Loading…
Backbone-free duplex-stacked monomer nucleic acids exhibiting Watson–Crick selectivity
We demonstrate that nucleic acid (NA) mononucleotide triphosphates (dNTPs and rNTPs), at sufficiently high concentration and low temperature in aqueous solution, can exhibit a phase transition in which chromonic columnar liquid crystal ordering spontaneously appears. Remarkably, this polymer-free st...
Saved in:
| Published in: | Proceedings of the National Academy of Sciences - PNAS 2018-08, Vol.115 (33), p.E7658-E7664 |
|---|---|
| Main Authors: | , , , , , , , |
| Format: | Article |
| Language: | English |
| Subjects: | |
| Citations: | Items that this one cites Items that cite this one |
| Online Access: | Get full text |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| cited_by | cdi_FETCH-LOGICAL-c536t-175f136c1420a56e83c8d1d5190639a7f42450f65a4efa37d61aef2b0260c3733 |
|---|---|
| cites | cdi_FETCH-LOGICAL-c536t-175f136c1420a56e83c8d1d5190639a7f42450f65a4efa37d61aef2b0260c3733 |
| container_end_page | E7664 |
| container_issue | 33 |
| container_start_page | E7658 |
| container_title | Proceedings of the National Academy of Sciences - PNAS |
| container_volume | 115 |
| creator | Smith, Gregory P. Fraccia, Tommaso P. Todisco, Marco Zanchetta, Giuliano Zhu, Chenhui Hayden, Emily Bellini, Tommaso Clark, Noel A. |
| description | We demonstrate that nucleic acid (NA) mononucleotide triphosphates (dNTPs and rNTPs), at sufficiently high concentration and low temperature in aqueous solution, can exhibit a phase transition in which chromonic columnar liquid crystal ordering spontaneously appears. Remarkably, this polymer-free state exhibits, in a self-assembly of NA monomers, the key structural elements of biological nucleic acids, including: long-ranged duplex stacking of base pairs, complementarity-dependent partitioning of molecules, and Watson–Crick selectivity, such that, among all solutions of adenosine, cytosine, guanine, and thymine NTPs and their binary mixtures, duplex columnar ordering is most stable in the A-T and C-G combinations. |
| doi_str_mv | 10.1073/pnas.1721369115 |
| format | article |
| fullrecord | <record><control><sourceid>jstor_pubme</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_6099888</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><jstor_id>26530263</jstor_id><sourcerecordid>26530263</sourcerecordid><originalsourceid>FETCH-LOGICAL-c536t-175f136c1420a56e83c8d1d5190639a7f42450f65a4efa37d61aef2b0260c3733</originalsourceid><addsrcrecordid>eNpdkb9uFDEQxi0EIkegpgKtoKHZxGOv7XWDBCf-SZFoQNBZPu9szpc9-2J7o6TjHXhDngRHFxKgGsnz8zffzEfIU6BHQBU_3gWbj0Ax4FIDiHtkAVRDKztN75MFpUy1fce6A_Io5w2lVIuePiQHTGupQOoF-f7WurNVDNiOCbEZ5t2El20u9RWHZhtD3GJqwuwm9K6xzg-5wcu1X_niw2nzzZYcw68fP5fJu7Mm44Su-Atfrh6TB6OdMj65qYfk6_t3X5Yf25PPHz4t35y0TnBZWlBirOYddIxaIbHnrh9gEKCp5NqqsboXdJTCdjhargYJFke2okxSxxXnh-T1Xnc3r7Y4OAwl2cnskt_adGWi9ebfTvBrcxovjKRa931fBV7sBWIu3mTnC7q1iyHUTQxIJiiwCr26mZLi-Yy5mK3PDqfJBoxzNqy6VaAV0Iq-_A_dxDmFeoNKaaUV61lXqeM95VLMOeF46xiouY7WXEdr7qKtP57_vegt_yfLCjzbA5tcYrrrS8HrtTj_DXJTqn8</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2097972824</pqid></control><display><type>article</type><title>Backbone-free duplex-stacked monomer nucleic acids exhibiting Watson–Crick selectivity</title><source>PubMed (Medline)</source><source>JSTOR Archival Journals and Primary Sources Collection</source><creator>Smith, Gregory P. ; Fraccia, Tommaso P. ; Todisco, Marco ; Zanchetta, Giuliano ; Zhu, Chenhui ; Hayden, Emily ; Bellini, Tommaso ; Clark, Noel A.</creator><creatorcontrib>Smith, Gregory P. ; Fraccia, Tommaso P. ; Todisco, Marco ; Zanchetta, Giuliano ; Zhu, Chenhui ; Hayden, Emily ; Bellini, Tommaso ; Clark, Noel A. ; Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)</creatorcontrib><description>We demonstrate that nucleic acid (NA) mononucleotide triphosphates (dNTPs and rNTPs), at sufficiently high concentration and low temperature in aqueous solution, can exhibit a phase transition in which chromonic columnar liquid crystal ordering spontaneously appears. Remarkably, this polymer-free state exhibits, in a self-assembly of NA monomers, the key structural elements of biological nucleic acids, including: long-ranged duplex stacking of base pairs, complementarity-dependent partitioning of molecules, and Watson–Crick selectivity, such that, among all solutions of adenosine, cytosine, guanine, and thymine NTPs and their binary mixtures, duplex columnar ordering is most stable in the A-T and C-G combinations.</description><identifier>ISSN: 0027-8424</identifier><identifier>EISSN: 1091-6490</identifier><identifier>DOI: 10.1073/pnas.1721369115</identifier><identifier>PMID: 29967169</identifier><language>eng</language><publisher>United States: National Academy of Sciences</publisher><subject>Adenosine ; Adenosine triphosphate ; Aqueous solutions ; Base pairs ; Base stacking ; BASIC BIOLOGICAL SCIENCES ; Binary mixtures ; Biological Sciences ; Complementarity ; Cytosine ; double helix ; Guanine ; liquid crystal ; Liquid crystal polymers ; Liquid crystals ; Low temperature ; Low temperature physics ; Monomers ; nucleic acid ; Nucleic acids ; nucleoside triphosphate ; Phase transitions ; Physical Sciences ; PNAS Plus ; prebiotic evolution ; Science & Technology - Other Topics ; Selectivity ; Self-assembly ; Structural members ; Thymine</subject><ispartof>Proceedings of the National Academy of Sciences - PNAS, 2018-08, Vol.115 (33), p.E7658-E7664</ispartof><rights>Volumes 1–89 and 106–115, copyright as a collective work only; author(s) retains copyright to individual articles</rights><rights>Copyright © 2018 the Author(s). Published by PNAS.</rights><rights>Copyright National Academy of Sciences Aug 14, 2018</rights><rights>Copyright © 2018 the Author(s). Published by PNAS. 2018</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c536t-175f136c1420a56e83c8d1d5190639a7f42450f65a4efa37d61aef2b0260c3733</citedby><cites>FETCH-LOGICAL-c536t-175f136c1420a56e83c8d1d5190639a7f42450f65a4efa37d61aef2b0260c3733</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/26530263$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/26530263$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>230,315,733,786,790,891,27957,27958,53827,53829,58593,58826</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/29967169$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink><backlink>$$Uhttps://www.osti.gov/servlets/purl/1625012$$D View this record in Osti.gov$$Hfree_for_read</backlink></links><search><creatorcontrib>Smith, Gregory P.</creatorcontrib><creatorcontrib>Fraccia, Tommaso P.</creatorcontrib><creatorcontrib>Todisco, Marco</creatorcontrib><creatorcontrib>Zanchetta, Giuliano</creatorcontrib><creatorcontrib>Zhu, Chenhui</creatorcontrib><creatorcontrib>Hayden, Emily</creatorcontrib><creatorcontrib>Bellini, Tommaso</creatorcontrib><creatorcontrib>Clark, Noel A.</creatorcontrib><creatorcontrib>Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)</creatorcontrib><title>Backbone-free duplex-stacked monomer nucleic acids exhibiting Watson–Crick selectivity</title><title>Proceedings of the National Academy of Sciences - PNAS</title><addtitle>Proc Natl Acad Sci U S A</addtitle><description>We demonstrate that nucleic acid (NA) mononucleotide triphosphates (dNTPs and rNTPs), at sufficiently high concentration and low temperature in aqueous solution, can exhibit a phase transition in which chromonic columnar liquid crystal ordering spontaneously appears. Remarkably, this polymer-free state exhibits, in a self-assembly of NA monomers, the key structural elements of biological nucleic acids, including: long-ranged duplex stacking of base pairs, complementarity-dependent partitioning of molecules, and Watson–Crick selectivity, such that, among all solutions of adenosine, cytosine, guanine, and thymine NTPs and their binary mixtures, duplex columnar ordering is most stable in the A-T and C-G combinations.</description><subject>Adenosine</subject><subject>Adenosine triphosphate</subject><subject>Aqueous solutions</subject><subject>Base pairs</subject><subject>Base stacking</subject><subject>BASIC BIOLOGICAL SCIENCES</subject><subject>Binary mixtures</subject><subject>Biological Sciences</subject><subject>Complementarity</subject><subject>Cytosine</subject><subject>double helix</subject><subject>Guanine</subject><subject>liquid crystal</subject><subject>Liquid crystal polymers</subject><subject>Liquid crystals</subject><subject>Low temperature</subject><subject>Low temperature physics</subject><subject>Monomers</subject><subject>nucleic acid</subject><subject>Nucleic acids</subject><subject>nucleoside triphosphate</subject><subject>Phase transitions</subject><subject>Physical Sciences</subject><subject>PNAS Plus</subject><subject>prebiotic evolution</subject><subject>Science & Technology - Other Topics</subject><subject>Selectivity</subject><subject>Self-assembly</subject><subject>Structural members</subject><subject>Thymine</subject><issn>0027-8424</issn><issn>1091-6490</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2018</creationdate><recordtype>article</recordtype><recordid>eNpdkb9uFDEQxi0EIkegpgKtoKHZxGOv7XWDBCf-SZFoQNBZPu9szpc9-2J7o6TjHXhDngRHFxKgGsnz8zffzEfIU6BHQBU_3gWbj0Ax4FIDiHtkAVRDKztN75MFpUy1fce6A_Io5w2lVIuePiQHTGupQOoF-f7WurNVDNiOCbEZ5t2El20u9RWHZhtD3GJqwuwm9K6xzg-5wcu1X_niw2nzzZYcw68fP5fJu7Mm44Su-Atfrh6TB6OdMj65qYfk6_t3X5Yf25PPHz4t35y0TnBZWlBirOYddIxaIbHnrh9gEKCp5NqqsboXdJTCdjhargYJFke2okxSxxXnh-T1Xnc3r7Y4OAwl2cnskt_adGWi9ebfTvBrcxovjKRa931fBV7sBWIu3mTnC7q1iyHUTQxIJiiwCr26mZLi-Yy5mK3PDqfJBoxzNqy6VaAV0Iq-_A_dxDmFeoNKaaUV61lXqeM95VLMOeF46xiouY7WXEdr7qKtP57_vegt_yfLCjzbA5tcYrrrS8HrtTj_DXJTqn8</recordid><startdate>20180814</startdate><enddate>20180814</enddate><creator>Smith, Gregory P.</creator><creator>Fraccia, Tommaso P.</creator><creator>Todisco, Marco</creator><creator>Zanchetta, Giuliano</creator><creator>Zhu, Chenhui</creator><creator>Hayden, Emily</creator><creator>Bellini, Tommaso</creator><creator>Clark, Noel A.</creator><general>National Academy of Sciences</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QG</scope><scope>7QL</scope><scope>7QP</scope><scope>7QR</scope><scope>7SN</scope><scope>7SS</scope><scope>7T5</scope><scope>7TK</scope><scope>7TM</scope><scope>7TO</scope><scope>7U9</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope><scope>OIOZB</scope><scope>OTOTI</scope><scope>5PM</scope></search><sort><creationdate>20180814</creationdate><title>Backbone-free duplex-stacked monomer nucleic acids exhibiting Watson–Crick selectivity</title><author>Smith, Gregory P. ; Fraccia, Tommaso P. ; Todisco, Marco ; Zanchetta, Giuliano ; Zhu, Chenhui ; Hayden, Emily ; Bellini, Tommaso ; Clark, Noel A.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c536t-175f136c1420a56e83c8d1d5190639a7f42450f65a4efa37d61aef2b0260c3733</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2018</creationdate><topic>Adenosine</topic><topic>Adenosine triphosphate</topic><topic>Aqueous solutions</topic><topic>Base pairs</topic><topic>Base stacking</topic><topic>BASIC BIOLOGICAL SCIENCES</topic><topic>Binary mixtures</topic><topic>Biological Sciences</topic><topic>Complementarity</topic><topic>Cytosine</topic><topic>double helix</topic><topic>Guanine</topic><topic>liquid crystal</topic><topic>Liquid crystal polymers</topic><topic>Liquid crystals</topic><topic>Low temperature</topic><topic>Low temperature physics</topic><topic>Monomers</topic><topic>nucleic acid</topic><topic>Nucleic acids</topic><topic>nucleoside triphosphate</topic><topic>Phase transitions</topic><topic>Physical Sciences</topic><topic>PNAS Plus</topic><topic>prebiotic evolution</topic><topic>Science & Technology - Other Topics</topic><topic>Selectivity</topic><topic>Self-assembly</topic><topic>Structural members</topic><topic>Thymine</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Smith, Gregory P.</creatorcontrib><creatorcontrib>Fraccia, Tommaso P.</creatorcontrib><creatorcontrib>Todisco, Marco</creatorcontrib><creatorcontrib>Zanchetta, Giuliano</creatorcontrib><creatorcontrib>Zhu, Chenhui</creatorcontrib><creatorcontrib>Hayden, Emily</creatorcontrib><creatorcontrib>Bellini, Tommaso</creatorcontrib><creatorcontrib>Clark, Noel A.</creatorcontrib><creatorcontrib>Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>Animal Behavior Abstracts</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Chemoreception Abstracts</collection><collection>Ecology Abstracts</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Immunology Abstracts</collection><collection>Neurosciences Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Oncogenes and Growth Factors Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><collection>OSTI.GOV - Hybrid</collection><collection>OSTI.GOV</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Proceedings of the National Academy of Sciences - PNAS</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Smith, Gregory P.</au><au>Fraccia, Tommaso P.</au><au>Todisco, Marco</au><au>Zanchetta, Giuliano</au><au>Zhu, Chenhui</au><au>Hayden, Emily</au><au>Bellini, Tommaso</au><au>Clark, Noel A.</au><aucorp>Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)</aucorp><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Backbone-free duplex-stacked monomer nucleic acids exhibiting Watson–Crick selectivity</atitle><jtitle>Proceedings of the National Academy of Sciences - PNAS</jtitle><addtitle>Proc Natl Acad Sci U S A</addtitle><date>2018-08-14</date><risdate>2018</risdate><volume>115</volume><issue>33</issue><spage>E7658</spage><epage>E7664</epage><pages>E7658-E7664</pages><issn>0027-8424</issn><eissn>1091-6490</eissn><notes>ObjectType-Article-1</notes><notes>SourceType-Scholarly Journals-1</notes><notes>ObjectType-Feature-2</notes><notes>content type line 23</notes><notes>AC02-05CH11231</notes><notes>USDOE Office of Science (SC)</notes><notes>Reviewers: C.R.S., University of California, Santa Barbara; and A.G.Y., University of Pennsylvania.</notes><notes>Contributed by Noel A. Clark, May 31, 2018 (sent for review December 11, 2017; reviewed by Cyrus R. Safinya and Arjun G. Yodh)</notes><notes>1G.P.S. and T.P.F. contributed equally to this work.</notes><notes>Author contributions: G.P.S., T.P.F., M.T., G.Z., T.B., and N.A.C. designed research; G.P.S., T.P.F., M.T., G.Z., C.Z., E.H., T.B., and N.A.C. performed research; G.P.S., T.P.F., M.T., G.Z., C.Z., E.H., T.B., and N.A.C. analyzed data; and G.P.S., T.P.F., T.B., and N.A.C. wrote the paper.</notes><abstract>We demonstrate that nucleic acid (NA) mononucleotide triphosphates (dNTPs and rNTPs), at sufficiently high concentration and low temperature in aqueous solution, can exhibit a phase transition in which chromonic columnar liquid crystal ordering spontaneously appears. Remarkably, this polymer-free state exhibits, in a self-assembly of NA monomers, the key structural elements of biological nucleic acids, including: long-ranged duplex stacking of base pairs, complementarity-dependent partitioning of molecules, and Watson–Crick selectivity, such that, among all solutions of adenosine, cytosine, guanine, and thymine NTPs and their binary mixtures, duplex columnar ordering is most stable in the A-T and C-G combinations.</abstract><cop>United States</cop><pub>National Academy of Sciences</pub><pmid>29967169</pmid><doi>10.1073/pnas.1721369115</doi><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0027-8424 |
| ispartof | Proceedings of the National Academy of Sciences - PNAS, 2018-08, Vol.115 (33), p.E7658-E7664 |
| issn | 0027-8424 1091-6490 |
| language | eng |
| recordid | cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_6099888 |
| source | PubMed (Medline); JSTOR Archival Journals and Primary Sources Collection |
| subjects | Adenosine Adenosine triphosphate Aqueous solutions Base pairs Base stacking BASIC BIOLOGICAL SCIENCES Binary mixtures Biological Sciences Complementarity Cytosine double helix Guanine liquid crystal Liquid crystal polymers Liquid crystals Low temperature Low temperature physics Monomers nucleic acid Nucleic acids nucleoside triphosphate Phase transitions Physical Sciences PNAS Plus prebiotic evolution Science & Technology - Other Topics Selectivity Self-assembly Structural members Thymine |
| title | Backbone-free duplex-stacked monomer nucleic acids exhibiting Watson–Crick selectivity |
| url | http://sfxeu10.hosted.exlibrisgroup.com/loughborough?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-09-22T23%3A34%3A52IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-jstor_pubme&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Backbone-free%20duplex-stacked%20monomer%20nucleic%20acids%20exhibiting%20Watson%E2%80%93Crick%20selectivity&rft.jtitle=Proceedings%20of%20the%20National%20Academy%20of%20Sciences%20-%20PNAS&rft.au=Smith,%20Gregory%20P.&rft.aucorp=Lawrence%20Berkeley%20National%20Laboratory%20(LBNL),%20Berkeley,%20CA%20(United%20States)&rft.date=2018-08-14&rft.volume=115&rft.issue=33&rft.spage=E7658&rft.epage=E7664&rft.pages=E7658-E7664&rft.issn=0027-8424&rft.eissn=1091-6490&rft_id=info:doi/10.1073/pnas.1721369115&rft_dat=%3Cjstor_pubme%3E26530263%3C/jstor_pubme%3E%3Cgrp_id%3Ecdi_FETCH-LOGICAL-c536t-175f136c1420a56e83c8d1d5190639a7f42450f65a4efa37d61aef2b0260c3733%3C/grp_id%3E%3Coa%3E%3C/oa%3E%3Curl%3E%3C/url%3E&rft_id=info:oai/&rft_pqid=2097972824&rft_id=info:pmid/29967169&rft_jstor_id=26530263&rfr_iscdi=true |