Collagen types I and III in giant papillae of vernal keratoconjunctivitis

AIMS--The objective of this study was to investigate alterations in conjunctival collagen and proteoglycans in the conjunctival giant papillae of patients with vernal keratoconjunctivitis (VKC). METHODS--Tissue samples from tarsal giant papillae of seven eyes from five patients with VKC, and five ta...

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Published in:British journal of ophthalmology 1995-05, Vol.79 (5), p.482-485
Main Authors: Leonardi, A, Abatangelo, G, Cortivo, R, Secchi, A G
Format: Article
Language:English
Subjects:
Adolescent
Adult
Biological and medical sciences
Child
Collagen - analysis
Conjunctiva - pathology
Conjunctivitis, Allergic - metabolism
Conjunctivitis, Allergic - pathology
Diseases of eyelid, conjunctiva and lacrimal tracts
Electrophoresis, Polyacrylamide Gel
Female
Histocytochemistry
Humans
Male
Mast Cells - pathology
Medical sciences
Ophthalmology
Proteoglycans - analysis
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Abatangelo, G
Cortivo, R
Secchi, A G
description AIMS--The objective of this study was to investigate alterations in conjunctival collagen and proteoglycans in the conjunctival giant papillae of patients with vernal keratoconjunctivitis (VKC). METHODS--Tissue samples from tarsal giant papillae of seven eyes from five patients with VKC, and five tarsal conjunctival samples from five normal patients were obtained. Tissues were processed and stained with haematoxylin and eosin, Van Gieson, trichromic Mallory, toluidine blue, Alcian blue, and alkaline Giemsa. Collagen extraction was performed in acetic acid and pepsin, total collagen was quantified using hydroxy-proline levels, and collagen types I and III were analysed by gel electrophoresis (SDS-PAGE). Proteoglycans were quantified using uronic acid levels. RESULTS--Histological evaluation showed a significant increase of mast cells in the epithelium (0/mm2 v 147/mm2, p < 0.01) and in the stroma (5.1/mm2 v 80/mm2, p < 0.01) of VKC patients. Collagen fibres were thicker and arranged irregularly, with the total amount significantly increased. Owing to an increased percentage of type III collagen, the ratio of collagen types I to III was decreased. Proteoglycans were also reduced in VKC samples. CONCLUSION--The well known morphological abnormalities observed in VKC correspond to alterations in the ratio between collagens and proteoglycans, and between different types of collagen. The greatly increased number of mast cells found in these tissues suggests an active role for these cells in the abnormal connective tissue metabolism observed in VKC.
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METHODS--Tissue samples from tarsal giant papillae of seven eyes from five patients with VKC, and five tarsal conjunctival samples from five normal patients were obtained. Tissues were processed and stained with haematoxylin and eosin, Van Gieson, trichromic Mallory, toluidine blue, Alcian blue, and alkaline Giemsa. Collagen extraction was performed in acetic acid and pepsin, total collagen was quantified using hydroxy-proline levels, and collagen types I and III were analysed by gel electrophoresis (SDS-PAGE). Proteoglycans were quantified using uronic acid levels. RESULTS--Histological evaluation showed a significant increase of mast cells in the epithelium (0/mm2 v 147/mm2, p &lt; 0.01) and in the stroma (5.1/mm2 v 80/mm2, p &lt; 0.01) of VKC patients. Collagen fibres were thicker and arranged irregularly, with the total amount significantly increased. Owing to an increased percentage of type III collagen, the ratio of collagen types I to III was decreased. Proteoglycans were also reduced in VKC samples. CONCLUSION--The well known morphological abnormalities observed in VKC correspond to alterations in the ratio between collagens and proteoglycans, and between different types of collagen. The greatly increased number of mast cells found in these tissues suggests an active role for these cells in the abnormal connective tissue metabolism observed in VKC.</description><identifier>ISSN: 0007-1161</identifier><identifier>EISSN: 1468-2079</identifier><identifier>DOI: 10.1136/bjo.79.5.482</identifier><identifier>PMID: 7612563</identifier><identifier>CODEN: BJOPAL</identifier><language>eng</language><publisher>BMA House, Tavistock Square, London, WC1H 9JR: BMJ Publishing Group Ltd</publisher><subject>Adolescent ; Adult ; Biological and medical sciences ; Child ; Collagen - analysis ; Conjunctiva - pathology ; Conjunctivitis, Allergic - metabolism ; Conjunctivitis, Allergic - pathology ; Diseases of eyelid, conjunctiva and lacrimal tracts ; Electrophoresis, Polyacrylamide Gel ; Female ; Histocytochemistry ; Humans ; Male ; Mast Cells - pathology ; Medical sciences ; Ophthalmology ; Proteoglycans - analysis</subject><ispartof>British journal of ophthalmology, 1995-05, Vol.79 (5), p.482-485</ispartof><rights>1995 INIST-CNRS</rights><rights>Copyright BMJ Publishing Group LTD May 1995</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-b4202-750db653423a347817d9dc04d9d59bd9c3c0466f58a4fad5c880b7362a7409663</citedby><cites>FETCH-LOGICAL-b4202-750db653423a347817d9dc04d9d59bd9c3c0466f58a4fad5c880b7362a7409663</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC505140/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC505140/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,315,733,786,790,891,27957,27958,53827,53829</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&amp;idt=3539166$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/7612563$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Leonardi, A</creatorcontrib><creatorcontrib>Abatangelo, G</creatorcontrib><creatorcontrib>Cortivo, R</creatorcontrib><creatorcontrib>Secchi, A G</creatorcontrib><title>Collagen types I and III in giant papillae of vernal keratoconjunctivitis</title><title>British journal of ophthalmology</title><addtitle>Br J Ophthalmol</addtitle><description>AIMS--The objective of this study was to investigate alterations in conjunctival collagen and proteoglycans in the conjunctival giant papillae of patients with vernal keratoconjunctivitis (VKC). METHODS--Tissue samples from tarsal giant papillae of seven eyes from five patients with VKC, and five tarsal conjunctival samples from five normal patients were obtained. Tissues were processed and stained with haematoxylin and eosin, Van Gieson, trichromic Mallory, toluidine blue, Alcian blue, and alkaline Giemsa. Collagen extraction was performed in acetic acid and pepsin, total collagen was quantified using hydroxy-proline levels, and collagen types I and III were analysed by gel electrophoresis (SDS-PAGE). Proteoglycans were quantified using uronic acid levels. RESULTS--Histological evaluation showed a significant increase of mast cells in the epithelium (0/mm2 v 147/mm2, p &lt; 0.01) and in the stroma (5.1/mm2 v 80/mm2, p &lt; 0.01) of VKC patients. Collagen fibres were thicker and arranged irregularly, with the total amount significantly increased. Owing to an increased percentage of type III collagen, the ratio of collagen types I to III was decreased. Proteoglycans were also reduced in VKC samples. CONCLUSION--The well known morphological abnormalities observed in VKC correspond to alterations in the ratio between collagens and proteoglycans, and between different types of collagen. The greatly increased number of mast cells found in these tissues suggests an active role for these cells in the abnormal connective tissue metabolism observed in VKC.</description><subject>Adolescent</subject><subject>Adult</subject><subject>Biological and medical sciences</subject><subject>Child</subject><subject>Collagen - analysis</subject><subject>Conjunctiva - pathology</subject><subject>Conjunctivitis, Allergic - metabolism</subject><subject>Conjunctivitis, Allergic - pathology</subject><subject>Diseases of eyelid, conjunctiva and lacrimal tracts</subject><subject>Electrophoresis, Polyacrylamide Gel</subject><subject>Female</subject><subject>Histocytochemistry</subject><subject>Humans</subject><subject>Male</subject><subject>Mast Cells - pathology</subject><subject>Medical sciences</subject><subject>Ophthalmology</subject><subject>Proteoglycans - analysis</subject><issn>0007-1161</issn><issn>1468-2079</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1995</creationdate><recordtype>article</recordtype><recordid>eNp9kc1v1DAQxS1EVZbCjSuSJRBcyGLHX_GBA9oCjVSBkErFzXIcZ3GatYOdrNr_vl52tSocuNgavd-M3swD4AVGS4wJf9_0YSnkki1pVT4CC0x5VZRIyMdggRASBcYcPwFPU-pzWXIsTsGp4LhknCxAvQrDoNfWw-lutAnWUPsW1nUNnYdrp_0ERz26zFgYOri10esB3tiop2CC72dvJrd1k0vPwEmnh2SfH_4z8OPzp6vVRXH57Uu9-nhZNLREZSEYahvOCC2JJlRUWLSyNYjml8mmlYbkgvOOVZp2umWmqlAjCC-1oEhyTs7Ah_3ccW42tjXWT1EPaoxuo-OdCtqpvxXvfql12CqGGKYo97859Mfwe7ZpUhuXjM0behvmpIQgEjEqMvjqH7AP8279pLAQCBEuSZmpd3vKxJBStN3RCUZql4_K-SghFVM5n4y_fOj-CB8Cyfrrg66T0UMXtTcuHTHCiMR_jlDsMZcme3uUdbxRXBDB1NfrlbpC1-Qcff-pdmPf7vlm0__f4D2uYLL5</recordid><startdate>19950501</startdate><enddate>19950501</enddate><creator>Leonardi, A</creator><creator>Abatangelo, G</creator><creator>Cortivo, R</creator><creator>Secchi, A G</creator><general>BMJ Publishing Group Ltd</general><general>BMJ</general><general>BMJ Publishing Group LTD</general><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>BENPR</scope><scope>BTHHO</scope><scope>CCPQU</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>K9.</scope><scope>M0S</scope><scope>M1P</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>19950501</creationdate><title>Collagen types I and III in giant papillae of vernal keratoconjunctivitis</title><author>Leonardi, A ; 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METHODS--Tissue samples from tarsal giant papillae of seven eyes from five patients with VKC, and five tarsal conjunctival samples from five normal patients were obtained. Tissues were processed and stained with haematoxylin and eosin, Van Gieson, trichromic Mallory, toluidine blue, Alcian blue, and alkaline Giemsa. Collagen extraction was performed in acetic acid and pepsin, total collagen was quantified using hydroxy-proline levels, and collagen types I and III were analysed by gel electrophoresis (SDS-PAGE). Proteoglycans were quantified using uronic acid levels. RESULTS--Histological evaluation showed a significant increase of mast cells in the epithelium (0/mm2 v 147/mm2, p &lt; 0.01) and in the stroma (5.1/mm2 v 80/mm2, p &lt; 0.01) of VKC patients. Collagen fibres were thicker and arranged irregularly, with the total amount significantly increased. Owing to an increased percentage of type III collagen, the ratio of collagen types I to III was decreased. Proteoglycans were also reduced in VKC samples. CONCLUSION--The well known morphological abnormalities observed in VKC correspond to alterations in the ratio between collagens and proteoglycans, and between different types of collagen. The greatly increased number of mast cells found in these tissues suggests an active role for these cells in the abnormal connective tissue metabolism observed in VKC.</abstract><cop>BMA House, Tavistock Square, London, WC1H 9JR</cop><pub>BMJ Publishing Group Ltd</pub><pmid>7612563</pmid><doi>10.1136/bjo.79.5.482</doi><tpages>4</tpages><oa>free_for_read</oa></addata></record>
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identifier ISSN: 0007-1161
ispartof British journal of ophthalmology, 1995-05, Vol.79 (5), p.482-485
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1468-2079
language eng
recordid cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_505140
source PubMed Central; Alma/SFX Local Collection
subjects Adolescent
Adult
Biological and medical sciences
Child
Collagen - analysis
Conjunctiva - pathology
Conjunctivitis, Allergic - metabolism
Conjunctivitis, Allergic - pathology
Diseases of eyelid, conjunctiva and lacrimal tracts
Electrophoresis, Polyacrylamide Gel
Female
Histocytochemistry
Humans
Male
Mast Cells - pathology
Medical sciences
Ophthalmology
Proteoglycans - analysis
title Collagen types I and III in giant papillae of vernal keratoconjunctivitis
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