Proteomic comparison reveals the contribution of chloroplast to salt tolerance of a wheat introgression line

We previously bred a salt tolerant wheat cv. SR3 with bread wheat cv. JN177 as the parent via asymmetric somatic hybridization, and found that the tolerance is partially attributed to the superior photosynthesis capacity. Here, we compared the proteomes of two cultivars to unravel the basis of super...

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Bibliographic Details
Published in:Scientific reports 2016-08, Vol.6 (1), p.32384-32384, Article 32384
Main Authors: Xu, Wenjing, Lv, Hongjun, Zhao, Mingming, Li, Yongchao, Qi, Yueying, Peng, Zhenying, Xia, Guangmin, Wang, Mengcheng
Format: Article
Language:English
Subjects:
Arabidopsis - genetics
Arabidopsis - growth & development
Chloroplasts
Chloroplasts - genetics
Gel electrophoresis
Gene Expression Regulation, Plant
Hybridization
Linolenic acid
Localization
Metabolism
Photosynthesis
Photosynthesis - genetics
Plant Proteins - genetics
Plants, Genetically Modified - genetics
Plants, Genetically Modified - growth & development
Protein biosynthesis
Protein turnover
Proteomics
Salinity tolerance
Salt-Tolerant Plants - genetics
Salt-Tolerant Plants - growth & development
Seedlings - genetics
Seedlings - growth & development
Stress, Physiological - genetics
Systems stability
Triticum - genetics
Triticum - growth & development
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Summary:We previously bred a salt tolerant wheat cv. SR3 with bread wheat cv. JN177 as the parent via asymmetric somatic hybridization, and found that the tolerance is partially attributed to the superior photosynthesis capacity. Here, we compared the proteomes of two cultivars to unravel the basis of superior photosynthesis capacity. In the maps of two dimensional difference gel electrophoresis (2D-DIGE), there were 26 differentially expressed proteins (DEPs), including 18 cultivar-based and 8 stress-responsive ones. 21 of 26 DEPs were identified and classified into four categories, including photosynthesis, photosynthesis system stability, linolenic acid metabolism, and protein synthesis in chloroplast. The chloroplast localization of some DEPs confirmed that the identified DEPs function in the chloroplast. The overexpression of a DEP enhanced salt tolerance in Arabidopsis thaliana. In line with these data, it is concluded that the contribution of chloroplast to high salinity tolerance of wheat cv. SR3 appears to include higher photosynthesis efficiency by promoting system protection and ROS clearance, stronger production of phytohormone JA by enhancing metabolism activity, and modulating the in chloroplast synthesis of proteins.
ISSN:2045-2322
2045-2322
DOI:10.1038/srep32384