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Cadmium specific proteomic responses of a highly resistant Pseudomonas aeruginosa san ai
san ai is a promising candidate for bioremediation of cadmium pollution, as it resists a high concentration of up to 7.2 mM of cadmium. Leaving biomass of san ai exposed to cadmium has a large biosorption potential, implying its capacity to extract heavy metal from contaminated medium. In the presen...
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| Published in: | RSC advances 2018-01, Vol.8 (19), p.10549-10560 |
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| container_title | RSC advances |
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| creator | Izrael-Živković, Lidija Rikalović, Milena Gojgić-Cvijović, Gordana Kazazić, Saša Vrvić, Miroslav Brčeski, Ilija Beškoski, Vladimir Lončarević, Branka Gopčević, Kristina Karadžić, Ivanka |
| description | san ai is a promising candidate for bioremediation of cadmium pollution, as it resists a high concentration of up to 7.2 mM of cadmium. Leaving biomass of
san ai exposed to cadmium has a large biosorption potential, implying its capacity to extract heavy metal from contaminated medium. In the present study, we investigated tolerance and accumulation of cadmium on protein level by shotgun proteomics approach based on liquid chromatography and tandem mass spectrometry coupled with bioinformatics to identify proteins. Size exclusion chromatography was used for protein prefractionation to preserve native forms of metalloproteins and protein complexes. Using this approach a total of 60 proteins were observed as up-regulated in cadmium-amended culture. Almost a third of the total numbers of up-regulated were metalloproteins. Particularly interesting are denitrification proteins which are over expressed but not active, suggesting their protective role in conditions of heavy metal exposure.
san ai developed a complex mechanism to adapt to cadmium, based on: extracellular biosorption, bioaccumulation, the formation of biofilm, controlled siderophore production, enhanced respiration and modified protein profile. An increased abundance of proteins involved in: cell energy metabolism, including denitrification proteins; amino acid metabolism; cell motility and posttranslational modifications, primarily based on thiol-disulfide exchange, were observed. Enhanced oxygen consumption of biomass in cadmium-amended culture
control was found. Our results signify that
san ai is naturally well equipped to overcome and survive high doses of cadmium and, as such, has a great potential for application in bioremediation of cadmium polluted sites. |
| doi_str_mv | 10.1039/c8ra00371h |
| format | article |
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san ai exposed to cadmium has a large biosorption potential, implying its capacity to extract heavy metal from contaminated medium. In the present study, we investigated tolerance and accumulation of cadmium on protein level by shotgun proteomics approach based on liquid chromatography and tandem mass spectrometry coupled with bioinformatics to identify proteins. Size exclusion chromatography was used for protein prefractionation to preserve native forms of metalloproteins and protein complexes. Using this approach a total of 60 proteins were observed as up-regulated in cadmium-amended culture. Almost a third of the total numbers of up-regulated were metalloproteins. Particularly interesting are denitrification proteins which are over expressed but not active, suggesting their protective role in conditions of heavy metal exposure.
san ai developed a complex mechanism to adapt to cadmium, based on: extracellular biosorption, bioaccumulation, the formation of biofilm, controlled siderophore production, enhanced respiration and modified protein profile. An increased abundance of proteins involved in: cell energy metabolism, including denitrification proteins; amino acid metabolism; cell motility and posttranslational modifications, primarily based on thiol-disulfide exchange, were observed. Enhanced oxygen consumption of biomass in cadmium-amended culture
control was found. Our results signify that
san ai is naturally well equipped to overcome and survive high doses of cadmium and, as such, has a great potential for application in bioremediation of cadmium polluted sites.</description><identifier>ISSN: 2046-2069</identifier><identifier>EISSN: 2046-2069</identifier><identifier>DOI: 10.1039/c8ra00371h</identifier><identifier>PMID: 35540485</identifier><language>eng</language><publisher>England: Royal Society of Chemistry</publisher><subject>Bioaccumulation ; Bioinformatics ; Biomass ; Bioremediation ; Cadmium ; Chemistry ; Chromatography ; Heavy metals ; Mass spectrometry ; Metabolism ; Oxygen consumption ; Proteins ; Proteomics ; Pseudomonas aeruginosa ; Resists ; Size exclusion chromatography</subject><ispartof>RSC advances, 2018-01, Vol.8 (19), p.10549-10560</ispartof><rights>This journal is © The Royal Society of Chemistry.</rights><rights>Copyright Royal Society of Chemistry 2018</rights><rights>This journal is © The Royal Society of Chemistry 2018 The Royal Society of Chemistry</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c411t-cb5b297868debaa32a331ba06fa472382c60af3add498afcdfcc0714fa940d413</citedby><cites>FETCH-LOGICAL-c411t-cb5b297868debaa32a331ba06fa472382c60af3add498afcdfcc0714fa940d413</cites><orcidid>0000-0002-0385-4373 ; 0000-0003-4694-7972 ; 0000-0002-6185-2229</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC9078880/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC9078880/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,315,733,786,790,891,27957,27958,53827,53829</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/35540485$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Izrael-Živković, Lidija</creatorcontrib><creatorcontrib>Rikalović, Milena</creatorcontrib><creatorcontrib>Gojgić-Cvijović, Gordana</creatorcontrib><creatorcontrib>Kazazić, Saša</creatorcontrib><creatorcontrib>Vrvić, Miroslav</creatorcontrib><creatorcontrib>Brčeski, Ilija</creatorcontrib><creatorcontrib>Beškoski, Vladimir</creatorcontrib><creatorcontrib>Lončarević, Branka</creatorcontrib><creatorcontrib>Gopčević, Kristina</creatorcontrib><creatorcontrib>Karadžić, Ivanka</creatorcontrib><title>Cadmium specific proteomic responses of a highly resistant Pseudomonas aeruginosa san ai</title><title>RSC advances</title><addtitle>RSC Adv</addtitle><description>san ai is a promising candidate for bioremediation of cadmium pollution, as it resists a high concentration of up to 7.2 mM of cadmium. Leaving biomass of
san ai exposed to cadmium has a large biosorption potential, implying its capacity to extract heavy metal from contaminated medium. In the present study, we investigated tolerance and accumulation of cadmium on protein level by shotgun proteomics approach based on liquid chromatography and tandem mass spectrometry coupled with bioinformatics to identify proteins. Size exclusion chromatography was used for protein prefractionation to preserve native forms of metalloproteins and protein complexes. Using this approach a total of 60 proteins were observed as up-regulated in cadmium-amended culture. Almost a third of the total numbers of up-regulated were metalloproteins. Particularly interesting are denitrification proteins which are over expressed but not active, suggesting their protective role in conditions of heavy metal exposure.
san ai developed a complex mechanism to adapt to cadmium, based on: extracellular biosorption, bioaccumulation, the formation of biofilm, controlled siderophore production, enhanced respiration and modified protein profile. An increased abundance of proteins involved in: cell energy metabolism, including denitrification proteins; amino acid metabolism; cell motility and posttranslational modifications, primarily based on thiol-disulfide exchange, were observed. Enhanced oxygen consumption of biomass in cadmium-amended culture
control was found. Our results signify that
san ai is naturally well equipped to overcome and survive high doses of cadmium and, as such, has a great potential for application in bioremediation of cadmium polluted sites.</description><subject>Bioaccumulation</subject><subject>Bioinformatics</subject><subject>Biomass</subject><subject>Bioremediation</subject><subject>Cadmium</subject><subject>Chemistry</subject><subject>Chromatography</subject><subject>Heavy metals</subject><subject>Mass spectrometry</subject><subject>Metabolism</subject><subject>Oxygen consumption</subject><subject>Proteins</subject><subject>Proteomics</subject><subject>Pseudomonas aeruginosa</subject><subject>Resists</subject><subject>Size exclusion chromatography</subject><issn>2046-2069</issn><issn>2046-2069</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2018</creationdate><recordtype>article</recordtype><recordid>eNpdkV9rFTEQxYMottS--AEk4IsI1-bfZpMXoVzUCoWWouBbmM0m96bsJmtmV-i3d6-tpTovM8z8OJzhEPKasw-cSXvmTQXGZMv3z8ixYEpvBNP2-ZP5iJwi3rK1dMOF5i_JkWwaxZRpjsmPLfRjWkaKU_ApJk-nWuZQxnWqAaeSMSAtkQLdp91-uDtsE86QZ3qNYenLWDIghVCXXcoFgSJkCukVeRFhwHD60E_I98-fvm0vNpdXX75uzy83XnE-b3zXdMK2Rps-dABSgJS8A6YjqFZII7xmECX0vbIGou-j96zlKoJVrFdcnpCP97rT0o2h9yHPFQY31TRCvXMFkvv3ktPe7covZ1lrjGGrwLsHgVp-LgFnNyb0YRggh7KgE1qLRrWtalf07X_obVlqXt9zgnFltbXm4Oj9PeVrQawhPprhzB0yc1tzc_4ns4sVfvPU_iP6NyH5G2Mkk5A</recordid><startdate>20180101</startdate><enddate>20180101</enddate><creator>Izrael-Živković, Lidija</creator><creator>Rikalović, Milena</creator><creator>Gojgić-Cvijović, Gordana</creator><creator>Kazazić, Saša</creator><creator>Vrvić, Miroslav</creator><creator>Brčeski, Ilija</creator><creator>Beškoski, Vladimir</creator><creator>Lončarević, Branka</creator><creator>Gopčević, Kristina</creator><creator>Karadžić, Ivanka</creator><general>Royal Society of Chemistry</general><general>The Royal Society of Chemistry</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7SR</scope><scope>8BQ</scope><scope>8FD</scope><scope>JG9</scope><scope>7X8</scope><scope>5PM</scope><orcidid>https://orcid.org/0000-0002-0385-4373</orcidid><orcidid>https://orcid.org/0000-0003-4694-7972</orcidid><orcidid>https://orcid.org/0000-0002-6185-2229</orcidid></search><sort><creationdate>20180101</creationdate><title>Cadmium specific proteomic responses of a highly resistant Pseudomonas aeruginosa san ai</title><author>Izrael-Živković, Lidija ; Rikalović, Milena ; Gojgić-Cvijović, Gordana ; Kazazić, Saša ; Vrvić, Miroslav ; Brčeski, Ilija ; Beškoski, Vladimir ; Lončarević, Branka ; Gopčević, Kristina ; Karadžić, Ivanka</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c411t-cb5b297868debaa32a331ba06fa472382c60af3add498afcdfcc0714fa940d413</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2018</creationdate><topic>Bioaccumulation</topic><topic>Bioinformatics</topic><topic>Biomass</topic><topic>Bioremediation</topic><topic>Cadmium</topic><topic>Chemistry</topic><topic>Chromatography</topic><topic>Heavy metals</topic><topic>Mass spectrometry</topic><topic>Metabolism</topic><topic>Oxygen consumption</topic><topic>Proteins</topic><topic>Proteomics</topic><topic>Pseudomonas aeruginosa</topic><topic>Resists</topic><topic>Size exclusion chromatography</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Izrael-Živković, Lidija</creatorcontrib><creatorcontrib>Rikalović, Milena</creatorcontrib><creatorcontrib>Gojgić-Cvijović, Gordana</creatorcontrib><creatorcontrib>Kazazić, Saša</creatorcontrib><creatorcontrib>Vrvić, Miroslav</creatorcontrib><creatorcontrib>Brčeski, Ilija</creatorcontrib><creatorcontrib>Beškoski, Vladimir</creatorcontrib><creatorcontrib>Lončarević, Branka</creatorcontrib><creatorcontrib>Gopčević, Kristina</creatorcontrib><creatorcontrib>Karadžić, Ivanka</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>Engineered Materials Abstracts</collection><collection>METADEX</collection><collection>Technology Research Database</collection><collection>Materials Research Database</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>RSC advances</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Izrael-Živković, Lidija</au><au>Rikalović, Milena</au><au>Gojgić-Cvijović, Gordana</au><au>Kazazić, Saša</au><au>Vrvić, Miroslav</au><au>Brčeski, Ilija</au><au>Beškoski, Vladimir</au><au>Lončarević, Branka</au><au>Gopčević, Kristina</au><au>Karadžić, Ivanka</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Cadmium specific proteomic responses of a highly resistant Pseudomonas aeruginosa san ai</atitle><jtitle>RSC advances</jtitle><addtitle>RSC Adv</addtitle><date>2018-01-01</date><risdate>2018</risdate><volume>8</volume><issue>19</issue><spage>10549</spage><epage>10560</epage><pages>10549-10560</pages><issn>2046-2069</issn><eissn>2046-2069</eissn><notes>ObjectType-Article-1</notes><notes>SourceType-Scholarly Journals-1</notes><notes>ObjectType-Feature-2</notes><notes>content type line 23</notes><notes>Authors equally contributed.</notes><abstract>san ai is a promising candidate for bioremediation of cadmium pollution, as it resists a high concentration of up to 7.2 mM of cadmium. Leaving biomass of
san ai exposed to cadmium has a large biosorption potential, implying its capacity to extract heavy metal from contaminated medium. In the present study, we investigated tolerance and accumulation of cadmium on protein level by shotgun proteomics approach based on liquid chromatography and tandem mass spectrometry coupled with bioinformatics to identify proteins. Size exclusion chromatography was used for protein prefractionation to preserve native forms of metalloproteins and protein complexes. Using this approach a total of 60 proteins were observed as up-regulated in cadmium-amended culture. Almost a third of the total numbers of up-regulated were metalloproteins. Particularly interesting are denitrification proteins which are over expressed but not active, suggesting their protective role in conditions of heavy metal exposure.
san ai developed a complex mechanism to adapt to cadmium, based on: extracellular biosorption, bioaccumulation, the formation of biofilm, controlled siderophore production, enhanced respiration and modified protein profile. An increased abundance of proteins involved in: cell energy metabolism, including denitrification proteins; amino acid metabolism; cell motility and posttranslational modifications, primarily based on thiol-disulfide exchange, were observed. Enhanced oxygen consumption of biomass in cadmium-amended culture
control was found. Our results signify that
san ai is naturally well equipped to overcome and survive high doses of cadmium and, as such, has a great potential for application in bioremediation of cadmium polluted sites.</abstract><cop>England</cop><pub>Royal Society of Chemistry</pub><pmid>35540485</pmid><doi>10.1039/c8ra00371h</doi><tpages>12</tpages><orcidid>https://orcid.org/0000-0002-0385-4373</orcidid><orcidid>https://orcid.org/0000-0003-4694-7972</orcidid><orcidid>https://orcid.org/0000-0002-6185-2229</orcidid><oa>free_for_read</oa></addata></record> |
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| subjects | Bioaccumulation Bioinformatics Biomass Bioremediation Cadmium Chemistry Chromatography Heavy metals Mass spectrometry Metabolism Oxygen consumption Proteins Proteomics Pseudomonas aeruginosa Resists Size exclusion chromatography |
| title | Cadmium specific proteomic responses of a highly resistant Pseudomonas aeruginosa san ai |
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