Design of a Bioreactor to Assess the Effect of Passive Joint Loading in a Live Chick Embryo In Ovo

There is increasing interest in understanding how mechanical cues (e.g., physical forces due to kicking and other movements) influence the embryological development of tissues and organs. For example, recent studies from our laboratory and others have used the chick embryo model to demonstrate that...

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Bibliographic Details
Published in:Tissue engineering. Part C, Methods Methods, 2019-11, Vol.25 (11), p.655-661
Main Authors: Stein, Matthew J., Buckley, Mark R., Manuele, Dylan, Gutierrez, Andrew, Loor, Jose Suarez, Nguyen, Phong K., Kuo, Catherine K.
Format: Article
Language:English
Subjects:
Ankle
Bioreactors
Embryos
Mechanical properties
Methods
Methods Article
Muscles
Tendons
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Summary:There is increasing interest in understanding how mechanical cues (e.g., physical forces due to kicking and other movements) influence the embryological development of tissues and organs. For example, recent studies from our laboratory and others have used the chick embryo model to demonstrate that the compositional and mechanical properties of developing tendons are strongly regulated by embryo movement frequency. However, current research tools for manipulating embryological movements and in ovo (or in utero ) mechanical forces are generally limited to chemical treatments that either paralyze or overstimulate muscles without allowing for precise control of physical cues. Thus, in this study, we introduce an instrument that enables application of passive, dynamic ankle flexion at prescribed amplitudes and frequencies in live, developing chick embryos. This device meets the design goals of allowing for precise (
ISSN:1937-3384
1937-3392
DOI:10.1089/ten.tec.2019.0114