longevity - genetics ; Lysine Acetyltransferase 5 - metabolism ; Mass spectrometry ; Multiprotein Complexes - metabolism ; Mutation ; Neurodegeneration ; Neurodegenerative Diseases ; Neurodevelopmental disorders ; Neurons ; Neurons - metabolism ; Proteins ; Regulatory sequences ; Repair ; Repair & maintenance ; Scientific imaging ; Synapses - metabolism ; Transcription factors ; Transcriptomes</subject><ispartof>Nature (London), 2023-02, Vol.614 (7949), p.732-741</ispartof><rights>2023. The Author(s).</rights><rights>Copyright Nature Publishing Group Feb 23, 2023</rights><rights>The Author(s) 2023</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c430t-94e3d74aac56262d7bb243d6537f3d1e186999629666bd223e8eae5124c10eac3</citedby><cites>FETCH-LOGICAL-c430t-94e3d74aac56262d7bb243d6537f3d1e186999629666bd223e8eae5124c10eac3</cites><orcidid>0000-0003-1380-2160 ; 0000-0001-5287-0284 ; 0000-0003-0428-3215 ; 0000-0003-0095-9177 ; 0000-0002-7519-5098</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>230,315,786,790,891,27957,27958</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/36792830$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Pollina, Elizabeth A</creatorcontrib><creatorcontrib>Gilliam, Daniel T</creatorcontrib><creatorcontrib>Landau, Andrew T</creatorcontrib><creatorcontrib>Lin, Cindy</creatorcontrib><creatorcontrib>Pajarillo, Naomi</creatorcontrib><creatorcontrib>Davis, Christopher P</creatorcontrib><creatorcontrib>Harmin, David A</creatorcontrib><creatorcontrib>Yap, Ee-Lynn</creatorcontrib><creatorcontrib>Vogel, Ian R</creatorcontrib><creatorcontrib>Griffith, Eric C</creatorcontrib><creatorcontrib>Nagy, M Aurel</creatorcontrib><creatorcontrib>Ling, Emi</creatorcontrib><creatorcontrib>Duffy, Erin E</creatorcontrib><creatorcontrib>Sabatini, Bernardo L</creatorcontrib><creatorcontrib>Weitz, Charles J</creatorcontrib><creatorcontrib>Greenberg, Michael E</creatorcontrib><title>A NPAS4-NuA4 complex couples synaptic activity to DNA repair</title><title>Nature (London)</title><addtitle>Nature</addtitle><description>Neuronal activity is crucial for adaptive circuit remodelling but poses an inherent risk to the stability of the genome across the long lifespan of postmitotic neurons
. Whether neurons have acquired specialized genome protection mechanisms that enable them to withstand decades of potentially damaging stimuli during periods of heightened activity is unknown. Here we identify an activity-dependent DNA repair mechanism in which a new form of the NuA4-TIP60 chromatin modifier assembles in activated neurons around the inducible, neuronal-specific transcription factor NPAS4. We purify this complex from the brain and demonstrate its functions in eliciting activity-dependent changes to neuronal transcriptomes and circuitry. By characterizing the landscape of activity-induced DNA double-strand breaks in the brain, we show that NPAS4-NuA4 binds to recurrently damaged regulatory elements and recruits additional DNA repair machinery to stimulate their repair. Gene regulatory elements bound by NPAS4-NuA4 are partially protected against age-dependent accumulation of somatic mutations. Impaired NPAS4-NuA4 signalling leads to a cascade of cellular defects, including dysregulated activity-dependent transcriptional responses, loss of control over neuronal inhibition and genome instability, which all culminate to reduce organismal lifespan. In addition, mutations in several components of the NuA4 complex are reported to lead to neurodevelopmental and autism spectrum disorders. 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. Whether neurons have acquired specialized genome protection mechanisms that enable them to withstand decades of potentially damaging stimuli during periods of heightened activity is unknown. Here we identify an activity-dependent DNA repair mechanism in which a new form of the NuA4-TIP60 chromatin modifier assembles in activated neurons around the inducible, neuronal-specific transcription factor NPAS4. We purify this complex from the brain and demonstrate its functions in eliciting activity-dependent changes to neuronal transcriptomes and circuitry. By characterizing the landscape of activity-induced DNA double-strand breaks in the brain, we show that NPAS4-NuA4 binds to recurrently damaged regulatory elements and recruits additional DNA repair machinery to stimulate their repair. Gene regulatory elements bound by NPAS4-NuA4 are partially protected against age-dependent accumulation of somatic mutations. Impaired NPAS4-NuA4 signalling leads to a cascade of cellular defects, including dysregulated activity-dependent transcriptional responses, loss of control over neuronal inhibition and genome instability, which all culminate to reduce organismal lifespan. In addition, mutations in several components of the NuA4 complex are reported to lead to neurodevelopmental and autism spectrum disorders. Together, these findings identify a neuronal-specific complex that couples neuronal activity directly to genome preservation, the disruption of which may contribute to developmental disorders, neurodegeneration and ageing.</abstract><cop>England</cop><pub>Nature Publishing Group</pub><pmid>36792830</pmid><doi>10.1038/s41586-023-05711-7</doi><tpages>10</tpages><orcidid>https://orcid.org/0000-0003-1380-2160</orcidid><orcidid>https://orcid.org/0000-0001-5287-0284</orcidid><orcidid>https://orcid.org/0000-0003-0428-3215</orcidid><orcidid>https://orcid.org/0000-0003-0095-9177</orcidid><orcidid>https://orcid.org/0000-0002-7519-5098</orcidid><oa>free_for_read</oa></addata></record> |
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| ispartof | Nature (London), 2023-02, Vol.614 (7949), p.732-741 |
| issn | 0028-0836 1476-4687 |
| language | eng |
| recordid | cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_9946837 |
| source | Springer Nature - Connect here FIRST to enable access |
| subjects | Aging - genetics Autism Basic Helix-Loop-Helix Transcription Factors Binding sites Brain Brain - metabolism Brain damage Chromatin Circuits Couples Deoxyribonucleic acid Disorders DNA DNA Breaks, Double-Stranded DNA damage DNA Repair Gene Expression Regulation Genome Genomes Genomic instability Life span Longevity - genetics Lysine Acetyltransferase 5 - metabolism Mass spectrometry Multiprotein Complexes - metabolism Mutation Neurodegeneration Neurodegenerative Diseases Neurodevelopmental disorders Neurons Neurons - metabolism Proteins Regulatory sequences Repair Repair & maintenance Scientific imaging Synapses - metabolism Transcription factors Transcriptomes |
| title | A NPAS4-NuA4 complex couples synaptic activity to DNA repair |
| url | http://sfxeu10.hosted.exlibrisgroup.com/loughborough?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-09-22T02%3A29%3A18IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_pubme&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=A%20NPAS4-NuA4%20complex%20couples%20synaptic%20activity%20to%20DNA%20repair&rft.jtitle=Nature%20(London)&rft.au=Pollina,%20Elizabeth%20A&rft.date=2023-02-23&rft.volume=614&rft.issue=7949&rft.spage=732&rft.epage=741&rft.pages=732-741&rft.issn=0028-0836&rft.eissn=1476-4687&rft_id=info:doi/10.1038/s41586-023-05711-7&rft_dat=%3Cproquest_pubme%3E2777405315%3C/proquest_pubme%3E%3Cgrp_id%3Ecdi_FETCH-LOGICAL-c430t-94e3d74aac56262d7bb243d6537f3d1e186999629666bd223e8eae5124c10eac3%3C/grp_id%3E%3Coa%3E%3C/oa%3E%3Curl%3E%3C/url%3E&rft_id=info:oai/&rft_pqid=2779947973&rft_id=info:pmid/36792830&rfr_iscdi=true |