In-depth analysis of swim bladder-associated microbiota in rainbow trout (Oncorhynchus mykiss)

Our knowledge regarding microbiota associated with the swim bladder of physostomous, fish with the swim bladder connected to the esophagus via the pneumatic duct, remains largely unknown. The goal of this study was to conduct the first in-depth characterization of the swim bladder-associated microbi...

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Bibliographic Details
Published in:Scientific reports 2019-06, Vol.9 (1), p.8974-12, Article 8974
Main Authors: Villasante, Alejandro, Ramírez, Carolina, Rodríguez, Héctor, Catalán, Natalia, Díaz, Osmán, Rojas, Rodrigo, Opazo, Rafael, Romero, Jaime
Format: Article
Language:English
Subjects:
Air Sacs - microbiology
Amino acids
Animals
Carbohydrates
Cell growth
Computational Biology - methods
Esophagus
Fatty acids
Gastrointestinal Microbiome
Growth disorders
Intestine
Lipoic acid
Metabolic pathways
Metabolism
Metagenome
Metagenomics - methods
Microbiota
Next-generation sequencing
Nucleotides
Oncorhynchus mykiss
Oxidative phosphorylation
Phosphorylation
rRNA
Secretion
Swim bladder
Symbiosis
Trout
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Summary:Our knowledge regarding microbiota associated with the swim bladder of physostomous, fish with the swim bladder connected to the esophagus via the pneumatic duct, remains largely unknown. The goal of this study was to conduct the first in-depth characterization of the swim bladder-associated microbiota using high-throughput sequencing of the V4 region of the 16 S rRNA gene in rainbow trout (Oncorhynchus mykiss). We observed major differences in bacterial communities composition between swim bladder-associated microbiota and distal intestine digesta microbiota in fish. Whilst bacteria genera, such as Cohnella, Lactococcus and Mycoplasma were more abundant in swim bladder-associated microbiota, Citrobacter, Rhodobacter and Clavibacter were more abundant in distal intestine digesta microbiota. The presumptive metabolic function analysis (PICRUSt) revealed several metabolic pathways to be more abundant in the swim bladder-associated microbiota, including metabolism of carbohydrates, nucleotides and lipoic acid as well as oxidative phosphorylation, cell growth, translation, replication and repair. Distal intestine digesta microbiota showed greater abundance of nitrogen metabolism, amino acid metabolism, biosynthesis of unsaturated fatty acids and bacterial secretion system. We demonstrated swim bladder harbors a unique microbiota, which composition and metabolic function differ from microbiota associated with the gut in fish.
ISSN:2045-2322
2045-2322
DOI:10.1038/s41598-019-45451-1