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Establishment and Characterization of Primary Cultures from Iranian Oral Squamous Cell Carcinoma Patients by Enzymatic Method and Explant Culture
Oral Squamous Cell Carcinoma (OSCC) is the most frequent oral cancer worldwide. It is known as the eighth most common cancer in men and as the fifth most common cancer in women. Cytogenetic and biochemical studies in recent decades have emphasized the necessity of providing an appropriate tool for s...
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Published in: | Journal of dentistry (Tehran, Iran) Iran), 2017-07, Vol.14 (4), p.191-202 |
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creator | Ganjibakhsh, Meysam Aminishakib, Pouyan Farzaneh, Parvaneh Karimi, Abbas Fazeli, Seyed Abolhassan Shahzadeh Rajabi, Moones Nasimian, Ahmad Naini, Fereshteh Baghai Rahmati, Hedieh Gohari, Neda Sadat Mohebali, Nazanin Asadi, Masoumeh Gorji, Zahra Elyasi Izadpanah, Mehrnaz Moghanjoghi, Shiva Mohamadi Ashouri, Sepideh |
description | Oral Squamous Cell Carcinoma (OSCC) is the most frequent oral cancer worldwide. It is known as the eighth most common cancer in men and as the fifth most common cancer in women. Cytogenetic and biochemical studies in recent decades have emphasized the necessity of providing an appropriate tool for such researches. Cancer cell culture is a useful tool for investigations on biochemical, genetic, molecular and immunological characteristics of different cancers, including oral cancer. Here, we explain the establishment process of five primary oral cancer cells derived from an Iranian population.
The specimens were obtained from five oral cancer patients. Enzymatic, explant culture and magnetic-activated cell sorting (MACS) methods were used for cell isolation. After quality control tests, characterization and authentication of primary oral cancer cells were performed by short tandem repeats (STR) profiling, chromosome analysis, species identification, and monitoring the growth, morphology and the expression of CD326 and CD133 markers.
Five primary oral cancer cells were established from an Iranian population. The flow cytometry results showed that the isolated cells were positive for CD326 and CD133 markers. Furthermore, the cells were free from mycoplasma, bacterial and fungal contamination. No misidentified or cross-contaminated cells were detected by STR analysis.
Human primary oral cancer cells provide an extremely useful platform for studying carcinogenesis pathways of oral cancer in Iranian population. They may be helpful in explaining the ethnic differences in cancer biology and the individuality in anticancer drug response in future studies. |
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The specimens were obtained from five oral cancer patients. Enzymatic, explant culture and magnetic-activated cell sorting (MACS) methods were used for cell isolation. After quality control tests, characterization and authentication of primary oral cancer cells were performed by short tandem repeats (STR) profiling, chromosome analysis, species identification, and monitoring the growth, morphology and the expression of CD326 and CD133 markers.
Five primary oral cancer cells were established from an Iranian population. The flow cytometry results showed that the isolated cells were positive for CD326 and CD133 markers. Furthermore, the cells were free from mycoplasma, bacterial and fungal contamination. No misidentified or cross-contaminated cells were detected by STR analysis.
Human primary oral cancer cells provide an extremely useful platform for studying carcinogenesis pathways of oral cancer in Iranian population. They may be helpful in explaining the ethnic differences in cancer biology and the individuality in anticancer drug response in future studies.</description><identifier>ISSN: 1735-2150</identifier><identifier>EISSN: 2008-2185</identifier><identifier>PMID: 29285029</identifier><language>eng</language><publisher>Iran: Tehran University of Medical Sciences</publisher><subject>Original</subject><ispartof>Journal of dentistry (Tehran, Iran), 2017-07, Vol.14 (4), p.191-202</ispartof><rights>Copyright© Dental Research Center, Tehran University of Medical Sciences 2017</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC5745223/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC5745223/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,315,733,786,790,891,53827,53829</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/29285029$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Ganjibakhsh, Meysam</creatorcontrib><creatorcontrib>Aminishakib, Pouyan</creatorcontrib><creatorcontrib>Farzaneh, Parvaneh</creatorcontrib><creatorcontrib>Karimi, Abbas</creatorcontrib><creatorcontrib>Fazeli, Seyed Abolhassan Shahzadeh</creatorcontrib><creatorcontrib>Rajabi, Moones</creatorcontrib><creatorcontrib>Nasimian, Ahmad</creatorcontrib><creatorcontrib>Naini, Fereshteh Baghai</creatorcontrib><creatorcontrib>Rahmati, Hedieh</creatorcontrib><creatorcontrib>Gohari, Neda Sadat</creatorcontrib><creatorcontrib>Mohebali, Nazanin</creatorcontrib><creatorcontrib>Asadi, Masoumeh</creatorcontrib><creatorcontrib>Gorji, Zahra Elyasi</creatorcontrib><creatorcontrib>Izadpanah, Mehrnaz</creatorcontrib><creatorcontrib>Moghanjoghi, Shiva Mohamadi</creatorcontrib><creatorcontrib>Ashouri, Sepideh</creatorcontrib><title>Establishment and Characterization of Primary Cultures from Iranian Oral Squamous Cell Carcinoma Patients by Enzymatic Method and Explant Culture</title><title>Journal of dentistry (Tehran, Iran)</title><addtitle>J Dent (Tehran)</addtitle><description>Oral Squamous Cell Carcinoma (OSCC) is the most frequent oral cancer worldwide. It is known as the eighth most common cancer in men and as the fifth most common cancer in women. Cytogenetic and biochemical studies in recent decades have emphasized the necessity of providing an appropriate tool for such researches. Cancer cell culture is a useful tool for investigations on biochemical, genetic, molecular and immunological characteristics of different cancers, including oral cancer. Here, we explain the establishment process of five primary oral cancer cells derived from an Iranian population.
The specimens were obtained from five oral cancer patients. Enzymatic, explant culture and magnetic-activated cell sorting (MACS) methods were used for cell isolation. After quality control tests, characterization and authentication of primary oral cancer cells were performed by short tandem repeats (STR) profiling, chromosome analysis, species identification, and monitoring the growth, morphology and the expression of CD326 and CD133 markers.
Five primary oral cancer cells were established from an Iranian population. The flow cytometry results showed that the isolated cells were positive for CD326 and CD133 markers. Furthermore, the cells were free from mycoplasma, bacterial and fungal contamination. No misidentified or cross-contaminated cells were detected by STR analysis.
Human primary oral cancer cells provide an extremely useful platform for studying carcinogenesis pathways of oral cancer in Iranian population. They may be helpful in explaining the ethnic differences in cancer biology and the individuality in anticancer drug response in future studies.</description><subject>Original</subject><issn>1735-2150</issn><issn>2008-2185</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2017</creationdate><recordtype>article</recordtype><recordid>eNpVkN1KxDAQhYMo7rr6CpIXWEjTpm1uBClVF1Z2Qb0uk5_aSJvUtBV338I3NugqejUzzMx35swRmlNC8iWNcnaM5lEWs5AzMkNnw_BCCEt5xk7RjHKaM0L5HH2UwwiiNUPTaTtisAoXDXiQo_ZmD6NxFrsab73pwO9wMbXj5PWAa-86vPJgDVi88dDih9cJOjcNuNBtiwvw0ljXAd4GSEAPWOxwafe7LtQS3-uxcepLr3zvWwjaB_Y5OqmhHfTFIS7Q0035WNwt15vbVXG9XvZRFMVL4CoGIDLKtc5kTmpIBAGVMJXEXKaUKCB5wsOYgFSkIClJlFK85ilTIhHxAl19c_tJdFrJcGOwUfXfTisHpvrfsaapnt1bxbKEURoHwOVfwO_mz3PjTxpufJU</recordid><startdate>201707</startdate><enddate>201707</enddate><creator>Ganjibakhsh, Meysam</creator><creator>Aminishakib, Pouyan</creator><creator>Farzaneh, Parvaneh</creator><creator>Karimi, Abbas</creator><creator>Fazeli, Seyed Abolhassan Shahzadeh</creator><creator>Rajabi, Moones</creator><creator>Nasimian, Ahmad</creator><creator>Naini, Fereshteh Baghai</creator><creator>Rahmati, Hedieh</creator><creator>Gohari, Neda Sadat</creator><creator>Mohebali, Nazanin</creator><creator>Asadi, Masoumeh</creator><creator>Gorji, Zahra Elyasi</creator><creator>Izadpanah, Mehrnaz</creator><creator>Moghanjoghi, Shiva Mohamadi</creator><creator>Ashouri, Sepideh</creator><general>Tehran University of Medical Sciences</general><scope>NPM</scope><scope>5PM</scope></search><sort><creationdate>201707</creationdate><title>Establishment and Characterization of Primary Cultures from Iranian Oral Squamous Cell Carcinoma Patients by Enzymatic Method and Explant Culture</title><author>Ganjibakhsh, Meysam ; Aminishakib, Pouyan ; Farzaneh, Parvaneh ; Karimi, Abbas ; Fazeli, Seyed Abolhassan Shahzadeh ; Rajabi, Moones ; Nasimian, Ahmad ; Naini, Fereshteh Baghai ; Rahmati, Hedieh ; Gohari, Neda Sadat ; Mohebali, Nazanin ; Asadi, Masoumeh ; Gorji, Zahra Elyasi ; Izadpanah, Mehrnaz ; Moghanjoghi, Shiva Mohamadi ; Ashouri, Sepideh</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-p1113-a9d3aa0c18ee7c80fa4b0ad45d439c620da0849a9dba6b6ac204ddd9f965db4b3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2017</creationdate><topic>Original</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ganjibakhsh, Meysam</creatorcontrib><creatorcontrib>Aminishakib, Pouyan</creatorcontrib><creatorcontrib>Farzaneh, Parvaneh</creatorcontrib><creatorcontrib>Karimi, Abbas</creatorcontrib><creatorcontrib>Fazeli, Seyed Abolhassan Shahzadeh</creatorcontrib><creatorcontrib>Rajabi, Moones</creatorcontrib><creatorcontrib>Nasimian, Ahmad</creatorcontrib><creatorcontrib>Naini, Fereshteh Baghai</creatorcontrib><creatorcontrib>Rahmati, Hedieh</creatorcontrib><creatorcontrib>Gohari, Neda Sadat</creatorcontrib><creatorcontrib>Mohebali, Nazanin</creatorcontrib><creatorcontrib>Asadi, Masoumeh</creatorcontrib><creatorcontrib>Gorji, Zahra Elyasi</creatorcontrib><creatorcontrib>Izadpanah, Mehrnaz</creatorcontrib><creatorcontrib>Moghanjoghi, Shiva Mohamadi</creatorcontrib><creatorcontrib>Ashouri, Sepideh</creatorcontrib><collection>PubMed</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Journal of dentistry (Tehran, Iran)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ganjibakhsh, Meysam</au><au>Aminishakib, Pouyan</au><au>Farzaneh, Parvaneh</au><au>Karimi, Abbas</au><au>Fazeli, Seyed Abolhassan Shahzadeh</au><au>Rajabi, Moones</au><au>Nasimian, Ahmad</au><au>Naini, Fereshteh Baghai</au><au>Rahmati, Hedieh</au><au>Gohari, Neda Sadat</au><au>Mohebali, Nazanin</au><au>Asadi, Masoumeh</au><au>Gorji, Zahra Elyasi</au><au>Izadpanah, Mehrnaz</au><au>Moghanjoghi, Shiva Mohamadi</au><au>Ashouri, Sepideh</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Establishment and Characterization of Primary Cultures from Iranian Oral Squamous Cell Carcinoma Patients by Enzymatic Method and Explant Culture</atitle><jtitle>Journal of dentistry (Tehran, Iran)</jtitle><addtitle>J Dent (Tehran)</addtitle><date>2017-07</date><risdate>2017</risdate><volume>14</volume><issue>4</issue><spage>191</spage><epage>202</epage><pages>191-202</pages><issn>1735-2150</issn><eissn>2008-2185</eissn><abstract>Oral Squamous Cell Carcinoma (OSCC) is the most frequent oral cancer worldwide. It is known as the eighth most common cancer in men and as the fifth most common cancer in women. Cytogenetic and biochemical studies in recent decades have emphasized the necessity of providing an appropriate tool for such researches. Cancer cell culture is a useful tool for investigations on biochemical, genetic, molecular and immunological characteristics of different cancers, including oral cancer. Here, we explain the establishment process of five primary oral cancer cells derived from an Iranian population.
The specimens were obtained from five oral cancer patients. Enzymatic, explant culture and magnetic-activated cell sorting (MACS) methods were used for cell isolation. After quality control tests, characterization and authentication of primary oral cancer cells were performed by short tandem repeats (STR) profiling, chromosome analysis, species identification, and monitoring the growth, morphology and the expression of CD326 and CD133 markers.
Five primary oral cancer cells were established from an Iranian population. The flow cytometry results showed that the isolated cells were positive for CD326 and CD133 markers. Furthermore, the cells were free from mycoplasma, bacterial and fungal contamination. No misidentified or cross-contaminated cells were detected by STR analysis.
Human primary oral cancer cells provide an extremely useful platform for studying carcinogenesis pathways of oral cancer in Iranian population. They may be helpful in explaining the ethnic differences in cancer biology and the individuality in anticancer drug response in future studies.</abstract><cop>Iran</cop><pub>Tehran University of Medical Sciences</pub><pmid>29285029</pmid><tpages>12</tpages><oa>free_for_read</oa></addata></record> |
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title | Establishment and Characterization of Primary Cultures from Iranian Oral Squamous Cell Carcinoma Patients by Enzymatic Method and Explant Culture |
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