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Transcutaneous oxygen measurement in humans using a paramagnetic skin adhesive film
Purpose Transcutaneous oxygen tension (TcpO2) provides information about blood perfusion in the tissue immediately below the skin. These data are valuable in assessing wound healing problems, diagnosing peripheral vascular/arterial insufficiency, and predicting disease progression or the response to...
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| Published in: | Magnetic resonance in medicine 2019-02, Vol.81 (2), p.781-794 |
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| container_end_page | 794 |
| container_issue | 2 |
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| container_title | Magnetic resonance in medicine |
| container_volume | 81 |
| creator | Kmiec, Maciej M. Hou, Huagang Lakshmi Kuppusamy, M. Drews, Thomas M. Prabhat, Anjali M. Petryakov, Sergey V. Demidenko, Eugene Schaner, Philip E. Buckey, Jay C. Blank, Aharon Kuppusamy, Periannan |
| description | Purpose
Transcutaneous oxygen tension (TcpO2) provides information about blood perfusion in the tissue immediately below the skin. These data are valuable in assessing wound healing problems, diagnosing peripheral vascular/arterial insufficiency, and predicting disease progression or the response to therapy. Currently, TcpO2 is primarily measured using electrochemical skin sensors, which consume oxygen and are prone to calibration errors. The goal of the present study was to develop a reliable method for TcpO2 measurement in human subjects.
Methods
We have developed a novel TcpO2 oximetry method based on electron paramagnetic resonance (EPR) principles with an oxygen‐sensing skin adhesive film, named the superficial perfusion oxygen tension (SPOT) chip. The SPOT chip is a 3‐mm diameter, 60‐μm thick circular film composed of a stable paramagnetic oxygen sensor. The chip is covered with an oxygen‐barrier material on one side and secured on the skin by a medical adhesive transfer tape to ensure that only the oxygen that diffuses through the skin surface is measured. The method quantifies TcpO2 through the linewidth of the EPR spectrum.
Results
Repeated measurements using a cohort of 10 healthy human subjects showed that the TcpO2 measurements were robust, reliable, and reproducible. The TcpO2 values ranged from 7.8 ± 0.8 to 22.0 ± 1.0 mmHg in the volar forearm skin (N = 29) and 8.1 ± 0.3 to 23.4 ± 1.3 mmHg in the foot (N = 86).
Conclusions
The results demonstrated that the SPOT chip can measure TcpO2 reliably and repeatedly under ambient conditions. The SPOT chip method could potentially be used to monitor TcpO2 in the clinic. |
| doi_str_mv | 10.1002/mrm.27445 |
| format | article |
| fullrecord | <record><control><sourceid>proquest_pubme</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_6289671</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2153940893</sourcerecordid><originalsourceid>FETCH-LOGICAL-c5095-a3e87956b3c26fe22f3247024b19e41319ffea39ae660000ca11e6ef3223716a3</originalsourceid><addsrcrecordid>eNp10U9PFDEYBvCGaGRBD3wB08QLHAb6v9uLCSGCJhATxXPz7vDObnHaWdsdcL89lUGiJp566C9PnvYh5ICzY86YOIk5HgurlN4hM66FaIR26gWZMatYI7lTu2SvlFvGmHNWvSK7kglrhdUz8vU6QyrtuIGEw1jo8HO7xEQjQhkzRkwbGhJdjbEqOpaQlhToGjJEWCbchJaW7xXAzQpLuEPahT6-Ji876Au-eTr3ybfzD9dnH5vLzxefzk4vm1YzpxuQOLdOm4VshelQiE4KZZlQC-5Q8dq76xCkAzSmVmctcI4GqxLScgNyn7yfctfjIuJNW8tm6P06hwh56wcI_u-bFFZ-Odx5I-bOWF4DDp8C8vBjxLLxMZQW-376DC8411YLzU2l7_6ht8OYU31eVVo6xeZOVnU0qTYPpWTsnstw5n9N5etU_nGqat_-2f5Z_t6mgpMJ3Icet_9P8ldfrqbIBx2WnmM</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2153940893</pqid></control><display><type>article</type><title>Transcutaneous oxygen measurement in humans using a paramagnetic skin adhesive film</title><source>Wiley-Blackwell Journals</source><creator>Kmiec, Maciej M. ; Hou, Huagang ; Lakshmi Kuppusamy, M. ; Drews, Thomas M. ; Prabhat, Anjali M. ; Petryakov, Sergey V. ; Demidenko, Eugene ; Schaner, Philip E. ; Buckey, Jay C. ; Blank, Aharon ; Kuppusamy, Periannan</creator><creatorcontrib>Kmiec, Maciej M. ; Hou, Huagang ; Lakshmi Kuppusamy, M. ; Drews, Thomas M. ; Prabhat, Anjali M. ; Petryakov, Sergey V. ; Demidenko, Eugene ; Schaner, Philip E. ; Buckey, Jay C. ; Blank, Aharon ; Kuppusamy, Periannan</creatorcontrib><description>Purpose
Transcutaneous oxygen tension (TcpO2) provides information about blood perfusion in the tissue immediately below the skin. These data are valuable in assessing wound healing problems, diagnosing peripheral vascular/arterial insufficiency, and predicting disease progression or the response to therapy. Currently, TcpO2 is primarily measured using electrochemical skin sensors, which consume oxygen and are prone to calibration errors. The goal of the present study was to develop a reliable method for TcpO2 measurement in human subjects.
Methods
We have developed a novel TcpO2 oximetry method based on electron paramagnetic resonance (EPR) principles with an oxygen‐sensing skin adhesive film, named the superficial perfusion oxygen tension (SPOT) chip. The SPOT chip is a 3‐mm diameter, 60‐μm thick circular film composed of a stable paramagnetic oxygen sensor. The chip is covered with an oxygen‐barrier material on one side and secured on the skin by a medical adhesive transfer tape to ensure that only the oxygen that diffuses through the skin surface is measured. The method quantifies TcpO2 through the linewidth of the EPR spectrum.
Results
Repeated measurements using a cohort of 10 healthy human subjects showed that the TcpO2 measurements were robust, reliable, and reproducible. The TcpO2 values ranged from 7.8 ± 0.8 to 22.0 ± 1.0 mmHg in the volar forearm skin (N = 29) and 8.1 ± 0.3 to 23.4 ± 1.3 mmHg in the foot (N = 86).
Conclusions
The results demonstrated that the SPOT chip can measure TcpO2 reliably and repeatedly under ambient conditions. The SPOT chip method could potentially be used to monitor TcpO2 in the clinic.</description><identifier>ISSN: 0740-3194</identifier><identifier>EISSN: 1522-2594</identifier><identifier>DOI: 10.1002/mrm.27445</identifier><identifier>PMID: 30277275</identifier><language>eng</language><publisher>United States: Wiley Subscription Services, Inc</publisher><subject>Adhesion tests ; Adhesives ; Adolescent ; Adult ; Arterial Occlusive Diseases - physiopathology ; Calibration ; Chemical sensors ; Cohort Studies ; Electrochemistry ; Electron paramagnetic resonance ; Electron Spin Resonance Spectroscopy ; EPR ; Female ; Foot ; Forearm ; Healthy Volunteers ; Human subjects ; Humans ; Male ; Middle Aged ; Oximetry ; Oxygen ; Oxygen - analysis ; Oxygen - blood ; Oxygen probes ; Oxygen tension ; Perfusion ; Peripheral Vascular Diseases - physiopathology ; Reproducibility of Results ; Skin ; Skin - blood supply ; Skin Physiological Phenomena ; SPOT chip ; TcOM ; Temperature ; Tension ; Transcutaneous ; Wound Healing ; Young Adult</subject><ispartof>Magnetic resonance in medicine, 2019-02, Vol.81 (2), p.781-794</ispartof><rights>2018 International Society for Magnetic Resonance in Medicine</rights><rights>2018 International Society for Magnetic Resonance in Medicine.</rights><rights>2019 International Society for Magnetic Resonance in Medicine</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c5095-a3e87956b3c26fe22f3247024b19e41319ffea39ae660000ca11e6ef3223716a3</citedby><cites>FETCH-LOGICAL-c5095-a3e87956b3c26fe22f3247024b19e41319ffea39ae660000ca11e6ef3223716a3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Fmrm.27445$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Fmrm.27445$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>230,315,786,790,891,27957,27958,50923,51032</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/30277275$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Kmiec, Maciej M.</creatorcontrib><creatorcontrib>Hou, Huagang</creatorcontrib><creatorcontrib>Lakshmi Kuppusamy, M.</creatorcontrib><creatorcontrib>Drews, Thomas M.</creatorcontrib><creatorcontrib>Prabhat, Anjali M.</creatorcontrib><creatorcontrib>Petryakov, Sergey V.</creatorcontrib><creatorcontrib>Demidenko, Eugene</creatorcontrib><creatorcontrib>Schaner, Philip E.</creatorcontrib><creatorcontrib>Buckey, Jay C.</creatorcontrib><creatorcontrib>Blank, Aharon</creatorcontrib><creatorcontrib>Kuppusamy, Periannan</creatorcontrib><title>Transcutaneous oxygen measurement in humans using a paramagnetic skin adhesive film</title><title>Magnetic resonance in medicine</title><addtitle>Magn Reson Med</addtitle><description>Purpose
Transcutaneous oxygen tension (TcpO2) provides information about blood perfusion in the tissue immediately below the skin. These data are valuable in assessing wound healing problems, diagnosing peripheral vascular/arterial insufficiency, and predicting disease progression or the response to therapy. Currently, TcpO2 is primarily measured using electrochemical skin sensors, which consume oxygen and are prone to calibration errors. The goal of the present study was to develop a reliable method for TcpO2 measurement in human subjects.
Methods
We have developed a novel TcpO2 oximetry method based on electron paramagnetic resonance (EPR) principles with an oxygen‐sensing skin adhesive film, named the superficial perfusion oxygen tension (SPOT) chip. The SPOT chip is a 3‐mm diameter, 60‐μm thick circular film composed of a stable paramagnetic oxygen sensor. The chip is covered with an oxygen‐barrier material on one side and secured on the skin by a medical adhesive transfer tape to ensure that only the oxygen that diffuses through the skin surface is measured. The method quantifies TcpO2 through the linewidth of the EPR spectrum.
Results
Repeated measurements using a cohort of 10 healthy human subjects showed that the TcpO2 measurements were robust, reliable, and reproducible. The TcpO2 values ranged from 7.8 ± 0.8 to 22.0 ± 1.0 mmHg in the volar forearm skin (N = 29) and 8.1 ± 0.3 to 23.4 ± 1.3 mmHg in the foot (N = 86).
Conclusions
The results demonstrated that the SPOT chip can measure TcpO2 reliably and repeatedly under ambient conditions. The SPOT chip method could potentially be used to monitor TcpO2 in the clinic.</description><subject>Adhesion tests</subject><subject>Adhesives</subject><subject>Adolescent</subject><subject>Adult</subject><subject>Arterial Occlusive Diseases - physiopathology</subject><subject>Calibration</subject><subject>Chemical sensors</subject><subject>Cohort Studies</subject><subject>Electrochemistry</subject><subject>Electron paramagnetic resonance</subject><subject>Electron Spin Resonance Spectroscopy</subject><subject>EPR</subject><subject>Female</subject><subject>Foot</subject><subject>Forearm</subject><subject>Healthy Volunteers</subject><subject>Human subjects</subject><subject>Humans</subject><subject>Male</subject><subject>Middle Aged</subject><subject>Oximetry</subject><subject>Oxygen</subject><subject>Oxygen - analysis</subject><subject>Oxygen - blood</subject><subject>Oxygen probes</subject><subject>Oxygen tension</subject><subject>Perfusion</subject><subject>Peripheral Vascular Diseases - physiopathology</subject><subject>Reproducibility of Results</subject><subject>Skin</subject><subject>Skin - blood supply</subject><subject>Skin Physiological Phenomena</subject><subject>SPOT chip</subject><subject>TcOM</subject><subject>Temperature</subject><subject>Tension</subject><subject>Transcutaneous</subject><subject>Wound Healing</subject><subject>Young Adult</subject><issn>0740-3194</issn><issn>1522-2594</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2019</creationdate><recordtype>article</recordtype><recordid>eNp10U9PFDEYBvCGaGRBD3wB08QLHAb6v9uLCSGCJhATxXPz7vDObnHaWdsdcL89lUGiJp566C9PnvYh5ICzY86YOIk5HgurlN4hM66FaIR26gWZMatYI7lTu2SvlFvGmHNWvSK7kglrhdUz8vU6QyrtuIGEw1jo8HO7xEQjQhkzRkwbGhJdjbEqOpaQlhToGjJEWCbchJaW7xXAzQpLuEPahT6-Ji876Au-eTr3ybfzD9dnH5vLzxefzk4vm1YzpxuQOLdOm4VshelQiE4KZZlQC-5Q8dq76xCkAzSmVmctcI4GqxLScgNyn7yfctfjIuJNW8tm6P06hwh56wcI_u-bFFZ-Odx5I-bOWF4DDp8C8vBjxLLxMZQW-376DC8411YLzU2l7_6ht8OYU31eVVo6xeZOVnU0qTYPpWTsnstw5n9N5etU_nGqat_-2f5Z_t6mgpMJ3Icet_9P8ldfrqbIBx2WnmM</recordid><startdate>201902</startdate><enddate>201902</enddate><creator>Kmiec, Maciej M.</creator><creator>Hou, Huagang</creator><creator>Lakshmi Kuppusamy, M.</creator><creator>Drews, Thomas M.</creator><creator>Prabhat, Anjali M.</creator><creator>Petryakov, Sergey V.</creator><creator>Demidenko, Eugene</creator><creator>Schaner, Philip E.</creator><creator>Buckey, Jay C.</creator><creator>Blank, Aharon</creator><creator>Kuppusamy, Periannan</creator><general>Wiley Subscription Services, Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>8FD</scope><scope>FR3</scope><scope>K9.</scope><scope>M7Z</scope><scope>P64</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>201902</creationdate><title>Transcutaneous oxygen measurement in humans using a paramagnetic skin adhesive film</title><author>Kmiec, Maciej M. ; Hou, Huagang ; Lakshmi Kuppusamy, M. ; Drews, Thomas M. ; Prabhat, Anjali M. ; Petryakov, Sergey V. ; Demidenko, Eugene ; Schaner, Philip E. ; Buckey, Jay C. ; Blank, Aharon ; Kuppusamy, Periannan</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c5095-a3e87956b3c26fe22f3247024b19e41319ffea39ae660000ca11e6ef3223716a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2019</creationdate><topic>Adhesion tests</topic><topic>Adhesives</topic><topic>Adolescent</topic><topic>Adult</topic><topic>Arterial Occlusive Diseases - physiopathology</topic><topic>Calibration</topic><topic>Chemical sensors</topic><topic>Cohort Studies</topic><topic>Electrochemistry</topic><topic>Electron paramagnetic resonance</topic><topic>Electron Spin Resonance Spectroscopy</topic><topic>EPR</topic><topic>Female</topic><topic>Foot</topic><topic>Forearm</topic><topic>Healthy Volunteers</topic><topic>Human subjects</topic><topic>Humans</topic><topic>Male</topic><topic>Middle Aged</topic><topic>Oximetry</topic><topic>Oxygen</topic><topic>Oxygen - analysis</topic><topic>Oxygen - blood</topic><topic>Oxygen probes</topic><topic>Oxygen tension</topic><topic>Perfusion</topic><topic>Peripheral Vascular Diseases - physiopathology</topic><topic>Reproducibility of Results</topic><topic>Skin</topic><topic>Skin - blood supply</topic><topic>Skin Physiological Phenomena</topic><topic>SPOT chip</topic><topic>TcOM</topic><topic>Temperature</topic><topic>Tension</topic><topic>Transcutaneous</topic><topic>Wound Healing</topic><topic>Young Adult</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kmiec, Maciej M.</creatorcontrib><creatorcontrib>Hou, Huagang</creatorcontrib><creatorcontrib>Lakshmi Kuppusamy, M.</creatorcontrib><creatorcontrib>Drews, Thomas M.</creatorcontrib><creatorcontrib>Prabhat, Anjali M.</creatorcontrib><creatorcontrib>Petryakov, Sergey V.</creatorcontrib><creatorcontrib>Demidenko, Eugene</creatorcontrib><creatorcontrib>Schaner, Philip E.</creatorcontrib><creatorcontrib>Buckey, Jay C.</creatorcontrib><creatorcontrib>Blank, Aharon</creatorcontrib><creatorcontrib>Kuppusamy, Periannan</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Biochemistry Abstracts 1</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Magnetic resonance in medicine</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kmiec, Maciej M.</au><au>Hou, Huagang</au><au>Lakshmi Kuppusamy, M.</au><au>Drews, Thomas M.</au><au>Prabhat, Anjali M.</au><au>Petryakov, Sergey V.</au><au>Demidenko, Eugene</au><au>Schaner, Philip E.</au><au>Buckey, Jay C.</au><au>Blank, Aharon</au><au>Kuppusamy, Periannan</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Transcutaneous oxygen measurement in humans using a paramagnetic skin adhesive film</atitle><jtitle>Magnetic resonance in medicine</jtitle><addtitle>Magn Reson Med</addtitle><date>2019-02</date><risdate>2019</risdate><volume>81</volume><issue>2</issue><spage>781</spage><epage>794</epage><pages>781-794</pages><issn>0740-3194</issn><eissn>1522-2594</eissn><notes>ObjectType-Article-1</notes><notes>SourceType-Scholarly Journals-1</notes><notes>ObjectType-Feature-2</notes><notes>content type line 23</notes><abstract>Purpose
Transcutaneous oxygen tension (TcpO2) provides information about blood perfusion in the tissue immediately below the skin. These data are valuable in assessing wound healing problems, diagnosing peripheral vascular/arterial insufficiency, and predicting disease progression or the response to therapy. Currently, TcpO2 is primarily measured using electrochemical skin sensors, which consume oxygen and are prone to calibration errors. The goal of the present study was to develop a reliable method for TcpO2 measurement in human subjects.
Methods
We have developed a novel TcpO2 oximetry method based on electron paramagnetic resonance (EPR) principles with an oxygen‐sensing skin adhesive film, named the superficial perfusion oxygen tension (SPOT) chip. The SPOT chip is a 3‐mm diameter, 60‐μm thick circular film composed of a stable paramagnetic oxygen sensor. The chip is covered with an oxygen‐barrier material on one side and secured on the skin by a medical adhesive transfer tape to ensure that only the oxygen that diffuses through the skin surface is measured. The method quantifies TcpO2 through the linewidth of the EPR spectrum.
Results
Repeated measurements using a cohort of 10 healthy human subjects showed that the TcpO2 measurements were robust, reliable, and reproducible. The TcpO2 values ranged from 7.8 ± 0.8 to 22.0 ± 1.0 mmHg in the volar forearm skin (N = 29) and 8.1 ± 0.3 to 23.4 ± 1.3 mmHg in the foot (N = 86).
Conclusions
The results demonstrated that the SPOT chip can measure TcpO2 reliably and repeatedly under ambient conditions. The SPOT chip method could potentially be used to monitor TcpO2 in the clinic.</abstract><cop>United States</cop><pub>Wiley Subscription Services, Inc</pub><pmid>30277275</pmid><doi>10.1002/mrm.27445</doi><tpages>0</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Magnetic resonance in medicine, 2019-02, Vol.81 (2), p.781-794 |
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| source | Wiley-Blackwell Journals |
| subjects | Adhesion tests Adhesives Adolescent Adult Arterial Occlusive Diseases - physiopathology Calibration Chemical sensors Cohort Studies Electrochemistry Electron paramagnetic resonance Electron Spin Resonance Spectroscopy EPR Female Foot Forearm Healthy Volunteers Human subjects Humans Male Middle Aged Oximetry Oxygen Oxygen - analysis Oxygen - blood Oxygen probes Oxygen tension Perfusion Peripheral Vascular Diseases - physiopathology Reproducibility of Results Skin Skin - blood supply Skin Physiological Phenomena SPOT chip TcOM Temperature Tension Transcutaneous Wound Healing Young Adult |
| title | Transcutaneous oxygen measurement in humans using a paramagnetic skin adhesive film |
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