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Metagenomic and Metatranscriptomic Responses of Chemical Dispersant Application during a Marine Dilbit Spill
The global increase in marine transportation of dilbit (diluted bitumen) can increase the risk of spills, and the application of chemical dispersants remains a common response practice in spill events. To reliably evaluate dispersant effects on dilbit biodegradation over time, we set large-scale (1,...
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| Published in: | Applied and environmental microbiology 2022-03, Vol.88 (5), p.e0215121-e0215121 |
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| container_title | Applied and environmental microbiology |
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| creator | Cao, Yiqi Zhang, Baiyu Greer, Charles W Lee, Kenneth Cai, Qinhong Song, Xing Tremblay, Julien Zhu, Zhiwen Dong, Guihua Chen, Bing |
| description | The global increase in marine transportation of dilbit (diluted bitumen) can increase the risk of spills, and the application of chemical dispersants remains a common response practice in spill events. To reliably evaluate dispersant effects on dilbit biodegradation over time, we set large-scale (1,500 mL) microcosms without nutrient addition using a low dilbit concentration (30 ppm). Shotgun metagenomics and metatranscriptomics were deployed to investigate microbial community responses to naturally and chemically dispersed dilbit. We found that the large-scale microcosms could produce more reproducible community trajectories than small-scale (250 mL) ones based on the 16S rRNA gene amplicon sequencing. In the early-stage large-scale microcosms, multiple genera were involved in the biodegradation of dilbit, while dispersant addition enriched primarily
and competed for the utilization of dilbit, causing depressed degradation of aromatics. The metatranscriptomic-based metagenome-assembled genomes (MAG) further elucidated early-stage microbial antioxidation mechanism, which showed that dispersant addition triggered the increased expression of the antioxidation process genes of
species. Differently, in the late stage, the microbial communities showed high diversity and richness and similar compositions and metabolic functions regardless of dispersant addition, indicating that the biotransformation of remaining compounds can occur within the post-oil communities. These findings can guide future microcosm studies and the application of chemical dispersants for responding to a marine dilbit spill.
In this study, we employed microcosms to study the effects of marine dilbit spill and dispersant application on microbial community dynamics over time. We evaluated the impacts of microcosm scale and found that increasing the scale is beneficial for reducing community stochasticity, especially in the late stage of biodegradation. We observed that dispersant application suppressed aromatics biodegradation in the early stage (6 days), whereas exerting insignificant effects in the late stage (50 days), from both substance removal and metagenomic/metatranscriptomic perspectives. We further found that
species are vital for the early-stage chemically dispersed oil biodegradation and clarified their degradation and antioxidation mechanisms. These findings help us to better understand microcosm studies and microbial roles for biodegrading dilbit and chemically dispersed dilbit |
| doi_str_mv | 10.1128/aem.02151-21 |
| format | article |
| fullrecord | <record><control><sourceid>proquest_pubme</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_8904047</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2619545813</sourcerecordid><originalsourceid>FETCH-LOGICAL-a446t-72d5cafea336d5a97b26d28ef782017a668591d0897ff1fa299f244727e07fcb3</originalsourceid><addsrcrecordid>eNp1kc1rFTEUxYMo9rW6cy0BNxacmmSSSbIRylNroUXwYx3um0leUzLJmMwI_vfm9dVWC65yc-6PQ04OQi8oOaGUqbdgxxPCqKANo4_QihKtGtG23WO0IkTrhjFODtBhKdeEEE469RQdtIIwwoVYoXBpZ9jamEbfY4gD3t3nDLH02U_zjfzFlinFYgtODq-vbNUg4Pe-TDYXiDM-naZQtdmniIcl-7jFgC-hDrZiYeNn_HXyITxDTxyEYp_fnkfo-8cP39afmovPZ-fr04sGOO_mRrJB9OAs1BSDAC03rBuYsk4qRqiErlNC04EoLZ2jDpjWjnEumbREun7THqF3e99p2Yx26G2siYKZsh8h_zIJvPl3E_2V2aafRun6Q1xWg9e3Bjn9WGyZzehLb0OAaNNSDOuoFlwo2lb01QP0Oi051niV4oSSSu6oN3uqz6mUbN3dYygxuxpNrdHc1GgYrfjxHocysnvD_7Av_w57Z_yn4_Y3X2Km4Q</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2640101953</pqid></control><display><type>article</type><title>Metagenomic and Metatranscriptomic Responses of Chemical Dispersant Application during a Marine Dilbit Spill</title><source>American Society for Microbiology Journals</source><source>PubMed Central</source><creator>Cao, Yiqi ; Zhang, Baiyu ; Greer, Charles W ; Lee, Kenneth ; Cai, Qinhong ; Song, Xing ; Tremblay, Julien ; Zhu, Zhiwen ; Dong, Guihua ; Chen, Bing</creator><contributor>Nojiri, Hideaki</contributor><creatorcontrib>Cao, Yiqi ; Zhang, Baiyu ; Greer, Charles W ; Lee, Kenneth ; Cai, Qinhong ; Song, Xing ; Tremblay, Julien ; Zhu, Zhiwen ; Dong, Guihua ; Chen, Bing ; Nojiri, Hideaki</creatorcontrib><description>The global increase in marine transportation of dilbit (diluted bitumen) can increase the risk of spills, and the application of chemical dispersants remains a common response practice in spill events. To reliably evaluate dispersant effects on dilbit biodegradation over time, we set large-scale (1,500 mL) microcosms without nutrient addition using a low dilbit concentration (30 ppm). Shotgun metagenomics and metatranscriptomics were deployed to investigate microbial community responses to naturally and chemically dispersed dilbit. We found that the large-scale microcosms could produce more reproducible community trajectories than small-scale (250 mL) ones based on the 16S rRNA gene amplicon sequencing. In the early-stage large-scale microcosms, multiple genera were involved in the biodegradation of dilbit, while dispersant addition enriched primarily
and competed for the utilization of dilbit, causing depressed degradation of aromatics. The metatranscriptomic-based metagenome-assembled genomes (MAG) further elucidated early-stage microbial antioxidation mechanism, which showed that dispersant addition triggered the increased expression of the antioxidation process genes of
species. Differently, in the late stage, the microbial communities showed high diversity and richness and similar compositions and metabolic functions regardless of dispersant addition, indicating that the biotransformation of remaining compounds can occur within the post-oil communities. These findings can guide future microcosm studies and the application of chemical dispersants for responding to a marine dilbit spill.
In this study, we employed microcosms to study the effects of marine dilbit spill and dispersant application on microbial community dynamics over time. We evaluated the impacts of microcosm scale and found that increasing the scale is beneficial for reducing community stochasticity, especially in the late stage of biodegradation. We observed that dispersant application suppressed aromatics biodegradation in the early stage (6 days), whereas exerting insignificant effects in the late stage (50 days), from both substance removal and metagenomic/metatranscriptomic perspectives. We further found that
species are vital for the early-stage chemically dispersed oil biodegradation and clarified their degradation and antioxidation mechanisms. These findings help us to better understand microcosm studies and microbial roles for biodegrading dilbit and chemically dispersed dilbit and suggest that dispersant evaluation in large-scale systems and even through field trails would be more realistic after marine oil spill response.</description><identifier>ISSN: 0099-2240</identifier><identifier>EISSN: 1098-5336</identifier><identifier>DOI: 10.1128/aem.02151-21</identifier><identifier>PMID: 35020455</identifier><language>eng</language><publisher>United States: American Society for Microbiology</publisher><subject>Alteromonas ; Aromatic compounds ; Biodegradation ; Biodegradation, Environmental ; Biotransformation ; Dispersants ; Dispersion ; Environmental Microbiology ; Gene sequencing ; Genomes ; Marine transportation ; Metagenome ; Metagenomics ; Microbial activity ; Microcosms ; Microorganisms ; Nutrient concentrations ; Oil spills ; Petroleum - metabolism ; Petroleum Pollution - analysis ; RNA, Ribosomal, 16S - genetics ; rRNA 16S ; Seawater - chemistry ; Stochasticity ; Systems analysis ; Water Pollutants, Chemical - analysis</subject><ispartof>Applied and environmental microbiology, 2022-03, Vol.88 (5), p.e0215121-e0215121</ispartof><rights>Copyright © 2022 American Society for Microbiology.</rights><rights>Copyright American Society for Microbiology Mar 2022</rights><rights>Copyright © 2022 American Society for Microbiology. 2022 American Society for Microbiology</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-a446t-72d5cafea336d5a97b26d28ef782017a668591d0897ff1fa299f244727e07fcb3</citedby><cites>FETCH-LOGICAL-a446t-72d5cafea336d5a97b26d28ef782017a668591d0897ff1fa299f244727e07fcb3</cites><orcidid>0000-0002-6997-4765</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://journals.asm.org/doi/pdf/10.1128/aem.02151-21$$EPDF$$P50$$Gasm2$$H</linktopdf><linktohtml>$$Uhttps://journals.asm.org/doi/full/10.1128/aem.02151-21$$EHTML$$P50$$Gasm2$$H</linktohtml><link.rule.ids>230,315,733,786,790,891,3207,27957,27958,52786,52787,52788,53827,53829</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/35020455$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>Nojiri, Hideaki</contributor><creatorcontrib>Cao, Yiqi</creatorcontrib><creatorcontrib>Zhang, Baiyu</creatorcontrib><creatorcontrib>Greer, Charles W</creatorcontrib><creatorcontrib>Lee, Kenneth</creatorcontrib><creatorcontrib>Cai, Qinhong</creatorcontrib><creatorcontrib>Song, Xing</creatorcontrib><creatorcontrib>Tremblay, Julien</creatorcontrib><creatorcontrib>Zhu, Zhiwen</creatorcontrib><creatorcontrib>Dong, Guihua</creatorcontrib><creatorcontrib>Chen, Bing</creatorcontrib><title>Metagenomic and Metatranscriptomic Responses of Chemical Dispersant Application during a Marine Dilbit Spill</title><title>Applied and environmental microbiology</title><addtitle>Appl Environ Microbiol</addtitle><addtitle>Appl Environ Microbiol</addtitle><description>The global increase in marine transportation of dilbit (diluted bitumen) can increase the risk of spills, and the application of chemical dispersants remains a common response practice in spill events. To reliably evaluate dispersant effects on dilbit biodegradation over time, we set large-scale (1,500 mL) microcosms without nutrient addition using a low dilbit concentration (30 ppm). Shotgun metagenomics and metatranscriptomics were deployed to investigate microbial community responses to naturally and chemically dispersed dilbit. We found that the large-scale microcosms could produce more reproducible community trajectories than small-scale (250 mL) ones based on the 16S rRNA gene amplicon sequencing. In the early-stage large-scale microcosms, multiple genera were involved in the biodegradation of dilbit, while dispersant addition enriched primarily
and competed for the utilization of dilbit, causing depressed degradation of aromatics. The metatranscriptomic-based metagenome-assembled genomes (MAG) further elucidated early-stage microbial antioxidation mechanism, which showed that dispersant addition triggered the increased expression of the antioxidation process genes of
species. Differently, in the late stage, the microbial communities showed high diversity and richness and similar compositions and metabolic functions regardless of dispersant addition, indicating that the biotransformation of remaining compounds can occur within the post-oil communities. These findings can guide future microcosm studies and the application of chemical dispersants for responding to a marine dilbit spill.
In this study, we employed microcosms to study the effects of marine dilbit spill and dispersant application on microbial community dynamics over time. We evaluated the impacts of microcosm scale and found that increasing the scale is beneficial for reducing community stochasticity, especially in the late stage of biodegradation. We observed that dispersant application suppressed aromatics biodegradation in the early stage (6 days), whereas exerting insignificant effects in the late stage (50 days), from both substance removal and metagenomic/metatranscriptomic perspectives. We further found that
species are vital for the early-stage chemically dispersed oil biodegradation and clarified their degradation and antioxidation mechanisms. These findings help us to better understand microcosm studies and microbial roles for biodegrading dilbit and chemically dispersed dilbit and suggest that dispersant evaluation in large-scale systems and even through field trails would be more realistic after marine oil spill response.</description><subject>Alteromonas</subject><subject>Aromatic compounds</subject><subject>Biodegradation</subject><subject>Biodegradation, Environmental</subject><subject>Biotransformation</subject><subject>Dispersants</subject><subject>Dispersion</subject><subject>Environmental Microbiology</subject><subject>Gene sequencing</subject><subject>Genomes</subject><subject>Marine transportation</subject><subject>Metagenome</subject><subject>Metagenomics</subject><subject>Microbial activity</subject><subject>Microcosms</subject><subject>Microorganisms</subject><subject>Nutrient concentrations</subject><subject>Oil spills</subject><subject>Petroleum - metabolism</subject><subject>Petroleum Pollution - analysis</subject><subject>RNA, Ribosomal, 16S - genetics</subject><subject>rRNA 16S</subject><subject>Seawater - chemistry</subject><subject>Stochasticity</subject><subject>Systems analysis</subject><subject>Water Pollutants, Chemical - analysis</subject><issn>0099-2240</issn><issn>1098-5336</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2022</creationdate><recordtype>article</recordtype><recordid>eNp1kc1rFTEUxYMo9rW6cy0BNxacmmSSSbIRylNroUXwYx3um0leUzLJmMwI_vfm9dVWC65yc-6PQ04OQi8oOaGUqbdgxxPCqKANo4_QihKtGtG23WO0IkTrhjFODtBhKdeEEE469RQdtIIwwoVYoXBpZ9jamEbfY4gD3t3nDLH02U_zjfzFlinFYgtODq-vbNUg4Pe-TDYXiDM-naZQtdmniIcl-7jFgC-hDrZiYeNn_HXyITxDTxyEYp_fnkfo-8cP39afmovPZ-fr04sGOO_mRrJB9OAs1BSDAC03rBuYsk4qRqiErlNC04EoLZ2jDpjWjnEumbREun7THqF3e99p2Yx26G2siYKZsh8h_zIJvPl3E_2V2aafRun6Q1xWg9e3Bjn9WGyZzehLb0OAaNNSDOuoFlwo2lb01QP0Oi051niV4oSSSu6oN3uqz6mUbN3dYygxuxpNrdHc1GgYrfjxHocysnvD_7Av_w57Z_yn4_Y3X2Km4Q</recordid><startdate>20220308</startdate><enddate>20220308</enddate><creator>Cao, Yiqi</creator><creator>Zhang, Baiyu</creator><creator>Greer, Charles W</creator><creator>Lee, Kenneth</creator><creator>Cai, Qinhong</creator><creator>Song, Xing</creator><creator>Tremblay, Julien</creator><creator>Zhu, Zhiwen</creator><creator>Dong, Guihua</creator><creator>Chen, Bing</creator><general>American Society for Microbiology</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7QO</scope><scope>7SN</scope><scope>7SS</scope><scope>7ST</scope><scope>7T7</scope><scope>7TM</scope><scope>7U9</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope><scope>SOI</scope><scope>7X8</scope><scope>5PM</scope><orcidid>https://orcid.org/0000-0002-6997-4765</orcidid></search><sort><creationdate>20220308</creationdate><title>Metagenomic and Metatranscriptomic Responses of Chemical Dispersant Application during a Marine Dilbit Spill</title><author>Cao, Yiqi ; 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To reliably evaluate dispersant effects on dilbit biodegradation over time, we set large-scale (1,500 mL) microcosms without nutrient addition using a low dilbit concentration (30 ppm). Shotgun metagenomics and metatranscriptomics were deployed to investigate microbial community responses to naturally and chemically dispersed dilbit. We found that the large-scale microcosms could produce more reproducible community trajectories than small-scale (250 mL) ones based on the 16S rRNA gene amplicon sequencing. In the early-stage large-scale microcosms, multiple genera were involved in the biodegradation of dilbit, while dispersant addition enriched primarily
and competed for the utilization of dilbit, causing depressed degradation of aromatics. The metatranscriptomic-based metagenome-assembled genomes (MAG) further elucidated early-stage microbial antioxidation mechanism, which showed that dispersant addition triggered the increased expression of the antioxidation process genes of
species. Differently, in the late stage, the microbial communities showed high diversity and richness and similar compositions and metabolic functions regardless of dispersant addition, indicating that the biotransformation of remaining compounds can occur within the post-oil communities. These findings can guide future microcosm studies and the application of chemical dispersants for responding to a marine dilbit spill.
In this study, we employed microcosms to study the effects of marine dilbit spill and dispersant application on microbial community dynamics over time. We evaluated the impacts of microcosm scale and found that increasing the scale is beneficial for reducing community stochasticity, especially in the late stage of biodegradation. We observed that dispersant application suppressed aromatics biodegradation in the early stage (6 days), whereas exerting insignificant effects in the late stage (50 days), from both substance removal and metagenomic/metatranscriptomic perspectives. We further found that
species are vital for the early-stage chemically dispersed oil biodegradation and clarified their degradation and antioxidation mechanisms. These findings help us to better understand microcosm studies and microbial roles for biodegrading dilbit and chemically dispersed dilbit and suggest that dispersant evaluation in large-scale systems and even through field trails would be more realistic after marine oil spill response.</abstract><cop>United States</cop><pub>American Society for Microbiology</pub><pmid>35020455</pmid><doi>10.1128/aem.02151-21</doi><tpages>16</tpages><orcidid>https://orcid.org/0000-0002-6997-4765</orcidid><oa>free_for_read</oa></addata></record> |
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| source | American Society for Microbiology Journals; PubMed Central |
| subjects | Alteromonas Aromatic compounds Biodegradation Biodegradation, Environmental Biotransformation Dispersants Dispersion Environmental Microbiology Gene sequencing Genomes Marine transportation Metagenome Metagenomics Microbial activity Microcosms Microorganisms Nutrient concentrations Oil spills Petroleum - metabolism Petroleum Pollution - analysis RNA, Ribosomal, 16S - genetics rRNA 16S Seawater - chemistry Stochasticity Systems analysis Water Pollutants, Chemical - analysis |
| title | Metagenomic and Metatranscriptomic Responses of Chemical Dispersant Application during a Marine Dilbit Spill |
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