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Conformational Tuning of Amylin by Charged Styrene-Maleic-Acid Copolymers
[Display omitted] •The generation of morphologically and pathologically distinct amylin species is reported.•Cationic SMAQA polymer drives the formation of amylin globulomer intermediates.•Anionic SMAEA accelerates amyloid formation by suppressing the intermediates.•Amylin species formed with the co...
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| Published in: | Journal of molecular biology 2022-01, Vol.434 (2), p.167385-167385, Article 167385 |
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| creator | Sahoo, Bikash R. Souders, Christopher L. Watanabe-Nakayama, Takahiro Deng, Zhou Linton, Hunter Suladze, Saba Ivanova, Magdalena I. Reif, Bernd Ando, Toshio Martyniuk, Christopher J. Ramamoorthy, Ayyalusamy |
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•The generation of morphologically and pathologically distinct amylin species is reported.•Cationic SMAQA polymer drives the formation of amylin globulomer intermediates.•Anionic SMAEA accelerates amyloid formation by suppressing the intermediates.•Amylin species formed with the copolymers showed minimal deformity to zebrafish embryos.
Human amylin forms structurally heterogeneous amyloids that have been linked to type-2 diabetes. Thus, understanding the molecular interactions governing amylin aggregation can provide mechanistic insights in its pathogenic formation. Here, we demonstrate that fibril formation of amylin is altered by synthetic amphipathic copolymer derivatives of the styrene-maleic-acid (SMAQA and SMAEA). High-speed AFM is used to follow the real-time aggregation of amylin by observing the rapid formation of de novo globular oligomers and arrestment of fibrillation by the positively-charged SMAQA. We also observed an accelerated fibril formation in the presence of the negatively-charged SMAEA. These findings were further validated by fluorescence, SOFAST-HMQC, DOSY and STD NMR experiments. Conformational analysis by CD and FT-IR revealed that the SMA copolymers modulate the conformation of amylin aggregates. While the species formed with SMAQA are α-helical, the ones formed with SMAEA are rich in β-sheet structure. The interacting interfaces between SMAEA or SMAQA and amylin are mapped by NMR and microseconds all-atom MD simulation. SMAEA displayed π-π interaction with Phe23, electrostatic π-cation interaction with His18 and hydrophobic packing with Ala13 and Val17; whereas SMAQA showed a selective interaction with amylin’s C terminus (residues 31–37) that belongs to one of the two β-sheet regions (residues 14–19 and 31–36) involved in amylin fibrillation. Toxicity analysis showed both SMA copolymers to be non-toxic in vitro and the amylin species formed with the copolymers showed minimal deformity to zebrafish embryos. Together, this study demonstrates that chemical tools, such as copolymers, can be used to modulate amylin aggregation, alter the conformation of species. |
| doi_str_mv | 10.1016/j.jmb.2021.167385 |
| format | article |
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•The generation of morphologically and pathologically distinct amylin species is reported.•Cationic SMAQA polymer drives the formation of amylin globulomer intermediates.•Anionic SMAEA accelerates amyloid formation by suppressing the intermediates.•Amylin species formed with the copolymers showed minimal deformity to zebrafish embryos.
Human amylin forms structurally heterogeneous amyloids that have been linked to type-2 diabetes. Thus, understanding the molecular interactions governing amylin aggregation can provide mechanistic insights in its pathogenic formation. Here, we demonstrate that fibril formation of amylin is altered by synthetic amphipathic copolymer derivatives of the styrene-maleic-acid (SMAQA and SMAEA). High-speed AFM is used to follow the real-time aggregation of amylin by observing the rapid formation of de novo globular oligomers and arrestment of fibrillation by the positively-charged SMAQA. We also observed an accelerated fibril formation in the presence of the negatively-charged SMAEA. These findings were further validated by fluorescence, SOFAST-HMQC, DOSY and STD NMR experiments. Conformational analysis by CD and FT-IR revealed that the SMA copolymers modulate the conformation of amylin aggregates. While the species formed with SMAQA are α-helical, the ones formed with SMAEA are rich in β-sheet structure. The interacting interfaces between SMAEA or SMAQA and amylin are mapped by NMR and microseconds all-atom MD simulation. SMAEA displayed π-π interaction with Phe23, electrostatic π-cation interaction with His18 and hydrophobic packing with Ala13 and Val17; whereas SMAQA showed a selective interaction with amylin’s C terminus (residues 31–37) that belongs to one of the two β-sheet regions (residues 14–19 and 31–36) involved in amylin fibrillation. Toxicity analysis showed both SMA copolymers to be non-toxic in vitro and the amylin species formed with the copolymers showed minimal deformity to zebrafish embryos. Together, this study demonstrates that chemical tools, such as copolymers, can be used to modulate amylin aggregation, alter the conformation of species.</description><identifier>ISSN: 0022-2836</identifier><identifier>EISSN: 1089-8638</identifier><identifier>DOI: 10.1016/j.jmb.2021.167385</identifier><identifier>PMID: 34883118</identifier><language>eng</language><publisher>Netherlands: Elsevier Ltd</publisher><subject>amylin ; amyloid ; Amyloid - chemistry ; Animals ; Computer Simulation ; Diabetes Mellitus, Type 2 ; Fluorescence ; Humans ; Hydrophobic and Hydrophilic Interactions ; IAPP ; Islet Amyloid Polypeptide - chemistry ; Maleates - chemistry ; Molecular Conformation ; Protein Aggregates ; SMA copolymer ; Spectroscopy, Fourier Transform Infrared ; Styrene - chemistry ; Styrenes - chemistry ; type-II diabetes ; Zebrafish</subject><ispartof>Journal of molecular biology, 2022-01, Vol.434 (2), p.167385-167385, Article 167385</ispartof><rights>2021 Elsevier Ltd</rights><rights>Copyright © 2021 Elsevier Ltd. All rights reserved.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c517t-b76aa1701776189e9e749f783cb5c91b4ba09c6122052bf54c5d17c69dfe339d3</citedby><cites>FETCH-LOGICAL-c517t-b76aa1701776189e9e749f783cb5c91b4ba09c6122052bf54c5d17c69dfe339d3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>230,315,786,790,891,27957,27958</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/34883118$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Sahoo, Bikash R.</creatorcontrib><creatorcontrib>Souders, Christopher L.</creatorcontrib><creatorcontrib>Watanabe-Nakayama, Takahiro</creatorcontrib><creatorcontrib>Deng, Zhou</creatorcontrib><creatorcontrib>Linton, Hunter</creatorcontrib><creatorcontrib>Suladze, Saba</creatorcontrib><creatorcontrib>Ivanova, Magdalena I.</creatorcontrib><creatorcontrib>Reif, Bernd</creatorcontrib><creatorcontrib>Ando, Toshio</creatorcontrib><creatorcontrib>Martyniuk, Christopher J.</creatorcontrib><creatorcontrib>Ramamoorthy, Ayyalusamy</creatorcontrib><title>Conformational Tuning of Amylin by Charged Styrene-Maleic-Acid Copolymers</title><title>Journal of molecular biology</title><addtitle>J Mol Biol</addtitle><description>[Display omitted]
•The generation of morphologically and pathologically distinct amylin species is reported.•Cationic SMAQA polymer drives the formation of amylin globulomer intermediates.•Anionic SMAEA accelerates amyloid formation by suppressing the intermediates.•Amylin species formed with the copolymers showed minimal deformity to zebrafish embryos.
Human amylin forms structurally heterogeneous amyloids that have been linked to type-2 diabetes. Thus, understanding the molecular interactions governing amylin aggregation can provide mechanistic insights in its pathogenic formation. Here, we demonstrate that fibril formation of amylin is altered by synthetic amphipathic copolymer derivatives of the styrene-maleic-acid (SMAQA and SMAEA). High-speed AFM is used to follow the real-time aggregation of amylin by observing the rapid formation of de novo globular oligomers and arrestment of fibrillation by the positively-charged SMAQA. We also observed an accelerated fibril formation in the presence of the negatively-charged SMAEA. These findings were further validated by fluorescence, SOFAST-HMQC, DOSY and STD NMR experiments. Conformational analysis by CD and FT-IR revealed that the SMA copolymers modulate the conformation of amylin aggregates. While the species formed with SMAQA are α-helical, the ones formed with SMAEA are rich in β-sheet structure. The interacting interfaces between SMAEA or SMAQA and amylin are mapped by NMR and microseconds all-atom MD simulation. SMAEA displayed π-π interaction with Phe23, electrostatic π-cation interaction with His18 and hydrophobic packing with Ala13 and Val17; whereas SMAQA showed a selective interaction with amylin’s C terminus (residues 31–37) that belongs to one of the two β-sheet regions (residues 14–19 and 31–36) involved in amylin fibrillation. Toxicity analysis showed both SMA copolymers to be non-toxic in vitro and the amylin species formed with the copolymers showed minimal deformity to zebrafish embryos. Together, this study demonstrates that chemical tools, such as copolymers, can be used to modulate amylin aggregation, alter the conformation of species.</description><subject>amylin</subject><subject>amyloid</subject><subject>Amyloid - chemistry</subject><subject>Animals</subject><subject>Computer Simulation</subject><subject>Diabetes Mellitus, Type 2</subject><subject>Fluorescence</subject><subject>Humans</subject><subject>Hydrophobic and Hydrophilic Interactions</subject><subject>IAPP</subject><subject>Islet Amyloid Polypeptide - chemistry</subject><subject>Maleates - chemistry</subject><subject>Molecular Conformation</subject><subject>Protein Aggregates</subject><subject>SMA copolymer</subject><subject>Spectroscopy, Fourier Transform Infrared</subject><subject>Styrene - chemistry</subject><subject>Styrenes - chemistry</subject><subject>type-II diabetes</subject><subject>Zebrafish</subject><issn>0022-2836</issn><issn>1089-8638</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2022</creationdate><recordtype>article</recordtype><recordid>eNp9kNtKxDAQhoMouh4ewBvpC3TNJJtDEYSleALFC_U6pOl0zdImS1qFvr2VVdEbb2YuZv5vmI-QU6BzoCDP1_N1V80ZZTAHqbgWO2QGVBe5llzvkhmljOVMc3lADvt-TSkVfKH3ycFUNQfQM3JXxtDE1NnBx2Db7Pkt-LDKYpMtu7H1IavGrHy1aYV19jSMCQPmD7ZF7_Kl83VWxk1sxw5Tf0z2Gtv2ePLVj8jL9dVzeZvfP97clcv73AlQQ14paS0oCkpJ0AUWqBZFozR3lXAFVIvK0sJJYIwKVjVi4UQNysmibpDzouZH5HLL3bxVHdYOw5BsazbJdzaNJlpv_k6CfzWr-G60EkyAnACwBbgU-z5h85MFaj69mrWZvJpPr2brdcqc_T76k_gWOS1cbBdwev3dYzK98xgc1j6hG0wd_T_4DwEjiYA</recordid><startdate>20220130</startdate><enddate>20220130</enddate><creator>Sahoo, Bikash R.</creator><creator>Souders, Christopher L.</creator><creator>Watanabe-Nakayama, Takahiro</creator><creator>Deng, Zhou</creator><creator>Linton, Hunter</creator><creator>Suladze, Saba</creator><creator>Ivanova, Magdalena I.</creator><creator>Reif, Bernd</creator><creator>Ando, Toshio</creator><creator>Martyniuk, Christopher J.</creator><creator>Ramamoorthy, Ayyalusamy</creator><general>Elsevier Ltd</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>5PM</scope></search><sort><creationdate>20220130</creationdate><title>Conformational Tuning of Amylin by Charged Styrene-Maleic-Acid Copolymers</title><author>Sahoo, Bikash R. ; Souders, Christopher L. ; Watanabe-Nakayama, Takahiro ; Deng, Zhou ; Linton, Hunter ; Suladze, Saba ; Ivanova, Magdalena I. ; Reif, Bernd ; Ando, Toshio ; Martyniuk, Christopher J. ; Ramamoorthy, Ayyalusamy</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c517t-b76aa1701776189e9e749f783cb5c91b4ba09c6122052bf54c5d17c69dfe339d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2022</creationdate><topic>amylin</topic><topic>amyloid</topic><topic>Amyloid - chemistry</topic><topic>Animals</topic><topic>Computer Simulation</topic><topic>Diabetes Mellitus, Type 2</topic><topic>Fluorescence</topic><topic>Humans</topic><topic>Hydrophobic and Hydrophilic Interactions</topic><topic>IAPP</topic><topic>Islet Amyloid Polypeptide - chemistry</topic><topic>Maleates - chemistry</topic><topic>Molecular Conformation</topic><topic>Protein Aggregates</topic><topic>SMA copolymer</topic><topic>Spectroscopy, Fourier Transform Infrared</topic><topic>Styrene - chemistry</topic><topic>Styrenes - chemistry</topic><topic>type-II diabetes</topic><topic>Zebrafish</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Sahoo, Bikash R.</creatorcontrib><creatorcontrib>Souders, Christopher L.</creatorcontrib><creatorcontrib>Watanabe-Nakayama, Takahiro</creatorcontrib><creatorcontrib>Deng, Zhou</creatorcontrib><creatorcontrib>Linton, Hunter</creatorcontrib><creatorcontrib>Suladze, Saba</creatorcontrib><creatorcontrib>Ivanova, Magdalena I.</creatorcontrib><creatorcontrib>Reif, Bernd</creatorcontrib><creatorcontrib>Ando, Toshio</creatorcontrib><creatorcontrib>Martyniuk, Christopher J.</creatorcontrib><creatorcontrib>Ramamoorthy, Ayyalusamy</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Journal of molecular biology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Sahoo, Bikash R.</au><au>Souders, Christopher L.</au><au>Watanabe-Nakayama, Takahiro</au><au>Deng, Zhou</au><au>Linton, Hunter</au><au>Suladze, Saba</au><au>Ivanova, Magdalena I.</au><au>Reif, Bernd</au><au>Ando, Toshio</au><au>Martyniuk, Christopher J.</au><au>Ramamoorthy, Ayyalusamy</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Conformational Tuning of Amylin by Charged Styrene-Maleic-Acid Copolymers</atitle><jtitle>Journal of molecular biology</jtitle><addtitle>J Mol Biol</addtitle><date>2022-01-30</date><risdate>2022</risdate><volume>434</volume><issue>2</issue><spage>167385</spage><epage>167385</epage><pages>167385-167385</pages><artnum>167385</artnum><issn>0022-2836</issn><eissn>1089-8638</eissn><abstract>[Display omitted]
•The generation of morphologically and pathologically distinct amylin species is reported.•Cationic SMAQA polymer drives the formation of amylin globulomer intermediates.•Anionic SMAEA accelerates amyloid formation by suppressing the intermediates.•Amylin species formed with the copolymers showed minimal deformity to zebrafish embryos.
Human amylin forms structurally heterogeneous amyloids that have been linked to type-2 diabetes. Thus, understanding the molecular interactions governing amylin aggregation can provide mechanistic insights in its pathogenic formation. Here, we demonstrate that fibril formation of amylin is altered by synthetic amphipathic copolymer derivatives of the styrene-maleic-acid (SMAQA and SMAEA). High-speed AFM is used to follow the real-time aggregation of amylin by observing the rapid formation of de novo globular oligomers and arrestment of fibrillation by the positively-charged SMAQA. We also observed an accelerated fibril formation in the presence of the negatively-charged SMAEA. These findings were further validated by fluorescence, SOFAST-HMQC, DOSY and STD NMR experiments. Conformational analysis by CD and FT-IR revealed that the SMA copolymers modulate the conformation of amylin aggregates. While the species formed with SMAQA are α-helical, the ones formed with SMAEA are rich in β-sheet structure. The interacting interfaces between SMAEA or SMAQA and amylin are mapped by NMR and microseconds all-atom MD simulation. SMAEA displayed π-π interaction with Phe23, electrostatic π-cation interaction with His18 and hydrophobic packing with Ala13 and Val17; whereas SMAQA showed a selective interaction with amylin’s C terminus (residues 31–37) that belongs to one of the two β-sheet regions (residues 14–19 and 31–36) involved in amylin fibrillation. Toxicity analysis showed both SMA copolymers to be non-toxic in vitro and the amylin species formed with the copolymers showed minimal deformity to zebrafish embryos. Together, this study demonstrates that chemical tools, such as copolymers, can be used to modulate amylin aggregation, alter the conformation of species.</abstract><cop>Netherlands</cop><pub>Elsevier Ltd</pub><pmid>34883118</pmid><doi>10.1016/j.jmb.2021.167385</doi><tpages>1</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | amylin amyloid Amyloid - chemistry Animals Computer Simulation Diabetes Mellitus, Type 2 Fluorescence Humans Hydrophobic and Hydrophilic Interactions IAPP Islet Amyloid Polypeptide - chemistry Maleates - chemistry Molecular Conformation Protein Aggregates SMA copolymer Spectroscopy, Fourier Transform Infrared Styrene - chemistry Styrenes - chemistry type-II diabetes Zebrafish |
| title | Conformational Tuning of Amylin by Charged Styrene-Maleic-Acid Copolymers |
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