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Ptychographic X-ray nanotomography quantifies mineral distributions in human dentine
Bones are bio-composites with biologically tunable mechanical properties, where a polymer matrix of nanofibrillar collagen is reinforced by apatite mineral crystals. Some bones, such as antler, form and change rapidly, while other bone tissues, such as human tooth dentine, develop slowly and maintai...
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Published in: | Scientific reports 2015-03, Vol.5 (1), p.9210, Article 9210 |
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description | Bones are bio-composites with biologically tunable mechanical properties, where a polymer matrix of nanofibrillar collagen is reinforced by apatite mineral crystals. Some bones, such as antler, form and change rapidly, while other bone tissues, such as human tooth dentine, develop slowly and maintain constant composition and architecture for entire lifetimes. When studying apatite mineral microarchitecture, mineral distributions or mineralization activity of bone-forming cells, representative samples of tissue are best studied at submicrometre resolution while minimizing sample-preparation damage. Here, we demonstrate the power of ptychographic X-ray tomography to map variations in the mineral content distribution in three dimensions and at the nanometre scale. Using this non-destructive method, we observe nanostructures surrounding hollow tracts that exist in human dentine forming dentinal tubules. We reveal unprecedented quantitative details of the ultrastructure clearly revealing the spatially varying mineralization density. Such information is essential for understanding a variety of natural and therapeutic effects for example in bone tissue healing and ageing. |
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Some bones, such as antler, form and change rapidly, while other bone tissues, such as human tooth dentine, develop slowly and maintain constant composition and architecture for entire lifetimes. When studying apatite mineral microarchitecture, mineral distributions or mineralization activity of bone-forming cells, representative samples of tissue are best studied at submicrometre resolution while minimizing sample-preparation damage. Here, we demonstrate the power of ptychographic X-ray tomography to map variations in the mineral content distribution in three dimensions and at the nanometre scale. Using this non-destructive method, we observe nanostructures surrounding hollow tracts that exist in human dentine forming dentinal tubules. We reveal unprecedented quantitative details of the ultrastructure clearly revealing the spatially varying mineralization density. Such information is essential for understanding a variety of natural and therapeutic effects for example in bone tissue healing and ageing.</description><identifier>ISSN: 2045-2322</identifier><identifier>EISSN: 2045-2322</identifier><identifier>DOI: 10.1038/srep09210</identifier><identifier>PMID: 25790969</identifier><language>eng</language><publisher>England: Nature Publishing Group</publisher><subject>Apatite ; Bone healing ; Bones ; Collagen ; Crystals ; Dentin - chemistry ; Humans ; Mechanical properties ; Microscopy, Electron ; Mineralization ; Minerals - analysis ; Teeth ; Tubules ; Ultrastructure ; X-Ray Microtomography - methods</subject><ispartof>Scientific reports, 2015-03, Vol.5 (1), p.9210, Article 9210</ispartof><rights>Copyright Nature Publishing Group Mar 2015</rights><rights>Copyright © 2015, Macmillan Publishers Limited. All rights reserved 2015 Macmillan Publishers Limited. All rights reserved</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c403t-aadefecbcbfc813d22712558932b904400a5d316ae24e1b25a166cd80a3589b83</citedby><cites>FETCH-LOGICAL-c403t-aadefecbcbfc813d22712558932b904400a5d316ae24e1b25a166cd80a3589b83</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.proquest.com/docview/1898633665/fulltextPDF?pq-origsite=primo$$EPDF$$P50$$Gproquest$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.proquest.com/docview/1898633665?pq-origsite=primo$$EHTML$$P50$$Gproquest$$Hfree_for_read</linktohtml><link.rule.ids>230,315,733,786,790,891,25783,27957,27958,37047,44625,53827,53829,75483</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/25790969$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Zanette, I</creatorcontrib><creatorcontrib>Enders, B</creatorcontrib><creatorcontrib>Dierolf, M</creatorcontrib><creatorcontrib>Thibault, P</creatorcontrib><creatorcontrib>Gradl, R</creatorcontrib><creatorcontrib>Diaz, A</creatorcontrib><creatorcontrib>Guizar-Sicairos, M</creatorcontrib><creatorcontrib>Menzel, A</creatorcontrib><creatorcontrib>Pfeiffer, F</creatorcontrib><creatorcontrib>Zaslansky, P</creatorcontrib><title>Ptychographic X-ray nanotomography quantifies mineral distributions in human dentine</title><title>Scientific reports</title><addtitle>Sci Rep</addtitle><description>Bones are bio-composites with biologically tunable mechanical properties, where a polymer matrix of nanofibrillar collagen is reinforced by apatite mineral crystals. 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subjects | Apatite Bone healing Bones Collagen Crystals Dentin - chemistry Humans Mechanical properties Microscopy, Electron Mineralization Minerals - analysis Teeth Tubules Ultrastructure X-Ray Microtomography - methods |
title | Ptychographic X-ray nanotomography quantifies mineral distributions in human dentine |
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