SOS2-LIKE PROTEIN KINASE5, an SNF1-RELATED PROTEIN KINASE3-Type Protein Kinase, Is Important for Abscisic Acid Responses in Arabidopsis through Phosphorylation of ABSCISIC ACID-INSENSITIVE51[OPEN]

A salt sensitive-like protein kinase and phosphorylation of its unique target affect ABA inhibition of seed germination. Abscisic acid ( ABA ) plays an essential role in seed germination. In this study, we demonstrate that one SNF1-RELATED PROTEIN KINASE3-type protein kinase, SOS2-LIKE PROTEIN KINAS...

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Published in:Plant physiology (Bethesda) 2015-04, Vol.168 (2), p.659-676
Main Authors: Zhou, Xiaona, Hao, Hongmei, Zhang, Yuguo, Bai, Yili, Zhu, Wenbo, Qin, Yunxia, Yuan, Feifei, Zhao, Feiyi, Wang, Mengyao, Hu, Jingjiang, Xu, Hong, Guo, Aiguang, Zhao, Huixian, Zhao, Yang, Cao, Cuiling, Yang, Yongqing, Schumaker, Karen S., Guo, Yan, Xie, Chang Gen
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container_issue 2
container_start_page 659
container_title Plant physiology (Bethesda)
container_volume 168
creator Zhou, Xiaona
Hao, Hongmei
Zhang, Yuguo
Bai, Yili
Zhu, Wenbo
Qin, Yunxia
Yuan, Feifei
Zhao, Feiyi
Wang, Mengyao
Hu, Jingjiang
Xu, Hong
Guo, Aiguang
Zhao, Huixian
Zhao, Yang
Cao, Cuiling
Yang, Yongqing
Schumaker, Karen S.
Guo, Yan
Xie, Chang Gen
description A salt sensitive-like protein kinase and phosphorylation of its unique target affect ABA inhibition of seed germination. Abscisic acid ( ABA ) plays an essential role in seed germination. In this study, we demonstrate that one SNF1-RELATED PROTEIN KINASE3-type protein kinase, SOS2-LIKE PROTEIN KINASE5 (PKS5), is involved in ABA signal transduction via the phosphorylation of an interacting protein, ABSCISIC ACID-INSENSITIVE5 (ABI5). We found that pks5-3 and pks5-4 , two previously identified PKS5 superactive kinase mutants with point mutations in the PKS5 FISL/NAF (a conserved peptide that is necessary for interaction with SOS3 or SOS3-LIKE CALCIUM BINDING PROTEINs) motif and the kinase domain, respectively, are hypersensitive to ABA during seed germination. PKS5 was found to interact with ABI5 in yeast ( Saccharomyces cerevisiae ), and this interaction was further confirmed in planta using bimolecular fluorescence complementation. Genetic studies revealed that ABI5 is epistatic to PKS5 . PKS5 phosphorylates a serine (Ser) residue at position 42 in ABI5 and regulates ABA -responsive gene expression. This phosphorylation was induced by ABA in vivo and transactivated ABI5. Expression of ABI5, in which Ser-42 was mutated to alanine, could not fully rescue the ABA -insensitive phenotypes of the abi5-8 and pks5-4abi5-8 mutants. In contrast, mutating Ser-42 to aspartate rescued the ABA insensitivity of these mutants. These data demonstrate that PKS5-mediated phosphorylation of ABI5 at Ser-42 is critical for the ABA regulation of seed germination and gene expression in Arabidopsis ( Arabidopsis thaliana ).
doi_str_mv 10.1104/pp.114.255455
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Abscisic acid ( ABA ) plays an essential role in seed germination. In this study, we demonstrate that one SNF1-RELATED PROTEIN KINASE3-type protein kinase, SOS2-LIKE PROTEIN KINASE5 (PKS5), is involved in ABA signal transduction via the phosphorylation of an interacting protein, ABSCISIC ACID-INSENSITIVE5 (ABI5). We found that pks5-3 and pks5-4 , two previously identified PKS5 superactive kinase mutants with point mutations in the PKS5 FISL/NAF (a conserved peptide that is necessary for interaction with SOS3 or SOS3-LIKE CALCIUM BINDING PROTEINs) motif and the kinase domain, respectively, are hypersensitive to ABA during seed germination. PKS5 was found to interact with ABI5 in yeast ( Saccharomyces cerevisiae ), and this interaction was further confirmed in planta using bimolecular fluorescence complementation. Genetic studies revealed that ABI5 is epistatic to PKS5 . PKS5 phosphorylates a serine (Ser) residue at position 42 in ABI5 and regulates ABA -responsive gene expression. 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X.Z., C.G.X., H.H., Y.Z., Y.B., W.Z., F.Y., and M.W. performed the research; X.Z., C.G.X., H.H., Y.Z., Y.B., W.Z., Y.Q., F.Z., J.H., H.X., A.G., H.Z., Y.Z., C.C., and Y.Y. contributed to data analysis; C.G.X., X.Z., Y.G., and K.S.S. wrote the article.</notes><notes>www.plantphysiol.org/cgi/doi/10.1104/pp.114.255455</notes><notes>These authors contributed equally to the article.</notes><notes>Present address: School of Life Science and Chemistry, Tianshui Normal University, Tianshui, Ganshu 741000, China.</notes><notes>Present address: Rubber Research Institute, Chinese Academy of Tropical Agricultural Sciences, Danzhou, Hainan 571737, China.</notes><notes>The author responsible for distribution of materials integral to the findings presented in this article in accordance with the policy described in the Instructions for Authors (www.plantphysiol.org) is: Chang Gen Xie (changen.xie@nwsuaf.edu.cn).</notes><abstract>A salt sensitive-like protein kinase and phosphorylation of its unique target affect ABA inhibition of seed germination. Abscisic acid ( ABA ) plays an essential role in seed germination. In this study, we demonstrate that one SNF1-RELATED PROTEIN KINASE3-type protein kinase, SOS2-LIKE PROTEIN KINASE5 (PKS5), is involved in ABA signal transduction via the phosphorylation of an interacting protein, ABSCISIC ACID-INSENSITIVE5 (ABI5). We found that pks5-3 and pks5-4 , two previously identified PKS5 superactive kinase mutants with point mutations in the PKS5 FISL/NAF (a conserved peptide that is necessary for interaction with SOS3 or SOS3-LIKE CALCIUM BINDING PROTEINs) motif and the kinase domain, respectively, are hypersensitive to ABA during seed germination. PKS5 was found to interact with ABI5 in yeast ( Saccharomyces cerevisiae ), and this interaction was further confirmed in planta using bimolecular fluorescence complementation. Genetic studies revealed that ABI5 is epistatic to PKS5 . PKS5 phosphorylates a serine (Ser) residue at position 42 in ABI5 and regulates ABA -responsive gene expression. This phosphorylation was induced by ABA in vivo and transactivated ABI5. Expression of ABI5, in which Ser-42 was mutated to alanine, could not fully rescue the ABA -insensitive phenotypes of the abi5-8 and pks5-4abi5-8 mutants. In contrast, mutating Ser-42 to aspartate rescued the ABA insensitivity of these mutants. These data demonstrate that PKS5-mediated phosphorylation of ABI5 at Ser-42 is critical for the ABA regulation of seed germination and gene expression in Arabidopsis ( Arabidopsis thaliana ).</abstract><pub>American Society of Plant Biologists</pub><pmid>25858916</pmid><doi>10.1104/pp.114.255455</doi></addata></record>
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title SOS2-LIKE PROTEIN KINASE5, an SNF1-RELATED PROTEIN KINASE3-Type Protein Kinase, Is Important for Abscisic Acid Responses in Arabidopsis through Phosphorylation of ABSCISIC ACID-INSENSITIVE51[OPEN]
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