Circulating Microbiota-Based Metagenomic Signature for Detection of Hepatocellular Carcinoma

Circulating microbial dysbiosis is associated with chronic liver disease including nonalcoholic steatohepatitis and alcoholic liver disease. In this study, we evaluated whether disease-specific alterations of circulating microbiome are present in patients with cirrhosis and hepatocellular carcinoma...

Full description

Saved in:
Bibliographic Details
Published in:Scientific reports 2019-05, Vol.9 (1), p.7536-8, Article 7536
Main Authors: Cho, Eun Ju, Leem, Sangseob, Kim, Sun Ah, Yang, Jinho, Lee, Yun Bin, Kim, Soon Sun, Cheong, Jae Youn, Cho, Sung Won, Kim, Ji Won, Kim, Sung-Min, Yoon, Jung-Hwan, Park, Taesung
Format: Article
Language:English
Subjects:
Biomarkers
Biomarkers, Tumor - genetics
Carcinoma, Hepatocellular - blood
Carcinoma, Hepatocellular - diagnosis
Carcinoma, Hepatocellular - microbiology
Cirrhosis
Cross-Sectional Studies
DNA, Bacterial - blood
DNA, Ribosomal - blood
DNA, Ribosomal - genetics
Dysbacteriosis
Dysbiosis - blood
Dysbiosis - microbiology
Female
Hepatocellular carcinoma
Humans
Liver cancer
Liver cirrhosis
Liver Cirrhosis - blood
Liver Cirrhosis - microbiology
Liver diseases
Liver Neoplasms - blood
Liver Neoplasms - diagnosis
Liver Neoplasms - microbiology
Male
Metagenome - genetics
Microbiomes
Microbiota
Microbiota - genetics
Middle Aged
Republic of Korea
RNA, Ribosomal, 16S - genetics
rRNA 16S
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Circulating microbial dysbiosis is associated with chronic liver disease including nonalcoholic steatohepatitis and alcoholic liver disease. In this study, we evaluated whether disease-specific alterations of circulating microbiome are present in patients with cirrhosis and hepatocellular carcinoma (HCC), and their potential as diagnostic biomarkers for HCC. We performed cross-sectional metagenomic analyses of serum samples from 79 patients with HCC, 83 with cirrhosis, and 201 matching healthy controls, and validated the results in the same number of subjects. Serum bacterial DNA was analyzed using high-throughput pyrosequencing after amplification of the V3-V4 hypervariable regions of 16S rDNA. Blood microbial diversity was significantly reduced in HCC, compared with cirrhosis and control. There were significant differences in the relative abundances of several bacterial taxa that correlate with the presence of HCC, thus defining a specific blood microbiome-derived metagenomic signature of HCC. We identified 5 microbial gene markers-based model which distinguished HCC from controls with an area under the receiver-operating curve (AUC) of 0.879 and a balanced accuracy of 81.6%. In the validation, this model accurately distinguished HCC with an AUC of 0.875 and an accuracy of 79.8%. In conclusion, circulating microbiome-based signatures may be potential biomarkers for the detection HCC.
ISSN:2045-2322
2045-2322
DOI:10.1038/s41598-019-44012-w