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Activity-dependent presynaptic regulation of quantal size at the mammalian neuromuscular junction in vivo
Changes in synaptic activity alter quantal size, but the relative roles of presynaptic and postsynaptic cells in these changes are only beginning to be understood. We examined the mechanism underlying increased quantal size after block of synaptic activity at the mammalian neuromuscular junction in...
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| Published in: | The Journal of neuroscience 2005-01, Vol.25 (2), p.343-351 |
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| cites | cdi_FETCH-LOGICAL-c395t-c123862fb18dabc2bff18b1aebc28c8533100df89af715bfee2f0f8361bae2cf3 |
| container_end_page | 351 |
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| container_title | The Journal of neuroscience |
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| creator | Wang, Xueyong Li, Yingjie Engisch, Kathrin L Nakanishi, Stan T Dodson, Sara E Miller, Gary W Cope, Timothy C Pinter, Martin J Rich, Mark M |
| description | Changes in synaptic activity alter quantal size, but the relative roles of presynaptic and postsynaptic cells in these changes are only beginning to be understood. We examined the mechanism underlying increased quantal size after block of synaptic activity at the mammalian neuromuscular junction in vivo. We found that changes in neither acetylcholinesterase activity nor acetylcholine receptor density could account for the increase. By elimination, it appears likely that the site of increased quantal size after chronic block of activity is presynaptic and involves increased release of acetylcholine. We used mice with muscle hyperexcitability caused by mutation of the ClC-1 muscle chloride channel to examine the role of postsynaptic activity in controlling quantal size. Surprisingly, quantal size was increased in ClC mice before block of synaptic activity. We examined the mechanism underlying increased quantal size in ClC mice and found that it also appeared to be located presynaptically. When presynaptic activity was completely blocked in both control and ClC mice, quantal size was large in both groups despite the higher level of postsynaptic activity in ClC mice. This suggests that postsynaptic activity does not regulate quantal size at the neuromuscular junction. We propose that presynaptic activity modulates quantal size at the neuromuscular junction by modulating the amount of acetylcholine released from vesicles. |
| doi_str_mv | 10.1523/JNEUROSCI.3252-04.2005 |
| format | article |
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We examined the mechanism underlying increased quantal size after block of synaptic activity at the mammalian neuromuscular junction in vivo. We found that changes in neither acetylcholinesterase activity nor acetylcholine receptor density could account for the increase. By elimination, it appears likely that the site of increased quantal size after chronic block of activity is presynaptic and involves increased release of acetylcholine. We used mice with muscle hyperexcitability caused by mutation of the ClC-1 muscle chloride channel to examine the role of postsynaptic activity in controlling quantal size. Surprisingly, quantal size was increased in ClC mice before block of synaptic activity. We examined the mechanism underlying increased quantal size in ClC mice and found that it also appeared to be located presynaptically. When presynaptic activity was completely blocked in both control and ClC mice, quantal size was large in both groups despite the higher level of postsynaptic activity in ClC mice. This suggests that postsynaptic activity does not regulate quantal size at the neuromuscular junction. We propose that presynaptic activity modulates quantal size at the neuromuscular junction by modulating the amount of acetylcholine released from vesicles.</description><identifier>ISSN: 0270-6474</identifier><identifier>EISSN: 1529-2401</identifier><identifier>DOI: 10.1523/JNEUROSCI.3252-04.2005</identifier><identifier>PMID: 15647477</identifier><language>eng</language><publisher>United States: Society for Neuroscience</publisher><subject>Acetylcholinesterase - metabolism ; Animals ; Cellular/Molecular ; Chloride Channels - genetics ; Chloride Channels - physiology ; Electromyography ; Electrophysiology ; In Vitro Techniques ; Mice ; Mice, Knockout ; Motor Activity - physiology ; Motor Endplate - physiology ; Muscle Proteins - genetics ; Muscle Proteins - physiology ; Neuromuscular Junction - physiology ; Patch-Clamp Techniques ; Presynaptic Terminals - physiology ; Receptors, Cholinergic - metabolism ; Synaptic Vesicles - physiology</subject><ispartof>The Journal of neuroscience, 2005-01, Vol.25 (2), p.343-351</ispartof><rights>Copyright © 2005 Society for Neuroscience 0270-6474/05/25343-09.00/0 2005</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c395t-c123862fb18dabc2bff18b1aebc28c8533100df89af715bfee2f0f8361bae2cf3</citedby><cites>FETCH-LOGICAL-c395t-c123862fb18dabc2bff18b1aebc28c8533100df89af715bfee2f0f8361bae2cf3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC6725499/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC6725499/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,315,730,783,787,888,27936,27937,53804,53806</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/15647477$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Wang, Xueyong</creatorcontrib><creatorcontrib>Li, Yingjie</creatorcontrib><creatorcontrib>Engisch, Kathrin L</creatorcontrib><creatorcontrib>Nakanishi, Stan T</creatorcontrib><creatorcontrib>Dodson, Sara E</creatorcontrib><creatorcontrib>Miller, Gary W</creatorcontrib><creatorcontrib>Cope, Timothy C</creatorcontrib><creatorcontrib>Pinter, Martin J</creatorcontrib><creatorcontrib>Rich, Mark M</creatorcontrib><title>Activity-dependent presynaptic regulation of quantal size at the mammalian neuromuscular junction in vivo</title><title>The Journal of neuroscience</title><addtitle>J Neurosci</addtitle><description>Changes in synaptic activity alter quantal size, but the relative roles of presynaptic and postsynaptic cells in these changes are only beginning to be understood. We examined the mechanism underlying increased quantal size after block of synaptic activity at the mammalian neuromuscular junction in vivo. We found that changes in neither acetylcholinesterase activity nor acetylcholine receptor density could account for the increase. By elimination, it appears likely that the site of increased quantal size after chronic block of activity is presynaptic and involves increased release of acetylcholine. We used mice with muscle hyperexcitability caused by mutation of the ClC-1 muscle chloride channel to examine the role of postsynaptic activity in controlling quantal size. Surprisingly, quantal size was increased in ClC mice before block of synaptic activity. We examined the mechanism underlying increased quantal size in ClC mice and found that it also appeared to be located presynaptically. When presynaptic activity was completely blocked in both control and ClC mice, quantal size was large in both groups despite the higher level of postsynaptic activity in ClC mice. This suggests that postsynaptic activity does not regulate quantal size at the neuromuscular junction. We propose that presynaptic activity modulates quantal size at the neuromuscular junction by modulating the amount of acetylcholine released from vesicles.</description><subject>Acetylcholinesterase - metabolism</subject><subject>Animals</subject><subject>Cellular/Molecular</subject><subject>Chloride Channels - genetics</subject><subject>Chloride Channels - physiology</subject><subject>Electromyography</subject><subject>Electrophysiology</subject><subject>In Vitro Techniques</subject><subject>Mice</subject><subject>Mice, Knockout</subject><subject>Motor Activity - physiology</subject><subject>Motor Endplate - physiology</subject><subject>Muscle Proteins - genetics</subject><subject>Muscle Proteins - physiology</subject><subject>Neuromuscular Junction - physiology</subject><subject>Patch-Clamp Techniques</subject><subject>Presynaptic Terminals - physiology</subject><subject>Receptors, Cholinergic - metabolism</subject><subject>Synaptic Vesicles - physiology</subject><issn>0270-6474</issn><issn>1529-2401</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2005</creationdate><recordtype>article</recordtype><recordid>eNqFkVtr3DAQhUVJaDZp_0LQU9681cWy7JdCWHIlNJDLs5DlUaJgS44kL2x-fbzNkrZPfZph5pzDDB9Cx5QsqWD8x_Wvs8e72_vV1ZIzwQpSLhkh4gtazNumYCWhe2hBmCRFVcryAB2m9EIIkYTKr-iAiu1UygVypya7tcubooMRfAc-4zFC2ng9ZmdwhKep19kFj4PFr5P2Wfc4uTfAOuP8DHjQw6B7pz32MMUwTMnMjohfJm9--5zHa7cO39C-1X2C77t6hB7Pzx5Wl8XN7cXV6vSmMLwRuTCU8bpitqV1p1vDWmtp3VINc1-bWnBOCels3WgrqWgtALPE1ryirQZmLD9CPz9yx6kdoDPzR1H3aoxu0HGjgnbq3413z-oprFUlmSibZg442QXE8DpBympwyUDfaw9hSrOOV5I21X-FVApWNhWdhdWH0MSQUgT7eQ0laotTfeJUW5yKlGqLczYe__3LH9uOH38Hllqhpw</recordid><startdate>20050112</startdate><enddate>20050112</enddate><creator>Wang, Xueyong</creator><creator>Li, Yingjie</creator><creator>Engisch, Kathrin L</creator><creator>Nakanishi, Stan T</creator><creator>Dodson, Sara E</creator><creator>Miller, Gary W</creator><creator>Cope, Timothy C</creator><creator>Pinter, Martin J</creator><creator>Rich, Mark M</creator><general>Society for Neuroscience</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TK</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20050112</creationdate><title>Activity-dependent presynaptic regulation of quantal size at the mammalian neuromuscular junction in vivo</title><author>Wang, Xueyong ; 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| ispartof | The Journal of neuroscience, 2005-01, Vol.25 (2), p.343-351 |
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| source | PubMed Central |
| subjects | Acetylcholinesterase - metabolism Animals Cellular/Molecular Chloride Channels - genetics Chloride Channels - physiology Electromyography Electrophysiology In Vitro Techniques Mice Mice, Knockout Motor Activity - physiology Motor Endplate - physiology Muscle Proteins - genetics Muscle Proteins - physiology Neuromuscular Junction - physiology Patch-Clamp Techniques Presynaptic Terminals - physiology Receptors, Cholinergic - metabolism Synaptic Vesicles - physiology |
| title | Activity-dependent presynaptic regulation of quantal size at the mammalian neuromuscular junction in vivo |
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