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Evaluation of cell transplant-mediated attenuation of diffuse injury in experimental autoimmune encephalomyelitis using onVDMP CEST MRI
The development and translation of cell therapies have been hindered by an inability to predict and evaluate their efficacy after transplantation. Using an experimental autoimmune encephalomyelitis (EAE) mouse model of multiple sclerosis (MS), we studied attenuation of the diffuse injury characteris...
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| Published in: | Experimental neurology 2020-07, Vol.329, p.113316-113316, Article 113316 |
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| container_title | Experimental neurology |
| container_volume | 329 |
| creator | Thomas, A.M. Li, S. Chu, C. Shats, I. Xu, J. Calabresi, P.A. van Zijl, P.C.M. Walczak, P. Bulte, J.W.M. |
| description | The development and translation of cell therapies have been hindered by an inability to predict and evaluate their efficacy after transplantation. Using an experimental autoimmune encephalomyelitis (EAE) mouse model of multiple sclerosis (MS), we studied attenuation of the diffuse injury characteristic of EAE and MS by transplanted glial-restricted precursor cells (GRPs). We assessed the potential of on-resonance variable delay multiple pulse (onVDMP) chemical exchange saturation transfer (CEST) MRI to visualize this attenuation. Allogeneic GRPs transplanted in the motor cortex or lateral ventricles attenuated paralysis in EAE mice and attenuated differences compared to naïve mice in onVDMP CEST signal 5 days after transplantation near the transplantation site. Histological analysis revealed that transplanted GRPs co-localized with attenuated astrogliosis. Hence, diffuse injury-sensitive onVDMP CEST MRI may complement conventional MRI to locate and monitor tissue regions responsive to GRP therapy.
•onVDMP CEST MRI signal changes in the brain as paralysis progresses•Stem cell transplantation in the motor cortex attenuates paralysis•onVDMP CEST MRI locates brain regions responsive to transplanted cell therapy |
| doi_str_mv | 10.1016/j.expneurol.2020.113316 |
| format | article |
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•onVDMP CEST MRI signal changes in the brain as paralysis progresses•Stem cell transplantation in the motor cortex attenuates paralysis•onVDMP CEST MRI locates brain regions responsive to transplanted cell therapy</description><identifier>ISSN: 0014-4886</identifier><identifier>EISSN: 1090-2430</identifier><identifier>DOI: 10.1016/j.expneurol.2020.113316</identifier><identifier>PMID: 32304749</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Animals ; Bioluminescence imaging ; Cell Transplantation - methods ; Chemical exchange saturation transfer ; Encephalomyelitis, Autoimmune, Experimental - diagnostic imaging ; Encephalomyelitis, Autoimmune, Experimental - metabolism ; Encephalomyelitis, Autoimmune, Experimental - therapy ; Glial-restricted precursor cells ; Luminescent Measurements - methods ; Magnetic resonance imaging ; Magnetic Resonance Imaging - methods ; Mice ; Mice, Transgenic ; Multiple sclerosis ; Neuroglia - metabolism ; Neuroglia - transplantation ; Transplantation</subject><ispartof>Experimental neurology, 2020-07, Vol.329, p.113316-113316, Article 113316</ispartof><rights>2020 Elsevier Inc.</rights><rights>Copyright © 2020 Elsevier Inc. All rights reserved.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c475t-480722a644a003f99da2554327d0a7eb380fef4be9c5245024456f9b538a98b43</citedby><cites>FETCH-LOGICAL-c475t-480722a644a003f99da2554327d0a7eb380fef4be9c5245024456f9b538a98b43</cites><orcidid>0000-0003-1202-1610</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>230,315,786,790,891,27957,27958</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/32304749$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Thomas, A.M.</creatorcontrib><creatorcontrib>Li, S.</creatorcontrib><creatorcontrib>Chu, C.</creatorcontrib><creatorcontrib>Shats, I.</creatorcontrib><creatorcontrib>Xu, J.</creatorcontrib><creatorcontrib>Calabresi, P.A.</creatorcontrib><creatorcontrib>van Zijl, P.C.M.</creatorcontrib><creatorcontrib>Walczak, P.</creatorcontrib><creatorcontrib>Bulte, J.W.M.</creatorcontrib><title>Evaluation of cell transplant-mediated attenuation of diffuse injury in experimental autoimmune encephalomyelitis using onVDMP CEST MRI</title><title>Experimental neurology</title><addtitle>Exp Neurol</addtitle><description>The development and translation of cell therapies have been hindered by an inability to predict and evaluate their efficacy after transplantation. Using an experimental autoimmune encephalomyelitis (EAE) mouse model of multiple sclerosis (MS), we studied attenuation of the diffuse injury characteristic of EAE and MS by transplanted glial-restricted precursor cells (GRPs). We assessed the potential of on-resonance variable delay multiple pulse (onVDMP) chemical exchange saturation transfer (CEST) MRI to visualize this attenuation. Allogeneic GRPs transplanted in the motor cortex or lateral ventricles attenuated paralysis in EAE mice and attenuated differences compared to naïve mice in onVDMP CEST signal 5 days after transplantation near the transplantation site. Histological analysis revealed that transplanted GRPs co-localized with attenuated astrogliosis. Hence, diffuse injury-sensitive onVDMP CEST MRI may complement conventional MRI to locate and monitor tissue regions responsive to GRP therapy.
•onVDMP CEST MRI signal changes in the brain as paralysis progresses•Stem cell transplantation in the motor cortex attenuates paralysis•onVDMP CEST MRI locates brain regions responsive to transplanted cell therapy</description><subject>Animals</subject><subject>Bioluminescence imaging</subject><subject>Cell Transplantation - methods</subject><subject>Chemical exchange saturation transfer</subject><subject>Encephalomyelitis, Autoimmune, Experimental - diagnostic imaging</subject><subject>Encephalomyelitis, Autoimmune, Experimental - metabolism</subject><subject>Encephalomyelitis, Autoimmune, Experimental - therapy</subject><subject>Glial-restricted precursor cells</subject><subject>Luminescent Measurements - methods</subject><subject>Magnetic resonance imaging</subject><subject>Magnetic Resonance Imaging - methods</subject><subject>Mice</subject><subject>Mice, Transgenic</subject><subject>Multiple sclerosis</subject><subject>Neuroglia - metabolism</subject><subject>Neuroglia - transplantation</subject><subject>Transplantation</subject><issn>0014-4886</issn><issn>1090-2430</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><recordid>eNqFUU1vEzEQtRCIhsJfAB-5bPDa3vX6glSlASq1AkHhanl3Z1tHXjv4IyK_gL-No5QAJ04jzbx58-Y9hF7VZFmTun2zWcKPrYMcvF1SQku3ZqxuH6FFTSSpKGfkMVoQUvOKd117hp7FuCGESE7FU3TGKCNccLlAP9c7bbNOxjvsJzyAtTgF7eLWapeqGUajE4xYpwTuD24005QjYOM2OexLwUUPBDODS9pinZM385wdYHADbO-19fMerEkm4hyNu8Pefbu8-YRX6y-3-Obz1XP0ZNI2wouHeo6-vlvfrj5U1x_fX60urquBiyaVZ4igVLeca0LYJOWoadNwRsVItICedWSCifcgh4byhlDOm3aSfcM6Lbues3P09si7zX15bih6g7ZqW6TrsFdeG_XvxJl7ded3SghBO0oLwesHguC_Z4hJzSYebNMOfI6KMllLwdpOFqg4QofgYwwwnc7URB1iVBt1ilEdYlTHGMvmy79VnvZ-51YAF0cAFK92BoKKgzlYPZoAQ1KjN_898gt9F7Zu</recordid><startdate>20200701</startdate><enddate>20200701</enddate><creator>Thomas, A.M.</creator><creator>Li, S.</creator><creator>Chu, C.</creator><creator>Shats, I.</creator><creator>Xu, J.</creator><creator>Calabresi, P.A.</creator><creator>van Zijl, P.C.M.</creator><creator>Walczak, P.</creator><creator>Bulte, J.W.M.</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>5PM</scope><orcidid>https://orcid.org/0000-0003-1202-1610</orcidid></search><sort><creationdate>20200701</creationdate><title>Evaluation of cell transplant-mediated attenuation of diffuse injury in experimental autoimmune encephalomyelitis using onVDMP CEST MRI</title><author>Thomas, A.M. ; Li, S. ; Chu, C. ; Shats, I. ; Xu, J. ; Calabresi, P.A. ; van Zijl, P.C.M. ; Walczak, P. ; Bulte, J.W.M.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c475t-480722a644a003f99da2554327d0a7eb380fef4be9c5245024456f9b538a98b43</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2020</creationdate><topic>Animals</topic><topic>Bioluminescence imaging</topic><topic>Cell Transplantation - methods</topic><topic>Chemical exchange saturation transfer</topic><topic>Encephalomyelitis, Autoimmune, Experimental - diagnostic imaging</topic><topic>Encephalomyelitis, Autoimmune, Experimental - metabolism</topic><topic>Encephalomyelitis, Autoimmune, Experimental - therapy</topic><topic>Glial-restricted precursor cells</topic><topic>Luminescent Measurements - methods</topic><topic>Magnetic resonance imaging</topic><topic>Magnetic Resonance Imaging - methods</topic><topic>Mice</topic><topic>Mice, Transgenic</topic><topic>Multiple sclerosis</topic><topic>Neuroglia - metabolism</topic><topic>Neuroglia - transplantation</topic><topic>Transplantation</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Thomas, A.M.</creatorcontrib><creatorcontrib>Li, S.</creatorcontrib><creatorcontrib>Chu, C.</creatorcontrib><creatorcontrib>Shats, I.</creatorcontrib><creatorcontrib>Xu, J.</creatorcontrib><creatorcontrib>Calabresi, P.A.</creatorcontrib><creatorcontrib>van Zijl, P.C.M.</creatorcontrib><creatorcontrib>Walczak, P.</creatorcontrib><creatorcontrib>Bulte, J.W.M.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Experimental neurology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Thomas, A.M.</au><au>Li, S.</au><au>Chu, C.</au><au>Shats, I.</au><au>Xu, J.</au><au>Calabresi, P.A.</au><au>van Zijl, P.C.M.</au><au>Walczak, P.</au><au>Bulte, J.W.M.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Evaluation of cell transplant-mediated attenuation of diffuse injury in experimental autoimmune encephalomyelitis using onVDMP CEST MRI</atitle><jtitle>Experimental neurology</jtitle><addtitle>Exp Neurol</addtitle><date>2020-07-01</date><risdate>2020</risdate><volume>329</volume><spage>113316</spage><epage>113316</epage><pages>113316-113316</pages><artnum>113316</artnum><issn>0014-4886</issn><eissn>1090-2430</eissn><notes>ObjectType-Article-1</notes><notes>SourceType-Scholarly Journals-1</notes><notes>ObjectType-Feature-2</notes><notes>content type line 23</notes><abstract>The development and translation of cell therapies have been hindered by an inability to predict and evaluate their efficacy after transplantation. Using an experimental autoimmune encephalomyelitis (EAE) mouse model of multiple sclerosis (MS), we studied attenuation of the diffuse injury characteristic of EAE and MS by transplanted glial-restricted precursor cells (GRPs). We assessed the potential of on-resonance variable delay multiple pulse (onVDMP) chemical exchange saturation transfer (CEST) MRI to visualize this attenuation. Allogeneic GRPs transplanted in the motor cortex or lateral ventricles attenuated paralysis in EAE mice and attenuated differences compared to naïve mice in onVDMP CEST signal 5 days after transplantation near the transplantation site. Histological analysis revealed that transplanted GRPs co-localized with attenuated astrogliosis. Hence, diffuse injury-sensitive onVDMP CEST MRI may complement conventional MRI to locate and monitor tissue regions responsive to GRP therapy.
•onVDMP CEST MRI signal changes in the brain as paralysis progresses•Stem cell transplantation in the motor cortex attenuates paralysis•onVDMP CEST MRI locates brain regions responsive to transplanted cell therapy</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>32304749</pmid><doi>10.1016/j.expneurol.2020.113316</doi><tpages>1</tpages><orcidid>https://orcid.org/0000-0003-1202-1610</orcidid><oa>free_for_read</oa></addata></record> |
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| subjects | Animals Bioluminescence imaging Cell Transplantation - methods Chemical exchange saturation transfer Encephalomyelitis, Autoimmune, Experimental - diagnostic imaging Encephalomyelitis, Autoimmune, Experimental - metabolism Encephalomyelitis, Autoimmune, Experimental - therapy Glial-restricted precursor cells Luminescent Measurements - methods Magnetic resonance imaging Magnetic Resonance Imaging - methods Mice Mice, Transgenic Multiple sclerosis Neuroglia - metabolism Neuroglia - transplantation Transplantation |
| title | Evaluation of cell transplant-mediated attenuation of diffuse injury in experimental autoimmune encephalomyelitis using onVDMP CEST MRI |
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