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Subcellular localization of proline-rich tyrosine kinase 2 during oocyte fertilization and early-embryo development in mice
Proline-rich tyrosine kinase 2 (Pyk2), a non-receptor tyrosine kinase, is a member of the focal adhesion kinase family and is highly expressed in oocytes. Using a combination of confocal microscopy and RNAi, we localized and studied the function of both Pyk2 and tyrosine-phosphorylated Pyk2 (p-Pyk2)...
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Published in: | Journal of Reproduction and Development 2016, Vol.62(4), pp.351-358 |
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creator | MENG, Xiao-qian DAI, Yuan-yuan JING, Lai-dong BAI, Jing LIU, Shu-zhen ZHENG, Ke-gang PAN, Jie |
description | Proline-rich tyrosine kinase 2 (Pyk2), a non-receptor tyrosine kinase, is a member of the focal adhesion kinase family and is highly expressed in oocytes. Using a combination of confocal microscopy and RNAi, we localized and studied the function of both Pyk2 and tyrosine-phosphorylated Pyk2 (p-Pyk2) during mouse oocyte fertilization and early embryo development. At the onset of fertilization, Pyk2 and p-Pyk2 were detected predominantly in sperm heads and the oocyte cytoplasm. Upon formation of male and female pronuclei, Pyk2 and its activated form leave the cytoplasm and accumulate in the two pronuclei. We detected Pyk2 in blastomere nuclei and found both Pyk2 and p-Pyk2 in the pre-blastula cytoplasm. Pyk2 and its activated form then disappeared from the blastula nuclei and localized to the perinuclear regions, where blastula cells come into contact with each other. Pyk2 knockdown via microinjection of siRNA into the zygote did not inhibit early embryo development. Our results suggest that Pyk2 plays multiple functional roles in mouse oocyte fertilization as well as throughout early embryo development. |
doi_str_mv | 10.1262/jrd.2016-015 |
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Reprod. Dev.</addtitle><description>Proline-rich tyrosine kinase 2 (Pyk2), a non-receptor tyrosine kinase, is a member of the focal adhesion kinase family and is highly expressed in oocytes. Using a combination of confocal microscopy and RNAi, we localized and studied the function of both Pyk2 and tyrosine-phosphorylated Pyk2 (p-Pyk2) during mouse oocyte fertilization and early embryo development. At the onset of fertilization, Pyk2 and p-Pyk2 were detected predominantly in sperm heads and the oocyte cytoplasm. Upon formation of male and female pronuclei, Pyk2 and its activated form leave the cytoplasm and accumulate in the two pronuclei. We detected Pyk2 in blastomere nuclei and found both Pyk2 and p-Pyk2 in the pre-blastula cytoplasm. Pyk2 and its activated form then disappeared from the blastula nuclei and localized to the perinuclear regions, where blastula cells come into contact with each other. Pyk2 knockdown via microinjection of siRNA into the zygote did not inhibit early embryo development. Our results suggest that Pyk2 plays multiple functional roles in mouse oocyte fertilization as well as throughout early embryo development.</description><subject>Early-embryo development</subject><subject>Fertilization</subject><subject>Mouse oocyte</subject><subject>Original</subject><subject>Pyk2</subject><issn>0916-8818</issn><issn>1348-4400</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2016</creationdate><recordtype>article</recordtype><recordid>eNpVkcFvFCEUxonR2LV68w_g6MGpwDAMczHRprVNmniongkwb3ZZGVhhpsm0_7yMWzfxAoH3ex-870PoPSUXlAn2aZ_6C0aoqAhtXqANrbmsOCfkJdqQrlxLSeUZepPznpCaNYK_RmesJVII0m3Q0_1sLHg_e52wj1Z796gnFwOOAz6k6F2AKjm7w9OSYi4n_MsFnQEz3M_JhS2O0S4T4AHS5E7dOvQYdPJLBaNJS8Q9PICPhxHChF3Ao7PwFr0atM_w7nk_Rz-vr35c3lR337_dXn65q6xoyFSZthdWA2Pa0IESydnQNrWRbKgtrxnRXa_XuYShXNjeSgnMCCPL8LbvbFOfo89H3cNsRuht-ULSXh2SG3VaVNRO_V8Jbqe28UE1hPC2I0Xgw7NAir9nyJMaXV5d0wHinBWVdVsz0TFe0I9H1Ba3coLh9Awlas1LlbzUmpcqeRX86xHf50lv4QTr4qX18BcuPXxd_jWdinank4JQ_wEMuqL6</recordid><startdate>2016</startdate><enddate>2016</enddate><creator>MENG, Xiao-qian</creator><creator>DAI, Yuan-yuan</creator><creator>JING, Lai-dong</creator><creator>BAI, Jing</creator><creator>LIU, Shu-zhen</creator><creator>ZHENG, Ke-gang</creator><creator>PAN, Jie</creator><general>THE SOCIETY FOR REPRODUCTION AND DEVELOPMENT</general><general>The Society for Reproduction and Development</general><scope>AAYXX</scope><scope>CITATION</scope><scope>7T5</scope><scope>H94</scope><scope>5PM</scope></search><sort><creationdate>2016</creationdate><title>Subcellular localization of proline-rich tyrosine kinase 2 during oocyte fertilization and early-embryo development in mice</title><author>MENG, Xiao-qian ; DAI, Yuan-yuan ; JING, Lai-dong ; BAI, Jing ; LIU, Shu-zhen ; ZHENG, Ke-gang ; PAN, Jie</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c650t-b7d6cae22ab1f10842f753b82f3c4320a9da25646b146cdc88e2b6b8916cd9c53</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2016</creationdate><topic>Early-embryo development</topic><topic>Fertilization</topic><topic>Mouse oocyte</topic><topic>Original</topic><topic>Pyk2</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>MENG, Xiao-qian</creatorcontrib><creatorcontrib>DAI, Yuan-yuan</creatorcontrib><creatorcontrib>JING, Lai-dong</creatorcontrib><creatorcontrib>BAI, Jing</creatorcontrib><creatorcontrib>LIU, Shu-zhen</creatorcontrib><creatorcontrib>ZHENG, Ke-gang</creatorcontrib><creatorcontrib>PAN, Jie</creatorcontrib><collection>CrossRef</collection><collection>Immunology Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Journal of Reproduction and Development</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>MENG, Xiao-qian</au><au>DAI, Yuan-yuan</au><au>JING, Lai-dong</au><au>BAI, Jing</au><au>LIU, Shu-zhen</au><au>ZHENG, Ke-gang</au><au>PAN, Jie</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Subcellular localization of proline-rich tyrosine kinase 2 during oocyte fertilization and early-embryo development in mice</atitle><jtitle>Journal of Reproduction and Development</jtitle><addtitle>J. Reprod. Dev.</addtitle><date>2016</date><risdate>2016</risdate><volume>62</volume><issue>4</issue><spage>351</spage><epage>358</epage><pages>351-358</pages><issn>0916-8818</issn><eissn>1348-4400</eissn><notes>ObjectType-Article-1</notes><notes>SourceType-Scholarly Journals-1</notes><notes>ObjectType-Feature-2</notes><notes>content type line 23</notes><abstract>Proline-rich tyrosine kinase 2 (Pyk2), a non-receptor tyrosine kinase, is a member of the focal adhesion kinase family and is highly expressed in oocytes. Using a combination of confocal microscopy and RNAi, we localized and studied the function of both Pyk2 and tyrosine-phosphorylated Pyk2 (p-Pyk2) during mouse oocyte fertilization and early embryo development. At the onset of fertilization, Pyk2 and p-Pyk2 were detected predominantly in sperm heads and the oocyte cytoplasm. Upon formation of male and female pronuclei, Pyk2 and its activated form leave the cytoplasm and accumulate in the two pronuclei. We detected Pyk2 in blastomere nuclei and found both Pyk2 and p-Pyk2 in the pre-blastula cytoplasm. Pyk2 and its activated form then disappeared from the blastula nuclei and localized to the perinuclear regions, where blastula cells come into contact with each other. Pyk2 knockdown via microinjection of siRNA into the zygote did not inhibit early embryo development. Our results suggest that Pyk2 plays multiple functional roles in mouse oocyte fertilization as well as throughout early embryo development.</abstract><pub>THE SOCIETY FOR REPRODUCTION AND DEVELOPMENT</pub><pmid>27086609</pmid><doi>10.1262/jrd.2016-015</doi><tpages>8</tpages><oa>free_for_read</oa></addata></record> |
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subjects | Early-embryo development Fertilization Mouse oocyte Original Pyk2 |
title | Subcellular localization of proline-rich tyrosine kinase 2 during oocyte fertilization and early-embryo development in mice |
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