In vivo effects of human adipose-derived stem cells reseeding on acellular bovine pericardium in nude mice

Tissue-engineered biologic products may be a viable option in the reconstruction of pelvic organ prolapse (POP). This study was based on the hypothesis that human adipose-derived stem cells (hASCs) are viable in acellular bovine pericardium (ABP), when reseeded by two different techniques, and thus,...

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Published in:Experimental biology and medicine (Maywood, N.J.) N.J.), 2016-01, Vol.241 (1), p.31-39
Main Authors: Wu, Qingkai, Dai, Miao, Xu, Peirong, Hou, Min, Teng, Yincheng, Feng, Jie
Format: Article
Language:English
Subjects:
Adipose Tissue - cytology
Adult
Animals
Cattle
Cell Differentiation - physiology
Female
Histocytochemistry
Humans
Luminescent Measurements
Mice, Nude
Optical Imaging
Original Research
Pericardium - cytology
Stem Cells - physiology
Tissue Engineering - methods
Tissue Transplantation - methods
Treatment Outcome
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cited_by cdi_FETCH-LOGICAL-c467t-28c8a3e6b9e9c408cfed6a4e95b069a748795a0c6fa4e7d598d6bde6a9d73c493
cites cdi_FETCH-LOGICAL-c467t-28c8a3e6b9e9c408cfed6a4e95b069a748795a0c6fa4e7d598d6bde6a9d73c493
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container_title Experimental biology and medicine (Maywood, N.J.)
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creator Wu, Qingkai
Dai, Miao
Xu, Peirong
Hou, Min
Teng, Yincheng
Feng, Jie
description Tissue-engineered biologic products may be a viable option in the reconstruction of pelvic organ prolapse (POP). This study was based on the hypothesis that human adipose-derived stem cells (hASCs) are viable in acellular bovine pericardium (ABP), when reseeded by two different techniques, and thus, aid in the reconstruction. To investigate the reseeding of hASCs on ABP grafts by using non-invasive bioluminescence imaging (BLI), and to identify the effective hASCs–scaffold combinations that enabled regeneration. Thirty female athymic nude mice were randomly divided into three groups: In the VIVO group, ABPs were implanted in the subcutaneous pockets and enhanced green fluorescent protein luciferase (eGFP·Luc)-hASCs (1 × 106 cells/50 µL) were injected on the ABP at the same time. In the VITRO group, the mice were implanted with grafts that ABP were co-cultured with eGFP·Luc-hASCs in vitro. The BLANK group mice were implanted with ABP only. The eGFP·Luc-hASCs reseeded on ABP were analyzed by BLI, histology, and immunohistochemistry. The eGFP·Luc-hASCs reseeded on ABP could be visualized at 12 weeks in vivo. Histology revealed that the VIVO group displayed the highest cell ingrowths, small vessels, and percent of collagen content per unit area. Desmin and α-smooth muscle actin were positive at the same site in the VIVO group cells. However, few smooth muscles were observed in the VITRO and BLANK groups. These results suggest that hASCs reseeded on ABP in vivo during surgery may further enhance the properties of ABP and may promote regeneration at the recipient site, resulting in a promising treatment option for POP.
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This study was based on the hypothesis that human adipose-derived stem cells (hASCs) are viable in acellular bovine pericardium (ABP), when reseeded by two different techniques, and thus, aid in the reconstruction. To investigate the reseeding of hASCs on ABP grafts by using non-invasive bioluminescence imaging (BLI), and to identify the effective hASCs–scaffold combinations that enabled regeneration. Thirty female athymic nude mice were randomly divided into three groups: In the VIVO group, ABPs were implanted in the subcutaneous pockets and enhanced green fluorescent protein luciferase (eGFP·Luc)-hASCs (1 × 106 cells/50 µL) were injected on the ABP at the same time. In the VITRO group, the mice were implanted with grafts that ABP were co-cultured with eGFP·Luc-hASCs in vitro. The BLANK group mice were implanted with ABP only. The eGFP·Luc-hASCs reseeded on ABP were analyzed by BLI, histology, and immunohistochemistry. The eGFP·Luc-hASCs reseeded on ABP could be visualized at 12 weeks in vivo. Histology revealed that the VIVO group displayed the highest cell ingrowths, small vessels, and percent of collagen content per unit area. Desmin and α-smooth muscle actin were positive at the same site in the VIVO group cells. However, few smooth muscles were observed in the VITRO and BLANK groups. 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This study was based on the hypothesis that human adipose-derived stem cells (hASCs) are viable in acellular bovine pericardium (ABP), when reseeded by two different techniques, and thus, aid in the reconstruction. To investigate the reseeding of hASCs on ABP grafts by using non-invasive bioluminescence imaging (BLI), and to identify the effective hASCs–scaffold combinations that enabled regeneration. Thirty female athymic nude mice were randomly divided into three groups: In the VIVO group, ABPs were implanted in the subcutaneous pockets and enhanced green fluorescent protein luciferase (eGFP·Luc)-hASCs (1 × 106 cells/50 µL) were injected on the ABP at the same time. In the VITRO group, the mice were implanted with grafts that ABP were co-cultured with eGFP·Luc-hASCs in vitro. The BLANK group mice were implanted with ABP only. The eGFP·Luc-hASCs reseeded on ABP were analyzed by BLI, histology, and immunohistochemistry. The eGFP·Luc-hASCs reseeded on ABP could be visualized at 12 weeks in vivo. Histology revealed that the VIVO group displayed the highest cell ingrowths, small vessels, and percent of collagen content per unit area. Desmin and α-smooth muscle actin were positive at the same site in the VIVO group cells. However, few smooth muscles were observed in the VITRO and BLANK groups. These results suggest that hASCs reseeded on ABP in vivo during surgery may further enhance the properties of ABP and may promote regeneration at the recipient site, resulting in a promising treatment option for POP.</abstract><cop>London, England</cop><pub>SAGE Publications</pub><pmid>26253192</pmid><doi>10.1177/1535370215597193</doi><tpages>9</tpages><oa>free_for_read</oa></addata></record>
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subjects Adipose Tissue - cytology
Adult
Animals
Cattle
Cell Differentiation - physiology
Female
Histocytochemistry
Humans
Luminescent Measurements
Mice, Nude
Optical Imaging
Original Research
Pericardium - cytology
Stem Cells - physiology
Tissue Engineering - methods
Tissue Transplantation - methods
Treatment Outcome
title In vivo effects of human adipose-derived stem cells reseeding on acellular bovine pericardium in nude mice
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