The Comparatively Proteomic Analysis in Response to Cold Stress in Cassava Plantlets

Cassava ( Manihot esculenta Crantz) is a tropical root crop and sensitive to low temperature. However, it is poorly to know how cassava can modify its metabolism and growth to adapt to cold stress. An investigation aimed at a better understanding of cold-tolerant mechanism of cassava plantlets was c...

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Bibliographic Details
Published in:Plant molecular biology reporter 2016-12, Vol.34 (6), p.1095-1110
Main Authors: An, Feifei, Li, Genghu, Li, Qing X., Li, Kaimian, Carvalho, Luiz J. C. B., Ou, Wenjun, Chen, Songbi
Format: Article
Language:English
Subjects:
Bioinformatics
Biomedical and Life Sciences
Life Sciences
Metabolomics
Original Paper
Plant biology
Plant Breeding/Biotechnology
Plant Sciences
Proteomics
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Summary:Cassava ( Manihot esculenta Crantz) is a tropical root crop and sensitive to low temperature. However, it is poorly to know how cassava can modify its metabolism and growth to adapt to cold stress. An investigation aimed at a better understanding of cold-tolerant mechanism of cassava plantlets was carried out with the approaches of physiology and proteomics in the present study. The principal component analysis of seven physiological characteristics showed that electrolyte leakage (EL), chlorophyll content, and malondialdehyde (MDA) may be the most important physiological indexes for determining cold-resistant abilities of cassava. The genome-wide proteomic analysis showed that 20 differential proteins had the same patterns in the apical expanded leaves of cassava SC8 and Col1046. They were mainly related to photosynthesis, carbon metabolism and energy metabolism, defense, protein synthesis, amino acid metabolism, signal transduction, structure, detoxifying and antioxidant, chaperones, and DNA-binding proteins, in which 40 % were related with photosynthesis. The remarkable variation in photosynthetic activity and expression level of peroxiredoxin is closely linked with expression levels of proteomic profiles. Moreover, analysis of differentially expressed proteins under cold stress is an important step toward further elucidation of mechanisms of cold stress resistance.
ISSN:0735-9640
1572-9818
1556-469X
DOI:10.1007/s11105-016-0987-x