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The molecular mechanism of Zinc acquisition by the neisserial outer-membrane transporter ZnuD
Invading bacteria from the Neisseriaceae, Acinetobacteriaceae, Bordetellaceae and Moraxellaceae families express the conserved outer-membrane zinc transporter zinc-uptake component D (ZnuD) to overcome nutritional restriction imposed by the host organism during infection. Here we demonstrate that Zn...
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Published in: | Nature communications 2015-08, Vol.6 (1), p.7996-7996, Article 7996 |
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creator | Calmettes, Charles Ing, Christopher Buckwalter, Carolyn M El Bakkouri, Majida Chieh-Lin Lai, Christine Pogoutse, Anastassia Gray-Owen, Scott D Pomès, Régis Moraes, Trevor F |
description | Invading bacteria from the Neisseriaceae, Acinetobacteriaceae, Bordetellaceae and Moraxellaceae families express the conserved outer-membrane zinc transporter zinc-uptake component D (ZnuD) to overcome nutritional restriction imposed by the host organism during infection. Here we demonstrate that ZnuD is required for efficient systemic infections by the causative agent of bacterial meningitis, Neisseria meningitidis, in a mouse model. We also combine X-ray crystallography and molecular dynamics simulations to gain insight into the mechanism of zinc recognition and transport across the bacterial outer-membrane by ZnuD. Because ZnuD is also considered a promising vaccine candidate against N. meningitidis, we use several ZnuD structural intermediates to map potential antigenic epitopes, and propose a mechanism by which ZnuD can maintain high sequence conservation yet avoid immune recognition by altering the conformation of surface-exposed loops. |
doi_str_mv | 10.1038/ncomms8996 |
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Here we demonstrate that ZnuD is required for efficient systemic infections by the causative agent of bacterial meningitis, Neisseria meningitidis, in a mouse model. We also combine X-ray crystallography and molecular dynamics simulations to gain insight into the mechanism of zinc recognition and transport across the bacterial outer-membrane by ZnuD. Because ZnuD is also considered a promising vaccine candidate against N. meningitidis, we use several ZnuD structural intermediates to map potential antigenic epitopes, and propose a mechanism by which ZnuD can maintain high sequence conservation yet avoid immune recognition by altering the conformation of surface-exposed loops.</description><identifier>ISSN: 2041-1723</identifier><identifier>EISSN: 2041-1723</identifier><identifier>DOI: 10.1038/ncomms8996</identifier><identifier>PMID: 26282243</identifier><language>eng</language><publisher>England: Nature Publishing Group</publisher><subject>Animals ; Bacterial Outer Membrane Proteins - genetics ; Bacterial Outer Membrane Proteins - metabolism ; Bacterial Proteins - genetics ; Bacterial Proteins - metabolism ; Cation Transport Proteins - genetics ; Cation Transport Proteins - metabolism ; Female ; Gene Expression Regulation, Bacterial ; Male ; Mice ; Mice, Inbred C57BL ; Models, Molecular ; Neisseria meningitidis - metabolism ; Protein Conformation ; Sepsis - microbiology ; Zinc - metabolism</subject><ispartof>Nature communications, 2015-08, Vol.6 (1), p.7996-7996, Article 7996</ispartof><rights>Copyright Nature Publishing Group Aug 2015</rights><rights>Copyright © 2015, Nature Publishing Group, a division of Macmillan Publishers Limited. 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Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Nature communications</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Calmettes, Charles</au><au>Ing, Christopher</au><au>Buckwalter, Carolyn M</au><au>El Bakkouri, Majida</au><au>Chieh-Lin Lai, Christine</au><au>Pogoutse, Anastassia</au><au>Gray-Owen, Scott D</au><au>Pomès, Régis</au><au>Moraes, Trevor F</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The molecular mechanism of Zinc acquisition by the neisserial outer-membrane transporter ZnuD</atitle><jtitle>Nature communications</jtitle><addtitle>Nat Commun</addtitle><date>2015-08-18</date><risdate>2015</risdate><volume>6</volume><issue>1</issue><spage>7996</spage><epage>7996</epage><pages>7996-7996</pages><artnum>7996</artnum><issn>2041-1723</issn><eissn>2041-1723</eissn><notes>ObjectType-Article-1</notes><notes>SourceType-Scholarly Journals-1</notes><notes>ObjectType-Feature-2</notes><notes>content type line 23</notes><abstract>Invading bacteria from the Neisseriaceae, Acinetobacteriaceae, Bordetellaceae and Moraxellaceae families express the conserved outer-membrane zinc transporter zinc-uptake component D (ZnuD) to overcome nutritional restriction imposed by the host organism during infection. 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subjects | Animals Bacterial Outer Membrane Proteins - genetics Bacterial Outer Membrane Proteins - metabolism Bacterial Proteins - genetics Bacterial Proteins - metabolism Cation Transport Proteins - genetics Cation Transport Proteins - metabolism Female Gene Expression Regulation, Bacterial Male Mice Mice, Inbred C57BL Models, Molecular Neisseria meningitidis - metabolism Protein Conformation Sepsis - microbiology Zinc - metabolism |
title | The molecular mechanism of Zinc acquisition by the neisserial outer-membrane transporter ZnuD |
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