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The Role of Combining Probiotics in Preventing and Controlling Inflammation: A Focus on the Anti-Inflammatory and Immunomodulatory Effects of Probiotics in an In Vitro Model of IBD
Objective. IBD is an inflammatory disease with abnormalities such as dysbiosis and abnormal immune system activity. Probiotics, as live beneficial microorganisms, play a role in maintaining health through various mechanisms, including the modulation of the immune system and the control of inflammati...
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Published in: | Canadian Journal of Gastroenterology and Hepatology 2022, Vol.2022, p.1-9 |
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creator | Aghamohammad, Shadi Sepehr, Amin Miri, Seyedeh Tina Najafi, Saeideh Pourshafie, Mohammad R. Rohani, Mahdi |
description | Objective. IBD is an inflammatory disease with abnormalities such as dysbiosis and abnormal immune system activity. Probiotics, as live beneficial microorganisms, play a role in maintaining health through various mechanisms, including the modulation of the immune system and the control of inflammation. Here, we aimed to investigate the efficacy of a probiotic mixture of Lactobacillus spp. and Bifidobacterium spp. in modulating JAK/STAT and NF-kB inflammatory signaling pathways. Method. A quantitative real-time polymerase chain reaction (qPCR) assay was conducted to analyze the expression of JAK/STAT and inflammatory genes after treatment with the probiotic mixture before, after, and simultaneously with the sonicated pathogen in the HT-29 cell line. The production of IL-6 and IL-1β after probiotic treatment was investigated via cytokine assay. Results. Treatment with probiotics resulted in downregulation of TIRAP, IRAK4, NEMO, and RIP genes in the NF-kB pathway and JAK/STAT genes compared with sonicat-treated cells as inflammation inducers. The production of IL-6 and IL-1 decreased after probiotic treatment. Conclusions. The probiotic mixture of Lactobacillus spp. and Bifidobacterium spp. showed anti-inflammatory effects by modulating JAK/STAT and NF-kB signaling pathways. The use of probiotics could be considered as an appropriate complementary treatment for patients with inflammatory bowel disease. |
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IBD is an inflammatory disease with abnormalities such as dysbiosis and abnormal immune system activity. Probiotics, as live beneficial microorganisms, play a role in maintaining health through various mechanisms, including the modulation of the immune system and the control of inflammation. Here, we aimed to investigate the efficacy of a probiotic mixture of Lactobacillus spp. and Bifidobacterium spp. in modulating JAK/STAT and NF-kB inflammatory signaling pathways. Method. A quantitative real-time polymerase chain reaction (qPCR) assay was conducted to analyze the expression of JAK/STAT and inflammatory genes after treatment with the probiotic mixture before, after, and simultaneously with the sonicated pathogen in the HT-29 cell line. The production of IL-6 and IL-1β after probiotic treatment was investigated via cytokine assay. Results. Treatment with probiotics resulted in downregulation of TIRAP, IRAK4, NEMO, and RIP genes in the NF-kB pathway and JAK/STAT genes compared with sonicat-treated cells as inflammation inducers. The production of IL-6 and IL-1 decreased after probiotic treatment. Conclusions. The probiotic mixture of Lactobacillus spp. and Bifidobacterium spp. showed anti-inflammatory effects by modulating JAK/STAT and NF-kB signaling pathways. The use of probiotics could be considered as an appropriate complementary treatment for patients with inflammatory bowel disease.</description><identifier>ISSN: 2291-2789</identifier><identifier>EISSN: 2291-2797</identifier><identifier>DOI: 10.1155/2022/2045572</identifier><language>eng ; fre</language><publisher>Oakville: Hindawi</publisher><subject>Bacteria ; Cell culture ; Cytokines ; Disease prevention ; Gene expression ; Immune system ; Inflammation ; Inflammatory bowel disease ; Inflammatory diseases ; Kinases ; Pathogens ; Probiotics ; Remission (Medicine) ; Salmonella</subject><ispartof>Canadian Journal of Gastroenterology and Hepatology, 2022, Vol.2022, p.1-9</ispartof><rights>Copyright © 2022 Shadi Aghamohammad et al.</rights><rights>Copyright © 2022 Shadi Aghamohammad et al. This is an open access article distributed under the Creative Commons Attribution License (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License. https://creativecommons.org/licenses/by/4.0</rights><rights>Copyright © 2022 Shadi Aghamohammad et al. 2022</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c491t-82980f8896aa02cdafcf09a014633ef3400a0ca34b84314edb3dd36fe1ecaea93</citedby><cites>FETCH-LOGICAL-c491t-82980f8896aa02cdafcf09a014633ef3400a0ca34b84314edb3dd36fe1ecaea93</cites><orcidid>0000-0001-6666-2005 ; 0000-0001-8956-5072 ; 0000-0002-5520-5711 ; 0000-0002-6226-7064 ; 0000-0003-1832-3695 ; 0000-0002-0941-4432</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.proquest.com/docview/2737948178/fulltextPDF?pq-origsite=primo$$EPDF$$P50$$Gproquest$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.proquest.com/docview/2737948178?pq-origsite=primo$$EHTML$$P50$$Gproquest$$Hfree_for_read</linktohtml><link.rule.ids>230,315,733,786,790,891,4043,25783,27956,27957,27958,37047,44625,53827,53829,75483</link.rule.ids></links><search><contributor>Caspa Gokulan, Ravindran</contributor><contributor>Ravindran Caspa Gokulan</contributor><creatorcontrib>Aghamohammad, Shadi</creatorcontrib><creatorcontrib>Sepehr, Amin</creatorcontrib><creatorcontrib>Miri, Seyedeh Tina</creatorcontrib><creatorcontrib>Najafi, Saeideh</creatorcontrib><creatorcontrib>Pourshafie, Mohammad R.</creatorcontrib><creatorcontrib>Rohani, Mahdi</creatorcontrib><title>The Role of Combining Probiotics in Preventing and Controlling Inflammation: A Focus on the Anti-Inflammatory and Immunomodulatory Effects of Probiotics in an In Vitro Model of IBD</title><title>Canadian Journal of Gastroenterology and Hepatology</title><description>Objective. IBD is an inflammatory disease with abnormalities such as dysbiosis and abnormal immune system activity. Probiotics, as live beneficial microorganisms, play a role in maintaining health through various mechanisms, including the modulation of the immune system and the control of inflammation. Here, we aimed to investigate the efficacy of a probiotic mixture of Lactobacillus spp. and Bifidobacterium spp. in modulating JAK/STAT and NF-kB inflammatory signaling pathways. Method. A quantitative real-time polymerase chain reaction (qPCR) assay was conducted to analyze the expression of JAK/STAT and inflammatory genes after treatment with the probiotic mixture before, after, and simultaneously with the sonicated pathogen in the HT-29 cell line. The production of IL-6 and IL-1β after probiotic treatment was investigated via cytokine assay. Results. Treatment with probiotics resulted in downregulation of TIRAP, IRAK4, NEMO, and RIP genes in the NF-kB pathway and JAK/STAT genes compared with sonicat-treated cells as inflammation inducers. The production of IL-6 and IL-1 decreased after probiotic treatment. Conclusions. The probiotic mixture of Lactobacillus spp. and Bifidobacterium spp. showed anti-inflammatory effects by modulating JAK/STAT and NF-kB signaling pathways. 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Sepehr, Amin ; Miri, Seyedeh Tina ; Najafi, Saeideh ; Pourshafie, Mohammad R. ; Rohani, Mahdi</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c491t-82980f8896aa02cdafcf09a014633ef3400a0ca34b84314edb3dd36fe1ecaea93</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng ; fre</language><creationdate>2022</creationdate><topic>Bacteria</topic><topic>Cell culture</topic><topic>Cytokines</topic><topic>Disease prevention</topic><topic>Gene expression</topic><topic>Immune system</topic><topic>Inflammation</topic><topic>Inflammatory bowel disease</topic><topic>Inflammatory diseases</topic><topic>Kinases</topic><topic>Pathogens</topic><topic>Probiotics</topic><topic>Remission (Medicine)</topic><topic>Salmonella</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Aghamohammad, Shadi</creatorcontrib><creatorcontrib>Sepehr, Amin</creatorcontrib><creatorcontrib>Miri, Seyedeh Tina</creatorcontrib><creatorcontrib>Najafi, Saeideh</creatorcontrib><creatorcontrib>Pourshafie, Mohammad R.</creatorcontrib><creatorcontrib>Rohani, Mahdi</creatorcontrib><collection>Hindawi Publishing Complete</collection><collection>Hindawi Publishing Subscription Journals</collection><collection>Hindawi Publishing Open Access Journals</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Health & Medical Collection (Proquest)</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Canadian Business & Current Affairs Database</collection><collection>Canadian Business & Current Affairs Database (Alumni Edition)</collection><collection>ProQuest Central (Alumni)</collection><collection>ProQuest Central</collection><collection>ProQuest Central Essentials</collection><collection>AUTh Library subscriptions: ProQuest Central</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>PML(ProQuest Medical Library)</collection><collection>ProQuest - Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>ProQuest Central Basic</collection><collection>PubMed Central (Full Participant titles)</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>Canadian Journal of Gastroenterology and Hepatology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Aghamohammad, Shadi</au><au>Sepehr, Amin</au><au>Miri, Seyedeh Tina</au><au>Najafi, Saeideh</au><au>Pourshafie, Mohammad R.</au><au>Rohani, Mahdi</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The Role of Combining Probiotics in Preventing and Controlling Inflammation: A Focus on the Anti-Inflammatory and Immunomodulatory Effects of Probiotics in an In Vitro Model of IBD</atitle><jtitle>Canadian Journal of Gastroenterology and Hepatology</jtitle><date>2022</date><risdate>2022</risdate><volume>2022</volume><spage>1</spage><epage>9</epage><pages>1-9</pages><issn>2291-2789</issn><eissn>2291-2797</eissn><notes>Academic Editor: Ravindran Caspa Gokulan</notes><abstract>Objective. IBD is an inflammatory disease with abnormalities such as dysbiosis and abnormal immune system activity. Probiotics, as live beneficial microorganisms, play a role in maintaining health through various mechanisms, including the modulation of the immune system and the control of inflammation. Here, we aimed to investigate the efficacy of a probiotic mixture of Lactobacillus spp. and Bifidobacterium spp. in modulating JAK/STAT and NF-kB inflammatory signaling pathways. Method. A quantitative real-time polymerase chain reaction (qPCR) assay was conducted to analyze the expression of JAK/STAT and inflammatory genes after treatment with the probiotic mixture before, after, and simultaneously with the sonicated pathogen in the HT-29 cell line. The production of IL-6 and IL-1β after probiotic treatment was investigated via cytokine assay. Results. Treatment with probiotics resulted in downregulation of TIRAP, IRAK4, NEMO, and RIP genes in the NF-kB pathway and JAK/STAT genes compared with sonicat-treated cells as inflammation inducers. The production of IL-6 and IL-1 decreased after probiotic treatment. Conclusions. The probiotic mixture of Lactobacillus spp. and Bifidobacterium spp. showed anti-inflammatory effects by modulating JAK/STAT and NF-kB signaling pathways. The use of probiotics could be considered as an appropriate complementary treatment for patients with inflammatory bowel disease.</abstract><cop>Oakville</cop><pub>Hindawi</pub><doi>10.1155/2022/2045572</doi><tpages>9</tpages><orcidid>https://orcid.org/0000-0001-6666-2005</orcidid><orcidid>https://orcid.org/0000-0001-8956-5072</orcidid><orcidid>https://orcid.org/0000-0002-5520-5711</orcidid><orcidid>https://orcid.org/0000-0002-6226-7064</orcidid><orcidid>https://orcid.org/0000-0003-1832-3695</orcidid><orcidid>https://orcid.org/0000-0002-0941-4432</orcidid><oa>free_for_read</oa></addata></record> |
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subjects | Bacteria Cell culture Cytokines Disease prevention Gene expression Immune system Inflammation Inflammatory bowel disease Inflammatory diseases Kinases Pathogens Probiotics Remission (Medicine) Salmonella |
title | The Role of Combining Probiotics in Preventing and Controlling Inflammation: A Focus on the Anti-Inflammatory and Immunomodulatory Effects of Probiotics in an In Vitro Model of IBD |
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