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Optically Encoded Semiconducting Polymer Dots with Single-Wavelength Excitation for Barcoding and Tracking of Single Cells
Multiplexed optical encoding is emerging as a powerful technique for high-throughput cellular analysis and molecular assays. Most of the developed optical barcodes, however, either suffer from large particle size or are incompatible with most commercial optical instruments. Here, a new type of nanos...
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| Published in: | Analytical chemistry (Washington) 2017-06, Vol.89 (11), p.6232-6238 |
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| Main Authors: | , , , , , , |
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| cited_by | cdi_FETCH-LOGICAL-a477t-6dba782e3b9ec18f2d75c028463d49c8961a99188a9afc866f5150edece88c763 |
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| cites | cdi_FETCH-LOGICAL-a477t-6dba782e3b9ec18f2d75c028463d49c8961a99188a9afc866f5150edece88c763 |
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| container_issue | 11 |
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| creator | Kuo, Chun-Ting Peng, Hong-Shang Rong, Yu Yu, Jiangbo Sun, Wei Fujimoto, Bryant Chiu, Daniel T |
| description | Multiplexed optical encoding is emerging as a powerful technique for high-throughput cellular analysis and molecular assays. Most of the developed optical barcodes, however, either suffer from large particle size or are incompatible with most commercial optical instruments. Here, a new type of nanoscale fluorescent barcode (Pdot barcodes) was prepared from semiconducting polymers. The Pdot barcodes possess the merits of small size (∼20 nm in diameter), narrow emission bands (full-width-at-half-maximum (fwhm) of 30–40 nm), three-color emissions (blue, green, and red) under single-wavelength excitation, a high brightness, good pH and thermal stability, and efficient cellular uptake. The Pdot barcodes were prepared using a three-color and six-intensity encoding strategy; for ratiometric readout of the barcodes, one of the colors might be used as an internal reference. We used the Pdot barcodes to label 20 sets of cancer cells and then distinguished and identified each set based on the Pdot barcodes using flow cytometry. We also monitored and tracked single cells labeled with different Pdot barcodes, even through rounds of cell division. These results suggest Pdot barcodes are strong candidates for discriminating different labeled cell and for long-term cell tracking. |
| doi_str_mv | 10.1021/acs.analchem.7b01214 |
| format | article |
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Most of the developed optical barcodes, however, either suffer from large particle size or are incompatible with most commercial optical instruments. Here, a new type of nanoscale fluorescent barcode (Pdot barcodes) was prepared from semiconducting polymers. The Pdot barcodes possess the merits of small size (∼20 nm in diameter), narrow emission bands (full-width-at-half-maximum (fwhm) of 30–40 nm), three-color emissions (blue, green, and red) under single-wavelength excitation, a high brightness, good pH and thermal stability, and efficient cellular uptake. The Pdot barcodes were prepared using a three-color and six-intensity encoding strategy; for ratiometric readout of the barcodes, one of the colors might be used as an internal reference. We used the Pdot barcodes to label 20 sets of cancer cells and then distinguished and identified each set based on the Pdot barcodes using flow cytometry. We also monitored and tracked single cells labeled with different Pdot barcodes, even through rounds of cell division. These results suggest Pdot barcodes are strong candidates for discriminating different labeled cell and for long-term cell tracking.</description><identifier>ISSN: 0003-2700</identifier><identifier>EISSN: 1520-6882</identifier><identifier>DOI: 10.1021/acs.analchem.7b01214</identifier><identifier>PMID: 28499337</identifier><language>eng</language><publisher>United States: American Chemical Society</publisher><subject>Bar codes ; Boron Compounds - chemistry ; Brightness ; Cancer ; Cell division ; Cells ; Chemistry ; Color ; Cytometry ; Division ; Emissions ; Excitation ; Flow cytometry ; Fluorenes - chemistry ; Fluorescence ; Fluorescent Dyes - chemical synthesis ; Fluorescent Dyes - chemistry ; Humans ; Hydrogen-Ion Concentration ; MCF-7 Cells ; Molecular Structure ; Multiplexing ; Optical instruments ; Optical Phenomena ; Particle Size ; pH effects ; Polymers ; Polymers - chemical synthesis ; Polymers - chemistry ; Quantum Dots - chemistry ; Semiconductors ; Single-Cell Analysis ; Surface Properties ; Temperature ; Thermal stability ; Tracking ; Tumor Cells, Cultured ; Wavelength</subject><ispartof>Analytical chemistry (Washington), 2017-06, Vol.89 (11), p.6232-6238</ispartof><rights>Copyright © 2017 American Chemical Society</rights><rights>Copyright American Chemical Society Jun 6, 2017</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-a477t-6dba782e3b9ec18f2d75c028463d49c8961a99188a9afc866f5150edece88c763</citedby><cites>FETCH-LOGICAL-a477t-6dba782e3b9ec18f2d75c028463d49c8961a99188a9afc866f5150edece88c763</cites><orcidid>0000-0003-2964-9578 ; 0000-0003-0634-3867</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>230,315,786,790,891,27957,27958</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/28499337$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Kuo, Chun-Ting</creatorcontrib><creatorcontrib>Peng, Hong-Shang</creatorcontrib><creatorcontrib>Rong, Yu</creatorcontrib><creatorcontrib>Yu, Jiangbo</creatorcontrib><creatorcontrib>Sun, Wei</creatorcontrib><creatorcontrib>Fujimoto, Bryant</creatorcontrib><creatorcontrib>Chiu, Daniel T</creatorcontrib><title>Optically Encoded Semiconducting Polymer Dots with Single-Wavelength Excitation for Barcoding and Tracking of Single Cells</title><title>Analytical chemistry (Washington)</title><addtitle>Anal. Chem</addtitle><description>Multiplexed optical encoding is emerging as a powerful technique for high-throughput cellular analysis and molecular assays. Most of the developed optical barcodes, however, either suffer from large particle size or are incompatible with most commercial optical instruments. Here, a new type of nanoscale fluorescent barcode (Pdot barcodes) was prepared from semiconducting polymers. The Pdot barcodes possess the merits of small size (∼20 nm in diameter), narrow emission bands (full-width-at-half-maximum (fwhm) of 30–40 nm), three-color emissions (blue, green, and red) under single-wavelength excitation, a high brightness, good pH and thermal stability, and efficient cellular uptake. The Pdot barcodes were prepared using a three-color and six-intensity encoding strategy; for ratiometric readout of the barcodes, one of the colors might be used as an internal reference. We used the Pdot barcodes to label 20 sets of cancer cells and then distinguished and identified each set based on the Pdot barcodes using flow cytometry. We also monitored and tracked single cells labeled with different Pdot barcodes, even through rounds of cell division. These results suggest Pdot barcodes are strong candidates for discriminating different labeled cell and for long-term cell tracking.</description><subject>Bar codes</subject><subject>Boron Compounds - chemistry</subject><subject>Brightness</subject><subject>Cancer</subject><subject>Cell division</subject><subject>Cells</subject><subject>Chemistry</subject><subject>Color</subject><subject>Cytometry</subject><subject>Division</subject><subject>Emissions</subject><subject>Excitation</subject><subject>Flow cytometry</subject><subject>Fluorenes - chemistry</subject><subject>Fluorescence</subject><subject>Fluorescent Dyes - chemical synthesis</subject><subject>Fluorescent Dyes - chemistry</subject><subject>Humans</subject><subject>Hydrogen-Ion Concentration</subject><subject>MCF-7 Cells</subject><subject>Molecular Structure</subject><subject>Multiplexing</subject><subject>Optical instruments</subject><subject>Optical Phenomena</subject><subject>Particle Size</subject><subject>pH effects</subject><subject>Polymers</subject><subject>Polymers - chemical synthesis</subject><subject>Polymers - chemistry</subject><subject>Quantum Dots - chemistry</subject><subject>Semiconductors</subject><subject>Single-Cell Analysis</subject><subject>Surface Properties</subject><subject>Temperature</subject><subject>Thermal stability</subject><subject>Tracking</subject><subject>Tumor Cells, Cultured</subject><subject>Wavelength</subject><issn>0003-2700</issn><issn>1520-6882</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2017</creationdate><recordtype>article</recordtype><recordid>eNp9kUtvEzEUhS0EomnhHyBkiQ2bCfa8bG-QIISHVKlILWJp3XjuJC4eO9iTQvrr8ShpeSxYWb7-zrk-OoQ842zOWclfgUlz8ODMBoe5WDFe8voBmfGmZEUrZfmQzBhjVVEKxk7IaUrXjHHOePuYnJSyVqqqxIzcXmxHa8C5PV16Ezrs6CUO1gTf7cxo_Zp-Dm4_YKTvwpjoDztu6GUeOyy-wg069Os8Wf40doTRBk_7EOlbiNlqEoPv6FUE8226hP4opQt0Lj0hj3pwCZ8ezzPy5f3yavGxOL_48Gnx5ryAWoixaLsVCFlitVJouOzLTjSG5QRt1dXKSNVyUIpLCQp6I9u2b3jDsEODUhrRVmfk9cF3u1sN2Bn0YwSnt9EOEPc6gNV_v3i70etwo5tWNU2jssHLo0EM33eYRj3YZHIE8Bh2SXOZ97O6FBP64h_0OuxibilTildCVKqRmaoPlIkhpYj9_Wc401O5Oper78rVx3Kz7PmfQe5Fd21mgB2ASf578f88fwFnhrZJ</recordid><startdate>20170606</startdate><enddate>20170606</enddate><creator>Kuo, Chun-Ting</creator><creator>Peng, Hong-Shang</creator><creator>Rong, Yu</creator><creator>Yu, Jiangbo</creator><creator>Sun, Wei</creator><creator>Fujimoto, Bryant</creator><creator>Chiu, Daniel T</creator><general>American Chemical Society</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QF</scope><scope>7QO</scope><scope>7QQ</scope><scope>7SC</scope><scope>7SE</scope><scope>7SP</scope><scope>7SR</scope><scope>7TA</scope><scope>7TB</scope><scope>7TM</scope><scope>7U5</scope><scope>7U7</scope><scope>7U9</scope><scope>8BQ</scope><scope>8FD</scope><scope>C1K</scope><scope>F28</scope><scope>FR3</scope><scope>H8D</scope><scope>H8G</scope><scope>H94</scope><scope>JG9</scope><scope>JQ2</scope><scope>KR7</scope><scope>L7M</scope><scope>L~C</scope><scope>L~D</scope><scope>P64</scope><scope>7X8</scope><scope>5PM</scope><orcidid>https://orcid.org/0000-0003-2964-9578</orcidid><orcidid>https://orcid.org/0000-0003-0634-3867</orcidid></search><sort><creationdate>20170606</creationdate><title>Optically Encoded Semiconducting Polymer Dots with Single-Wavelength Excitation for Barcoding and Tracking of Single Cells</title><author>Kuo, Chun-Ting ; 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Chem</addtitle><date>2017-06-06</date><risdate>2017</risdate><volume>89</volume><issue>11</issue><spage>6232</spage><epage>6238</epage><pages>6232-6238</pages><issn>0003-2700</issn><eissn>1520-6882</eissn><notes>ObjectType-Article-1</notes><notes>SourceType-Scholarly Journals-1</notes><notes>ObjectType-Feature-2</notes><notes>content type line 23</notes><notes>Daniel T. Chiu: 0000-0003-2964-9578</notes><notes>ORCID</notes><notes>Hong-Shang Peng: 0000-0003-0634-3867</notes><notes>C.-T.K. and H.-S.P. contributed equally to this project.</notes><abstract>Multiplexed optical encoding is emerging as a powerful technique for high-throughput cellular analysis and molecular assays. Most of the developed optical barcodes, however, either suffer from large particle size or are incompatible with most commercial optical instruments. Here, a new type of nanoscale fluorescent barcode (Pdot barcodes) was prepared from semiconducting polymers. The Pdot barcodes possess the merits of small size (∼20 nm in diameter), narrow emission bands (full-width-at-half-maximum (fwhm) of 30–40 nm), three-color emissions (blue, green, and red) under single-wavelength excitation, a high brightness, good pH and thermal stability, and efficient cellular uptake. The Pdot barcodes were prepared using a three-color and six-intensity encoding strategy; for ratiometric readout of the barcodes, one of the colors might be used as an internal reference. We used the Pdot barcodes to label 20 sets of cancer cells and then distinguished and identified each set based on the Pdot barcodes using flow cytometry. We also monitored and tracked single cells labeled with different Pdot barcodes, even through rounds of cell division. These results suggest Pdot barcodes are strong candidates for discriminating different labeled cell and for long-term cell tracking.</abstract><cop>United States</cop><pub>American Chemical Society</pub><pmid>28499337</pmid><doi>10.1021/acs.analchem.7b01214</doi><tpages>7</tpages><orcidid>https://orcid.org/0000-0003-2964-9578</orcidid><orcidid>https://orcid.org/0000-0003-0634-3867</orcidid><oa>free_for_read</oa></addata></record> |
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| source | American Chemical Society:Jisc Collections:American Chemical Society Read & Publish Agreement 2022-2024 (Reading list) |
| subjects | Bar codes Boron Compounds - chemistry Brightness Cancer Cell division Cells Chemistry Color Cytometry Division Emissions Excitation Flow cytometry Fluorenes - chemistry Fluorescence Fluorescent Dyes - chemical synthesis Fluorescent Dyes - chemistry Humans Hydrogen-Ion Concentration MCF-7 Cells Molecular Structure Multiplexing Optical instruments Optical Phenomena Particle Size pH effects Polymers Polymers - chemical synthesis Polymers - chemistry Quantum Dots - chemistry Semiconductors Single-Cell Analysis Surface Properties Temperature Thermal stability Tracking Tumor Cells, Cultured Wavelength |
| title | Optically Encoded Semiconducting Polymer Dots with Single-Wavelength Excitation for Barcoding and Tracking of Single Cells |
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