A conservative distribution of tridomain NDP-heptose synthetases in actinobacteria
Heptoses are important structural components of Gram-negative bacterium cell wall and participate in bacterial colonization, infection, and immune recognition. Current knowledge of NDP-heptose originating from d -sedoheptulose 7-phosphate in Grampositive bacterium remains limited. Here, in silico an...
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Published in: | Science China. Life sciences 2022-05, Vol.65 (5), p.1014-1023 |
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Main Authors: | , , , , |
Format: | Article |
Language: | eng |
Subjects: | |
Online Access: | Get full text |
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Summary: | Heptoses are important structural components of Gram-negative bacterium cell wall and participate in bacterial colonization, infection, and immune recognition. Current knowledge of NDP-heptose originating from
d
-sedoheptulose 7-phosphate in Grampositive bacterium remains limited. Here,
in silico
analysis suggested that the special tridomain NDP-heptose synthetases with isomerase, kinase, and nucleotidyltransferase activities are conservatively distributed in Actinobacteria class of Gram-positive bacterium. Enzymatical characterization of the tridomain proteins from different strains showed that they are involved in ADP-
d
-
glycero
-
β
-
d
-
manno
-heptose biosynthesis despite the unexpected discovery of kinase activities deficient in some proteins. The presence of three types of NDP-heptose synthetases in Gram-positive bacterium suggests that it is also a rich source of heptoses and the heptose moieties may play important roles
in vivo
. Our work updates the understanding of NDP-heptose biosynthesis in Gram-positive bacterium and lays a solid foundation for further physiological function explorations. |
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ISSN: | 1674-7305 1869-1889 |