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pathogenesis of H3N8 canine influenza virus in chickens, turkeys and ducks
Background: Canine influenza virus (CIV) of the H3N8 subtype has emerged in dog populations throughout the United States where it has become endemic in kennels and animal shelters in some regions. It has not previously been determined whether thecanine-adapted H3N8 influenza virus can be transmitted...
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| Published in: | Influenza and other respiratory viruses 2010-11, Vol.4 (6), p.353-356 |
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| container_title | Influenza and other respiratory viruses |
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| creator | McKinley, Enid T Spackman, Erica Pantin-Jackwood, Mary J |
| description | Background: Canine influenza virus (CIV) of the H3N8 subtype has emerged in dog populations throughout the United States where it has become endemic in kennels and animal shelters in some regions. It has not previously been determined whether thecanine-adapted H3N8 influenza virus can be transmitted to chickens, turkeys or ducks which are economically important animals that are susceptible to type A influenza virus from numerous species. Methods: Four-week-old specific pathogen–free (SPF) chickens, 3-week-old SPF turkeys and 2-week-old commercial Pekin ducks were inoculated with 10^5·^2 50% tissue culture infectious doses of CIV per bird by the intra-choanal route. The birds were observed daily, and at 2 and 4 days post-inoculation (DPI), two inoculated birds and one sham-inoculated control bird were euthanized and necropsied to evaluate gross lesions and to collect tissues for microscopic examination. Cloacal and oral swabs were collected at 2, 4 and 7 DPI to evaluate virus shed by real-time RT-PCR (rRTPCR). Two weeks post-infection, sera were collected from all remaining birds for type A influenza antibody detection by hemagglutination inhibition assay. Results: Clinical signs and gross lesions were not observed in any of the birds of any species nor did any seroconvert. Oral and Cloacal swab material was negative for virus by rRT-PCR. Conclusions Chickens, turkeys and Pekin ducks are not susceptible to infection with CIV by simulated respiratory exposure route with the dose administered. |
| doi_str_mv | 10.1111/j.1750-2659.2010.00165.x |
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| fullrecord | <record><control><sourceid>proquest_24P</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_4634604</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>860392920</sourcerecordid><originalsourceid>FETCH-LOGICAL-c5565-5793139cf4f770e316c3a77c1857e3349caeb2f1060045e9ddbad1b54c4ef3a73</originalsourceid><addsrcrecordid>eNqNkc1uEzEUhS0EoqXwCmCJBRsS_O-xhJBQBbSoAgkoW8vx3EmcTOxgZ0rD0-MhJSpswBtf-37nyL4HIUzJlNb1YjmlWpIJU9JMGam3hFAlp9d30PGhcfdWfYQelLIkRKpGivvoiBEjG8PMMXq_cdtFmkOEEgpOHT7jHxrsXQwRcIhdP0D84fBVyEOpZ-wXwa8glud4O-QV7Ap2scXt4FflIbrXub7Ao5v9BF2-ffPl9Gxy8fHd-enri4mXUsmJ1IZTbnwnOq0JcKo8d1p72kgNnAvjHcxYR4kiREgwbTtzLZ1J4QV0leQn6NXedzPM1tB6iNvservJYe3yziYX7J-dGBZ2nq6sUFwoIqrBsxuDnL4NULZ2HYqHvncR0lBsowivw2Hkn6SWRjLO6Oj59C9ymYYc6xwsE4QxTRljlWr2lM-plAzd4dWU2DFZu7RjaHYMzY7J2l_J2usqfXz71wfh7ygr8HIPfA897P7b2J5_-lqLKn-yl3cuWTfPodjLzxWUldOUN5z_BJiUuhU</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2402271222</pqid></control><display><type>article</type><title>pathogenesis of H3N8 canine influenza virus in chickens, turkeys and ducks</title><source>Open Access: Wiley-Blackwell Open Access Journals</source><creator>McKinley, Enid T ; Spackman, Erica ; Pantin-Jackwood, Mary J</creator><creatorcontrib>McKinley, Enid T ; Spackman, Erica ; Pantin-Jackwood, Mary J</creatorcontrib><description>Background: Canine influenza virus (CIV) of the H3N8 subtype has emerged in dog populations throughout the United States where it has become endemic in kennels and animal shelters in some regions. It has not previously been determined whether thecanine-adapted H3N8 influenza virus can be transmitted to chickens, turkeys or ducks which are economically important animals that are susceptible to type A influenza virus from numerous species. Methods: Four-week-old specific pathogen–free (SPF) chickens, 3-week-old SPF turkeys and 2-week-old commercial Pekin ducks were inoculated with 10^5·^2 50% tissue culture infectious doses of CIV per bird by the intra-choanal route. The birds were observed daily, and at 2 and 4 days post-inoculation (DPI), two inoculated birds and one sham-inoculated control bird were euthanized and necropsied to evaluate gross lesions and to collect tissues for microscopic examination. Cloacal and oral swabs were collected at 2, 4 and 7 DPI to evaluate virus shed by real-time RT-PCR (rRTPCR). Two weeks post-infection, sera were collected from all remaining birds for type A influenza antibody detection by hemagglutination inhibition assay. Results: Clinical signs and gross lesions were not observed in any of the birds of any species nor did any seroconvert. Oral and Cloacal swab material was negative for virus by rRT-PCR. Conclusions Chickens, turkeys and Pekin ducks are not susceptible to infection with CIV by simulated respiratory exposure route with the dose administered.</description><identifier>ISSN: 1750-2659</identifier><identifier>ISSN: 1750-2640</identifier><identifier>EISSN: 1750-2659</identifier><identifier>DOI: 10.1111/j.1750-2659.2010.00165.x</identifier><identifier>PMID: 20958929</identifier><language>eng</language><publisher>Oxford, UK: Blackwell Publishing Ltd</publisher><subject>administered dose ; Animal euthanasia ; Animals ; Antibodies ; Antibodies, Viral - blood ; antibody detection ; Aquatic birds ; biological resistance ; Birds ; Canine influenza ; Chickens ; Cloaca - virology ; Disease Models, Animal ; Dog Diseases - transmission ; Dog Diseases - virology ; Dogs ; Ducks ; Evaluation ; Hemagglutination inhibition ; hemagglutination inhibition test ; Hemagglutination Inhibition Tests ; Infection ; Infections ; Influenza ; Influenza A virus ; Influenza A Virus, H3N8 Subtype - isolation & purification ; Influenza A Virus, H3N8 Subtype - pathogenicity ; Influenza in Birds - pathology ; Influenza in Birds - virology ; Influenza virus ; inhalation exposure ; Inoculation ; Lesions ; Mammals ; microscopy ; Mortality ; Mouth - virology ; Original ; Pathogenesis ; Pekin ; Polymerase chain reaction ; Poultry ; Poultry Diseases - pathology ; Poultry Diseases - virology ; Respiratory diseases ; Reverse Transcriptase Polymerase Chain Reaction ; RNA, Viral - genetics ; RNA, Viral - isolation & purification ; Shelter ; specific pathogen-free animals ; Tissue culture ; tissues ; Turkeys ; United States ; viral shedding ; Virus Shedding ; Viruses ; Waterfowl</subject><ispartof>Influenza and other respiratory viruses, 2010-11, Vol.4 (6), p.353-356</ispartof><rights>2010 Blackwell Publishing Ltd</rights><rights>2010 Blackwell Publishing Ltd.</rights><rights>Copyright John Wiley & Sons, Inc. Nov 2010</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c5565-5793139cf4f770e316c3a77c1857e3349caeb2f1060045e9ddbad1b54c4ef3a73</citedby><cites>FETCH-LOGICAL-c5565-5793139cf4f770e316c3a77c1857e3349caeb2f1060045e9ddbad1b54c4ef3a73</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4634604/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4634604/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,315,733,786,790,891,11589,27957,27958,46087,46511,53827,53829</link.rule.ids><linktorsrc>$$Uhttps://onlinelibrary.wiley.com/doi/abs/10.1111%2Fj.1750-2659.2010.00165.x$$EView_record_in_Wiley-Blackwell$$FView_record_in_$$GWiley-Blackwell</linktorsrc><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/20958929$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>McKinley, Enid T</creatorcontrib><creatorcontrib>Spackman, Erica</creatorcontrib><creatorcontrib>Pantin-Jackwood, Mary J</creatorcontrib><title>pathogenesis of H3N8 canine influenza virus in chickens, turkeys and ducks</title><title>Influenza and other respiratory viruses</title><addtitle>Influenza Other Respir Viruses</addtitle><description>Background: Canine influenza virus (CIV) of the H3N8 subtype has emerged in dog populations throughout the United States where it has become endemic in kennels and animal shelters in some regions. It has not previously been determined whether thecanine-adapted H3N8 influenza virus can be transmitted to chickens, turkeys or ducks which are economically important animals that are susceptible to type A influenza virus from numerous species. Methods: Four-week-old specific pathogen–free (SPF) chickens, 3-week-old SPF turkeys and 2-week-old commercial Pekin ducks were inoculated with 10^5·^2 50% tissue culture infectious doses of CIV per bird by the intra-choanal route. The birds were observed daily, and at 2 and 4 days post-inoculation (DPI), two inoculated birds and one sham-inoculated control bird were euthanized and necropsied to evaluate gross lesions and to collect tissues for microscopic examination. Cloacal and oral swabs were collected at 2, 4 and 7 DPI to evaluate virus shed by real-time RT-PCR (rRTPCR). Two weeks post-infection, sera were collected from all remaining birds for type A influenza antibody detection by hemagglutination inhibition assay. Results: Clinical signs and gross lesions were not observed in any of the birds of any species nor did any seroconvert. Oral and Cloacal swab material was negative for virus by rRT-PCR. Conclusions Chickens, turkeys and Pekin ducks are not susceptible to infection with CIV by simulated respiratory exposure route with the dose administered.</description><subject>administered dose</subject><subject>Animal euthanasia</subject><subject>Animals</subject><subject>Antibodies</subject><subject>Antibodies, Viral - blood</subject><subject>antibody detection</subject><subject>Aquatic birds</subject><subject>biological resistance</subject><subject>Birds</subject><subject>Canine influenza</subject><subject>Chickens</subject><subject>Cloaca - virology</subject><subject>Disease Models, Animal</subject><subject>Dog Diseases - transmission</subject><subject>Dog Diseases - virology</subject><subject>Dogs</subject><subject>Ducks</subject><subject>Evaluation</subject><subject>Hemagglutination inhibition</subject><subject>hemagglutination inhibition test</subject><subject>Hemagglutination Inhibition Tests</subject><subject>Infection</subject><subject>Infections</subject><subject>Influenza</subject><subject>Influenza A virus</subject><subject>Influenza A Virus, H3N8 Subtype - isolation & purification</subject><subject>Influenza A Virus, H3N8 Subtype - pathogenicity</subject><subject>Influenza in Birds - pathology</subject><subject>Influenza in Birds - virology</subject><subject>Influenza virus</subject><subject>inhalation exposure</subject><subject>Inoculation</subject><subject>Lesions</subject><subject>Mammals</subject><subject>microscopy</subject><subject>Mortality</subject><subject>Mouth - virology</subject><subject>Original</subject><subject>Pathogenesis</subject><subject>Pekin</subject><subject>Polymerase chain reaction</subject><subject>Poultry</subject><subject>Poultry Diseases - pathology</subject><subject>Poultry Diseases - virology</subject><subject>Respiratory diseases</subject><subject>Reverse Transcriptase Polymerase Chain Reaction</subject><subject>RNA, Viral - genetics</subject><subject>RNA, Viral - isolation & purification</subject><subject>Shelter</subject><subject>specific pathogen-free animals</subject><subject>Tissue culture</subject><subject>tissues</subject><subject>Turkeys</subject><subject>United States</subject><subject>viral shedding</subject><subject>Virus Shedding</subject><subject>Viruses</subject><subject>Waterfowl</subject><issn>1750-2659</issn><issn>1750-2640</issn><issn>1750-2659</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2010</creationdate><recordtype>article</recordtype><recordid>eNqNkc1uEzEUhS0EoqXwCmCJBRsS_O-xhJBQBbSoAgkoW8vx3EmcTOxgZ0rD0-MhJSpswBtf-37nyL4HIUzJlNb1YjmlWpIJU9JMGam3hFAlp9d30PGhcfdWfYQelLIkRKpGivvoiBEjG8PMMXq_cdtFmkOEEgpOHT7jHxrsXQwRcIhdP0D84fBVyEOpZ-wXwa8glud4O-QV7Ap2scXt4FflIbrXub7Ao5v9BF2-ffPl9Gxy8fHd-enri4mXUsmJ1IZTbnwnOq0JcKo8d1p72kgNnAvjHcxYR4kiREgwbTtzLZ1J4QV0leQn6NXedzPM1tB6iNvservJYe3yziYX7J-dGBZ2nq6sUFwoIqrBsxuDnL4NULZ2HYqHvncR0lBsowivw2Hkn6SWRjLO6Oj59C9ymYYc6xwsE4QxTRljlWr2lM-plAzd4dWU2DFZu7RjaHYMzY7J2l_J2usqfXz71wfh7ygr8HIPfA897P7b2J5_-lqLKn-yl3cuWTfPodjLzxWUldOUN5z_BJiUuhU</recordid><startdate>201011</startdate><enddate>201011</enddate><creator>McKinley, Enid T</creator><creator>Spackman, Erica</creator><creator>Pantin-Jackwood, Mary J</creator><general>Blackwell Publishing Ltd</general><general>John Wiley & Sons, Inc</general><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7T2</scope><scope>7U9</scope><scope>C1K</scope><scope>H94</scope><scope>K9.</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>201011</creationdate><title>pathogenesis of H3N8 canine influenza virus in chickens, turkeys and ducks</title><author>McKinley, Enid T ; Spackman, Erica ; Pantin-Jackwood, Mary J</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c5565-5793139cf4f770e316c3a77c1857e3349caeb2f1060045e9ddbad1b54c4ef3a73</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2010</creationdate><topic>administered dose</topic><topic>Animal euthanasia</topic><topic>Animals</topic><topic>Antibodies</topic><topic>Antibodies, Viral - blood</topic><topic>antibody detection</topic><topic>Aquatic birds</topic><topic>biological resistance</topic><topic>Birds</topic><topic>Canine influenza</topic><topic>Chickens</topic><topic>Cloaca - virology</topic><topic>Disease Models, Animal</topic><topic>Dog Diseases - transmission</topic><topic>Dog Diseases - virology</topic><topic>Dogs</topic><topic>Ducks</topic><topic>Evaluation</topic><topic>Hemagglutination inhibition</topic><topic>hemagglutination inhibition test</topic><topic>Hemagglutination Inhibition Tests</topic><topic>Infection</topic><topic>Infections</topic><topic>Influenza</topic><topic>Influenza A virus</topic><topic>Influenza A Virus, H3N8 Subtype - isolation & purification</topic><topic>Influenza A Virus, H3N8 Subtype - pathogenicity</topic><topic>Influenza in Birds - pathology</topic><topic>Influenza in Birds - virology</topic><topic>Influenza virus</topic><topic>inhalation exposure</topic><topic>Inoculation</topic><topic>Lesions</topic><topic>Mammals</topic><topic>microscopy</topic><topic>Mortality</topic><topic>Mouth - virology</topic><topic>Original</topic><topic>Pathogenesis</topic><topic>Pekin</topic><topic>Polymerase chain reaction</topic><topic>Poultry</topic><topic>Poultry Diseases - pathology</topic><topic>Poultry Diseases - virology</topic><topic>Respiratory diseases</topic><topic>Reverse Transcriptase Polymerase Chain Reaction</topic><topic>RNA, Viral - genetics</topic><topic>RNA, Viral - isolation & purification</topic><topic>Shelter</topic><topic>specific pathogen-free animals</topic><topic>Tissue culture</topic><topic>tissues</topic><topic>Turkeys</topic><topic>United States</topic><topic>viral shedding</topic><topic>Virus Shedding</topic><topic>Viruses</topic><topic>Waterfowl</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>McKinley, Enid T</creatorcontrib><creatorcontrib>Spackman, Erica</creatorcontrib><creatorcontrib>Pantin-Jackwood, Mary J</creatorcontrib><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Health and Safety Science Abstracts (Full archive)</collection><collection>Virology and AIDS Abstracts</collection><collection>Environmental Sciences and Pollution Management</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Influenza and other respiratory viruses</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext_linktorsrc</fulltext></delivery><addata><au>McKinley, Enid T</au><au>Spackman, Erica</au><au>Pantin-Jackwood, Mary J</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>pathogenesis of H3N8 canine influenza virus in chickens, turkeys and ducks</atitle><jtitle>Influenza and other respiratory viruses</jtitle><addtitle>Influenza Other Respir Viruses</addtitle><date>2010-11</date><risdate>2010</risdate><volume>4</volume><issue>6</issue><spage>353</spage><epage>356</epage><pages>353-356</pages><issn>1750-2659</issn><issn>1750-2640</issn><eissn>1750-2659</eissn><notes>http://dx.doi.org/10.1111/j.1750-2659.2010.00165.x</notes><notes>http://handle.nal.usda.gov/10113/60755</notes><notes>ObjectType-Article-1</notes><notes>SourceType-Scholarly Journals-1</notes><notes>ObjectType-Feature-2</notes><notes>content type line 23</notes><notes>ObjectType-Article-2</notes><notes>ObjectType-Feature-1</notes><abstract>Background: Canine influenza virus (CIV) of the H3N8 subtype has emerged in dog populations throughout the United States where it has become endemic in kennels and animal shelters in some regions. It has not previously been determined whether thecanine-adapted H3N8 influenza virus can be transmitted to chickens, turkeys or ducks which are economically important animals that are susceptible to type A influenza virus from numerous species. Methods: Four-week-old specific pathogen–free (SPF) chickens, 3-week-old SPF turkeys and 2-week-old commercial Pekin ducks were inoculated with 10^5·^2 50% tissue culture infectious doses of CIV per bird by the intra-choanal route. The birds were observed daily, and at 2 and 4 days post-inoculation (DPI), two inoculated birds and one sham-inoculated control bird were euthanized and necropsied to evaluate gross lesions and to collect tissues for microscopic examination. Cloacal and oral swabs were collected at 2, 4 and 7 DPI to evaluate virus shed by real-time RT-PCR (rRTPCR). Two weeks post-infection, sera were collected from all remaining birds for type A influenza antibody detection by hemagglutination inhibition assay. Results: Clinical signs and gross lesions were not observed in any of the birds of any species nor did any seroconvert. Oral and Cloacal swab material was negative for virus by rRT-PCR. Conclusions Chickens, turkeys and Pekin ducks are not susceptible to infection with CIV by simulated respiratory exposure route with the dose administered.</abstract><cop>Oxford, UK</cop><pub>Blackwell Publishing Ltd</pub><pmid>20958929</pmid><doi>10.1111/j.1750-2659.2010.00165.x</doi><tpages>4</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | administered dose Animal euthanasia Animals Antibodies Antibodies, Viral - blood antibody detection Aquatic birds biological resistance Birds Canine influenza Chickens Cloaca - virology Disease Models, Animal Dog Diseases - transmission Dog Diseases - virology Dogs Ducks Evaluation Hemagglutination inhibition hemagglutination inhibition test Hemagglutination Inhibition Tests Infection Infections Influenza Influenza A virus Influenza A Virus, H3N8 Subtype - isolation & purification Influenza A Virus, H3N8 Subtype - pathogenicity Influenza in Birds - pathology Influenza in Birds - virology Influenza virus inhalation exposure Inoculation Lesions Mammals microscopy Mortality Mouth - virology Original Pathogenesis Pekin Polymerase chain reaction Poultry Poultry Diseases - pathology Poultry Diseases - virology Respiratory diseases Reverse Transcriptase Polymerase Chain Reaction RNA, Viral - genetics RNA, Viral - isolation & purification Shelter specific pathogen-free animals Tissue culture tissues Turkeys United States viral shedding Virus Shedding Viruses Waterfowl |
| title | pathogenesis of H3N8 canine influenza virus in chickens, turkeys and ducks |
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