Superior japonica rice variety YJ144 with improved rice blast resistance, yield, and quality achieved using molecular design and multiple breeding strategies

Yanfeng 47 (YF47) is an elite japonica rice variety cultivated in China on nearly 2 million hectares over the past 20 years. However, YF47 is highly susceptible to rice blast ( Magnaporthe oryzae ), one of the most destructive rice diseases. In this study, we developed novel TPAP (tetra-primer ARMS-...

Full description

Saved in:
Bibliographic Details
Published in:Molecular breeding 2021-10, Vol.41 (10), p.65-65, Article 65
Main Authors: Mao, Ting, Zhu, Mingdong, Ahmad, Shakeel, Ye, Guoyou, Sheng, Zhonghua, Hu, Shikai, Jiao, Guiai, Xie, Lihong, Tang, Shaoqing, Wei, Xiangjin, Hu, Peisong, Shao, Gaoneng
Format: Article
Language:English
Subjects:
Agricultural production
Alleles
Binding sites
Biomedical and Life Sciences
Biotechnology
Breeding
Cloning
Cultivation
Disease resistance
Epidemics
Fungicides
Genes
Grain cultivation
Interleukin 4
Interleukin 5
Life Sciences
Marker-assisted selection
Markers
Molecular biology
Plant biology
Plant Genetics and Genomics
Plant Pathology
Plant Physiology
Plant reproduction
Plant Sciences
Polymorphism
Proteins
Research centers
Rice
Rice blast
Yield
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Yanfeng 47 (YF47) is an elite japonica rice variety cultivated in China on nearly 2 million hectares over the past 20 years. However, YF47 is highly susceptible to rice blast ( Magnaporthe oryzae ), one of the most destructive rice diseases. In this study, we developed novel TPAP (tetra-primer ARMS-PCR) functional markers for the genes Pita , Pib , and Pid2 , all of which afford broad-spectrum resistance to blast. A collection of 91 japonica rice germplasms with similar ecological characteristics to YF47 were screened, and Wuyunjing 27 (WYJ27) with Pita and Pib alleles and P135 with the Pid2 allele were identified. Furthermore, the corresponding positive Pita , Pib , and Pid2 alleles were transferred into YF47 using single, mutual, and backcrosses, together with molecular marker-assisted selection (MAS) and anther culture technology. These genetic materials, carrying one, two, or three functional alleles, were generated within 3 years, and compared to YF47, they all showed improved resistance to naturally inoculated rice blast. Further improved lines (IL) 1 to 5 (all containing Pita , Pib , and Pid2 alleles) were evaluated for yield performance, and when no fungicide was applied, all lines except IL-4 showed increased traits compared with those of YF47. IL-5, renamed Yanjing 144 (YJ144), showed yield increases in the Liaoning province regional variety comparison test and superior appearance quality compared to YF47. Our work provides a molecular design strategy for pyramiding multiple beneficial genes to rapidly improve rice blast resistance, yield, and quality using multiple breeding strategies.
ISSN:1380-3743
1572-9788
DOI:10.1007/s11032-021-01259-4