Biphasic regulation of tumorigenesis by PTK7 expression level in esophageal squamous cell carcinoma

Protein tyrosine kinase 7 (PTK7), also known as colon carcinoma kinase 4 (CCK-4), is a member of the catalytically defective receptor protein tyrosine kinase family and is upregulated in various cancers, where it is known to act as either an oncoprotein or a tumor suppressor. To understand the contr...

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Published in:Scientific reports 2018-06, Vol.8 (1), p.8519-9, Article 8519
Main Authors: Shin, Won-Sik, Gim, Jungsoo, Won, Sungho, Lee, Seung-Taek
Format: Article
Language:English
Subjects:
AKT protein
Cell migration
Cell proliferation
Cholecystokinin
Colon
Esophageal cancer
Esophagus
Extracellular signal-regulated kinase
Genomes
Kinases
mRNA
Phosphorylation
Protein-tyrosine kinase
Squamous cell carcinoma
Tumor suppressor genes
Tumorigenesis
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creator Shin, Won-Sik
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Won, Sungho
Lee, Seung-Taek
description Protein tyrosine kinase 7 (PTK7), also known as colon carcinoma kinase 4 (CCK-4), is a member of the catalytically defective receptor protein tyrosine kinase family and is upregulated in various cancers, where it is known to act as either an oncoprotein or a tumor suppressor. To understand the contrasting roles of PTK7 in tumorigenesis, we analyzed the tumorigenic characteristics of esophageal squamous cell carcinoma (ESCC) cells with low levels of endogenous PTK7 expression (TE-5 and TE-14 cells) and high levels of expression (TE-6 and TE-10 cells) after transfections with a PTK7 expression vector. PTK7 overexpression increased the proliferation of TE-5 and TE-14 cells but decreased the proliferation of TE-6 and TE-10 cells. In the ESCC cells, proliferation, migration, and invasion were initially increased and then decreased according to PTK7 expression levels, which were mirrored by initial increases and then decreases in the tyrosine phosphorylation of cellular proteins and phosphorylation of Src, Akt, and ERK. In ESCC patients included in The Cancer Genome Atlas database, those with higher PTK7 mRNA levels had a longer overall survival and lower relative risk than those with lower PTK7 mRNA levels. These results demonstrate that PTK7 biphasically regulates tumorigenesis in ESCC.
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subjects AKT protein
Cell migration
Cell proliferation
Cholecystokinin
Colon
Esophageal cancer
Esophagus
Extracellular signal-regulated kinase
Genomes
Kinases
mRNA
Phosphorylation
Protein-tyrosine kinase
Squamous cell carcinoma
Tumor suppressor genes
Tumorigenesis
title Biphasic regulation of tumorigenesis by PTK7 expression level in esophageal squamous cell carcinoma
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