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Organization and transcriptional analysis of a six-gene cluster around the rplK-rplA operon of Corynebacterium glutamicum encoding the ribosomal proteins L11 and L1
A cluster of six genes, tRNA(Trp)-secE-nusG-rplK-rplA-pkwR, was cloned and sequenced from a Corynebacterium glutamicum cosmid library and shown to be contiguous in the C. glutamicum genome. These genes encode a tryptophanyl tRNA, the protein translocase component SecE, the antiterminator protein Nus...
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| Published in: | Applied and environmental microbiology 2001-05, Vol.67 (5), p.2183-2190 |
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| description | A cluster of six genes, tRNA(Trp)-secE-nusG-rplK-rplA-pkwR, was cloned and sequenced from a Corynebacterium glutamicum cosmid library and shown to be contiguous in the C. glutamicum genome. These genes encode a tryptophanyl tRNA, the protein translocase component SecE, the antiterminator protein NusG, and the ribosomal proteins L11 and L1 in addition to PkwR, a putative regulatory protein of the LacI-GalR family. S1 nuclease mapping analysis revealed that nusG and rplK are expressed as separate transcriptional units and rplK and rplA are cotranscribed as a single mRNA. A 19-nucleotide inverted repeat that appears to correspond to a transcriptional terminator was located in the 3' region downstream from nusG. Northern analysis with different probes confirmed the S1 mapping results and showed that the secE-rplA four-gene region gives rise to four transcripts. secE was transcribed as a 0.5-kb monocistronic mRNA, nusG formed two transcripts of 1.4 and 1.0 kb from different initiation sites, and the two ribosomal protein genes rplK and rplA were cotranscribed as a single mRNA of 1.6 kb. A consensus L1 protein binding sequence was identified in the leader region of the rplK-rplA transcript, suggesting that expression of the rplK-rplA cluster was regulated by autogenous regulation exerted by the L1 protein at the translation level. The promoters of the nusG and rplK-rplA genes were subcloned in a novel corynebacterial promoter-probe vector and shown to confer strong expression of the reporter gene. |
| doi_str_mv | 10.1128/AEM.67.5.2183-2190.2001 |
| format | article |
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These genes encode a tryptophanyl tRNA, the protein translocase component SecE, the antiterminator protein NusG, and the ribosomal proteins L11 and L1 in addition to PkwR, a putative regulatory protein of the LacI-GalR family. S1 nuclease mapping analysis revealed that nusG and rplK are expressed as separate transcriptional units and rplK and rplA are cotranscribed as a single mRNA. A 19-nucleotide inverted repeat that appears to correspond to a transcriptional terminator was located in the 3' region downstream from nusG. Northern analysis with different probes confirmed the S1 mapping results and showed that the secE-rplA four-gene region gives rise to four transcripts. secE was transcribed as a 0.5-kb monocistronic mRNA, nusG formed two transcripts of 1.4 and 1.0 kb from different initiation sites, and the two ribosomal protein genes rplK and rplA were cotranscribed as a single mRNA of 1.6 kb. A consensus L1 protein binding sequence was identified in the leader region of the rplK-rplA transcript, suggesting that expression of the rplK-rplA cluster was regulated by autogenous regulation exerted by the L1 protein at the translation level. The promoters of the nusG and rplK-rplA genes were subcloned in a novel corynebacterial promoter-probe vector and shown to confer strong expression of the reporter gene.</description><identifier>ISSN: 0099-2240</identifier><identifier>EISSN: 1098-5336</identifier><identifier>DOI: 10.1128/AEM.67.5.2183-2190.2001</identifier><identifier>PMID: 11319098</identifier><identifier>CODEN: AEMIDF</identifier><language>eng</language><publisher>Washington, DC: American Society for Microbiology</publisher><subject>Bacteria ; Bacteriology ; Base Sequence ; Biological and medical sciences ; Blotting, Northern ; Cloning, Molecular ; Corynebacterium - genetics ; Corynebacterium - growth & development ; Corynebacterium - metabolism ; Corynebacterium glutamicum ; Fundamental and applied biological sciences. Psychology ; Gene Expression Regulation, Bacterial ; Genes ; Genes, Bacterial ; Genetics ; Genetics and Molecular Biology ; Genomics ; Microbiology ; Molecular Sequence Data ; Multigene Family ; nusG gene ; Operon - genetics ; pkwR gene ; Plasmids - genetics ; Promoter Regions, Genetic - genetics ; Proteins ; Ribonucleic acid ; ribosomal protein L1 ; ribosomal protein L11 ; Ribosomal Proteins - genetics ; Ribosomal Proteins - metabolism ; RNA ; rplA gene ; rplK gene ; secE gene ; Sequence Analysis, DNA ; Transcription, Genetic ; tRNA Trp</subject><ispartof>Applied and environmental microbiology, 2001-05, Vol.67 (5), p.2183-2190</ispartof><rights>2001 INIST-CNRS</rights><rights>Copyright American Society for Microbiology May 2001</rights><rights>Copyright © 2001, American Society for Microbiology 2001</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c498t-bb95a8d99952587ff41fed98bdc58efc7c680c4cf99cc37c9fa68d8a6c037e6b3</citedby><cites>FETCH-LOGICAL-c498t-bb95a8d99952587ff41fed98bdc58efc7c680c4cf99cc37c9fa68d8a6c037e6b3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC92853/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC92853/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,315,733,786,790,891,3207,27957,27958,53827,53829</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=1070051$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/11319098$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>BARREIRO, Carlos</creatorcontrib><creatorcontrib>GONZALEZ-LAVADO, Eva</creatorcontrib><creatorcontrib>MARTIN, Juan F</creatorcontrib><title>Organization and transcriptional analysis of a six-gene cluster around the rplK-rplA operon of Corynebacterium glutamicum encoding the ribosomal proteins L11 and L1</title><title>Applied and environmental microbiology</title><addtitle>Appl Environ Microbiol</addtitle><description>A cluster of six genes, tRNA(Trp)-secE-nusG-rplK-rplA-pkwR, was cloned and sequenced from a Corynebacterium glutamicum cosmid library and shown to be contiguous in the C. glutamicum genome. These genes encode a tryptophanyl tRNA, the protein translocase component SecE, the antiterminator protein NusG, and the ribosomal proteins L11 and L1 in addition to PkwR, a putative regulatory protein of the LacI-GalR family. S1 nuclease mapping analysis revealed that nusG and rplK are expressed as separate transcriptional units and rplK and rplA are cotranscribed as a single mRNA. A 19-nucleotide inverted repeat that appears to correspond to a transcriptional terminator was located in the 3' region downstream from nusG. Northern analysis with different probes confirmed the S1 mapping results and showed that the secE-rplA four-gene region gives rise to four transcripts. secE was transcribed as a 0.5-kb monocistronic mRNA, nusG formed two transcripts of 1.4 and 1.0 kb from different initiation sites, and the two ribosomal protein genes rplK and rplA were cotranscribed as a single mRNA of 1.6 kb. A consensus L1 protein binding sequence was identified in the leader region of the rplK-rplA transcript, suggesting that expression of the rplK-rplA cluster was regulated by autogenous regulation exerted by the L1 protein at the translation level. The promoters of the nusG and rplK-rplA genes were subcloned in a novel corynebacterial promoter-probe vector and shown to confer strong expression of the reporter gene.</description><subject>Bacteria</subject><subject>Bacteriology</subject><subject>Base Sequence</subject><subject>Biological and medical sciences</subject><subject>Blotting, Northern</subject><subject>Cloning, Molecular</subject><subject>Corynebacterium - genetics</subject><subject>Corynebacterium - growth & development</subject><subject>Corynebacterium - metabolism</subject><subject>Corynebacterium glutamicum</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gene Expression Regulation, Bacterial</subject><subject>Genes</subject><subject>Genes, Bacterial</subject><subject>Genetics</subject><subject>Genetics and Molecular Biology</subject><subject>Genomics</subject><subject>Microbiology</subject><subject>Molecular Sequence Data</subject><subject>Multigene Family</subject><subject>nusG gene</subject><subject>Operon - genetics</subject><subject>pkwR gene</subject><subject>Plasmids - genetics</subject><subject>Promoter Regions, Genetic - genetics</subject><subject>Proteins</subject><subject>Ribonucleic acid</subject><subject>ribosomal protein L1</subject><subject>ribosomal protein L11</subject><subject>Ribosomal Proteins - genetics</subject><subject>Ribosomal Proteins - metabolism</subject><subject>RNA</subject><subject>rplA gene</subject><subject>rplK gene</subject><subject>secE gene</subject><subject>Sequence Analysis, DNA</subject><subject>Transcription, Genetic</subject><subject>tRNA Trp</subject><issn>0099-2240</issn><issn>1098-5336</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2001</creationdate><recordtype>article</recordtype><recordid>eNqFkl2P1CAUhonRuLOjf0GJMXvXeqAfQOLNZLJ-xDF7o9eEUtpl00KF1jj-Hn-o1Jno6o03QA7Pe87h8CL0nEBOCOWvdtcf85rlVU4JLzJKBOQUgDxAGwKCZ1VR1A_RBkCIjNISLtBljHcAUELNH6MLQookEXyDftyEXjn7Xc3WO6xci-egXNTBTmtEDSmmhmO0EfsOKxztt6w3zmA9LHE2Aavgl1V1a3CYhg9ZWnbYTyakdEmx9-HoTKN0Yu0y4n5YZjVanY7Gad9a15-0tvHRj6neFPxsrIv4QMivhg7kCXrUqSGap-d9iz6_uf60f5cdbt6-3-8OmS4Fn7OmEZXirRCiohVnXVeSzrSCN62uuOk00zUHXepOCK0LpkWnat5yVWsomKmbYoten_JOSzOaVhuXhjHIKdhRhaP0ysq_b5y9lb3_KgXlaeRbdHWWB_9lMXGWo43aDINyxi9RMuAANfD_goQJoIySBL74B7zzS0gfEiWFStQFLSBB7ATp4GMMpvvdMAG5ukUmt8iayUqubpGrW-TqlqR8dv-9f3RneyTg5RlQUauhS9bQNt7LzwAqUvwEO3zNDg</recordid><startdate>20010501</startdate><enddate>20010501</enddate><creator>BARREIRO, Carlos</creator><creator>GONZALEZ-LAVADO, Eva</creator><creator>MARTIN, Juan F</creator><general>American Society for Microbiology</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7QO</scope><scope>7SN</scope><scope>7SS</scope><scope>7ST</scope><scope>7T7</scope><scope>7TM</scope><scope>7U9</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope><scope>SOI</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20010501</creationdate><title>Organization and transcriptional analysis of a six-gene cluster around the rplK-rplA operon of Corynebacterium glutamicum encoding the ribosomal proteins L11 and L1</title><author>BARREIRO, Carlos ; GONZALEZ-LAVADO, Eva ; MARTIN, Juan F</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c498t-bb95a8d99952587ff41fed98bdc58efc7c680c4cf99cc37c9fa68d8a6c037e6b3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2001</creationdate><topic>Bacteria</topic><topic>Bacteriology</topic><topic>Base Sequence</topic><topic>Biological and medical sciences</topic><topic>Blotting, Northern</topic><topic>Cloning, Molecular</topic><topic>Corynebacterium - genetics</topic><topic>Corynebacterium - growth & development</topic><topic>Corynebacterium - metabolism</topic><topic>Corynebacterium glutamicum</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gene Expression Regulation, Bacterial</topic><topic>Genes</topic><topic>Genes, Bacterial</topic><topic>Genetics</topic><topic>Genetics and Molecular Biology</topic><topic>Genomics</topic><topic>Microbiology</topic><topic>Molecular Sequence Data</topic><topic>Multigene Family</topic><topic>nusG gene</topic><topic>Operon - genetics</topic><topic>pkwR gene</topic><topic>Plasmids - genetics</topic><topic>Promoter Regions, Genetic - genetics</topic><topic>Proteins</topic><topic>Ribonucleic acid</topic><topic>ribosomal protein L1</topic><topic>ribosomal protein L11</topic><topic>Ribosomal Proteins - genetics</topic><topic>Ribosomal Proteins - metabolism</topic><topic>RNA</topic><topic>rplA gene</topic><topic>rplK gene</topic><topic>secE gene</topic><topic>Sequence Analysis, DNA</topic><topic>Transcription, Genetic</topic><topic>tRNA Trp</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>BARREIRO, Carlos</creatorcontrib><creatorcontrib>GONZALEZ-LAVADO, Eva</creatorcontrib><creatorcontrib>MARTIN, Juan F</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Biotechnology Research Abstracts</collection><collection>Ecology Abstracts</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Environment Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>Environment Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Applied and environmental microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>BARREIRO, Carlos</au><au>GONZALEZ-LAVADO, Eva</au><au>MARTIN, Juan F</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Organization and transcriptional analysis of a six-gene cluster around the rplK-rplA operon of Corynebacterium glutamicum encoding the ribosomal proteins L11 and L1</atitle><jtitle>Applied and environmental microbiology</jtitle><addtitle>Appl Environ Microbiol</addtitle><date>2001-05-01</date><risdate>2001</risdate><volume>67</volume><issue>5</issue><spage>2183</spage><epage>2190</epage><pages>2183-2190</pages><issn>0099-2240</issn><eissn>1098-5336</eissn><coden>AEMIDF</coden><notes>ObjectType-Article-2</notes><notes>SourceType-Scholarly Journals-1</notes><notes>ObjectType-Feature-1</notes><notes>content type line 23</notes><notes>ObjectType-Article-1</notes><notes>ObjectType-Feature-2</notes><notes>Corresponding author. Mailing address: Instituto de Biotecnología (INBIOTEC), Parque Científico de León, Avda. del Real, no. 1, 24006 León, Spain. Phone: (34 987) 210308. Fax: (34 987) 210388. E-mail: degjmm@unileon.es.</notes><abstract>A cluster of six genes, tRNA(Trp)-secE-nusG-rplK-rplA-pkwR, was cloned and sequenced from a Corynebacterium glutamicum cosmid library and shown to be contiguous in the C. glutamicum genome. These genes encode a tryptophanyl tRNA, the protein translocase component SecE, the antiterminator protein NusG, and the ribosomal proteins L11 and L1 in addition to PkwR, a putative regulatory protein of the LacI-GalR family. S1 nuclease mapping analysis revealed that nusG and rplK are expressed as separate transcriptional units and rplK and rplA are cotranscribed as a single mRNA. A 19-nucleotide inverted repeat that appears to correspond to a transcriptional terminator was located in the 3' region downstream from nusG. Northern analysis with different probes confirmed the S1 mapping results and showed that the secE-rplA four-gene region gives rise to four transcripts. secE was transcribed as a 0.5-kb monocistronic mRNA, nusG formed two transcripts of 1.4 and 1.0 kb from different initiation sites, and the two ribosomal protein genes rplK and rplA were cotranscribed as a single mRNA of 1.6 kb. A consensus L1 protein binding sequence was identified in the leader region of the rplK-rplA transcript, suggesting that expression of the rplK-rplA cluster was regulated by autogenous regulation exerted by the L1 protein at the translation level. The promoters of the nusG and rplK-rplA genes were subcloned in a novel corynebacterial promoter-probe vector and shown to confer strong expression of the reporter gene.</abstract><cop>Washington, DC</cop><pub>American Society for Microbiology</pub><pmid>11319098</pmid><doi>10.1128/AEM.67.5.2183-2190.2001</doi><tpages>8</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Applied and environmental microbiology, 2001-05, Vol.67 (5), p.2183-2190 |
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| language | eng |
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| source | American Society for Microbiology (ASM) Journals; PubMed Central |
| subjects | Bacteria Bacteriology Base Sequence Biological and medical sciences Blotting, Northern Cloning, Molecular Corynebacterium - genetics Corynebacterium - growth & development Corynebacterium - metabolism Corynebacterium glutamicum Fundamental and applied biological sciences. Psychology Gene Expression Regulation, Bacterial Genes Genes, Bacterial Genetics Genetics and Molecular Biology Genomics Microbiology Molecular Sequence Data Multigene Family nusG gene Operon - genetics pkwR gene Plasmids - genetics Promoter Regions, Genetic - genetics Proteins Ribonucleic acid ribosomal protein L1 ribosomal protein L11 Ribosomal Proteins - genetics Ribosomal Proteins - metabolism RNA rplA gene rplK gene secE gene Sequence Analysis, DNA Transcription, Genetic tRNA Trp |
| title | Organization and transcriptional analysis of a six-gene cluster around the rplK-rplA operon of Corynebacterium glutamicum encoding the ribosomal proteins L11 and L1 |
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