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WUSCHEL-related homeobox1 (WOX1) regulates vein patterning and leaf size in Cucumis sativus
Abstract In plants, WUSCHEL-related homeobox1 ( WOX1 ) homologs promote lamina mediolateral outgrowth. However, the downstream components linking WOX1 and lamina development remain unclear. In this study, we revealed the roles of WOX1 in palmate leaf expansion in cucumber ( Cucumis sativus ). A cucu...
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Published in: | Horticulture research 2020-11, Vol.7 (1), Article 182 |
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Main Authors: | , , , , , , , |
Format: | Article |
Language: | English |
Subjects: | |
Citations: | Items that this one cites Items that cite this one |
Online Access: | Get full text |
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Summary: | Abstract
In plants,
WUSCHEL-related homeobox1
(
WOX1
) homologs promote lamina mediolateral outgrowth. However, the downstream components linking WOX1 and lamina development remain unclear. In this study, we revealed the roles of WOX1 in palmate leaf expansion in cucumber (
Cucumis sativus
). A cucumber
mango fruit
(
mf
) mutant, resulting from truncation of a WOX1-type protein (CsWOX1), displayed abnormal lamina growth and defects in the development of secondary and smaller veins.
CsWOX1
was expressed in the middle mesophyll and leaf margins and rescued defects of the
Arabidopsis wox1 prs
double mutant. Transcriptomic analysis revealed that genes involved in auxin polar transport and auxin response were highly associated with leaf development. Analysis of the cucumber
mf rl
(
round leaf
) double mutant revealed that CsWOX1 functioned in vein development via PINOID (CsPID1)-controlled auxin transport. Overexpression of
CsWOX1
in cucumber (
CsWOX1
-OE) affected vein patterning and produced ‘butterfly-shaped’ leaves. CsWOX1 physically interacted with CsTCP4a, which may account for the abnormal lamina development in the
mf
mutant line and the smaller leaves in the
CsWOX1
-OE plants. Our findings demonstrated that CsWOX1 regulates cucumber leaf vein development by modulating auxin polar transport; moreover, CsWOX1 regulates leaf size by controlling
CIN-TCP
genes. |
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ISSN: | 2662-6810 2052-7276 |
DOI: | 10.1038/s41438-020-00404-y |