Characterization of aminoglycoside resistance and virulence genes among Enterococcus spp. isolated from a hospital in China

This study investigated the aminoglycoside resistance phenotypes and genotypes, as well as the prevalence of virulence genes, in Enterococcus species isolated from clinical patients in China. A total of 160 enterococcal isolates from various clinical samples collected from September 2013 to July 201...

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Bibliographic Details
Published in:International journal of environmental research and public health 2015-03, Vol.12 (3), p.3014-3025
Main Authors: Li, Wanxiang, Li, Jing, Wei, Quhao, Hu, Qingfeng, Lin, Xiaowei, Chen, Mengquan, Ye, Renji, Lv, Huoyang
Format: Article
Language:English
Subjects:
Aminoglycosides - pharmacology
Anti-Bacterial Agents - pharmacology
Bacteria
China
Drug Resistance, Bacterial - genetics
Enterococcus
Enterococcus - drug effects
Enterococcus - genetics
Enterococcus - pathogenicity
Genes
Genes, Bacterial
Hospitals
Humans
Microbial Sensitivity Tests
Polymerase Chain Reaction
Studies
Virulence - genetics
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Summary:This study investigated the aminoglycoside resistance phenotypes and genotypes, as well as the prevalence of virulence genes, in Enterococcus species isolated from clinical patients in China. A total of 160 enterococcal isolates from various clinical samples collected from September 2013 to July 2014 were identified to the species level using the VITEK-2 COMPACT system. The antimicrobial susceptibilities of the identified Enterococcus strains were determined by the Kirby-Bauer (K-B) disc diffusion method. PCR-based assays were used to detect the aminoglycoside resistance and virulence genes in all enterococcal isolates. Of 160 Enterococcus isolates, 105 were identified as E. faecium, 35 as E. faecalis, and 20 isolates were classified as "other" Enterococcus species. High-level aminoglycoside resistance (HLAR) for gentamicin, streptomycin, and both antibiotics was identified in 58.8, 50, and 34.4% of strains, respectively. The most common virulence gene (50.6% of isolates) was efaA, followed by asa1 (28.8%). The most prevalent aminoglycoside resistance genes were aac(6')-Ie-aph(2''), aph(2')-Id, aph(3')-IIIa, and ant(6')-Ia, present in 49.4%, 1.3%, 48.8% and 31.3% of strains, respectively. Overall, E. faecium and E. faecalis were most frequently associated with hospital-acquired enterococcal infections in Zhejiang Province. All aminoglycoside resistance genes, except aph(2'')-Id, were significantly more prevalent in HLAR strains than amongst high level aminoglycoside susceptible (HLAS) strains, while there was no significant difference between HLAR and HLAS strains in regard to the prevalence of virulence genes, apart from esp, therefore, measures should be taken to manage infections caused by multi-drug resistant Enterococcus species.
ISSN:1660-4601
1661-7827
1660-4601
DOI:10.3390/ijerph120303014