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STIM1 and STIM2 Proteins Differently Regulate Endogenous Store-operated Channels in HEK293 Cells
The endoplasmic reticulum calcium sensors stromal interaction molecules 1 and 2 (STIM1 and STIM2) are key modulators of store-operated calcium entry. Both these sensors play a major role in physiological functions in normal tissue and in pathology, but available data on native STIM2-regulated plasma...
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| Published in: | The Journal of biological chemistry 2015-02, Vol.290 (8), p.4717-4727 |
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| creator | Shalygin, Alexey Skopin, Anton Kalinina, Vera Zimina, Olga Glushankova, Lyuba Mozhayeva, Galina N. Kaznacheyeva, Elena |
| description | The endoplasmic reticulum calcium sensors stromal interaction molecules 1 and 2 (STIM1 and STIM2) are key modulators of store-operated calcium entry. Both these sensors play a major role in physiological functions in normal tissue and in pathology, but available data on native STIM2-regulated plasma membrane channels are scarce. Only a few studies have recorded STIM2-induced CRAC (calcium release-activated calcium) currents. On the other hand, many cell types display store-operated currents different from CRAC. The STIM1 protein regulates not only CRAC but also transient receptor potential canonical (TRPC) channels, but it has remained unclear whether STIM2 is capable of regulating store-operated non-CRAC channels. Here we present for the first time experimental evidence for the existence of endogenous non-CRAC STIM2-regulated channels. As shown in single-channel patch clamp experiments on HEK293 cells, selective activation of native STIM2 proteins or STIM2 overexpression results in store-operated activation of Imin channels, whereas STIM1 activation blocks this process. Changes in the ratio between active STIM2 and STIM1 proteins can switch the regulation of Imin channels between store-operated and store-independent modes. We have previously characterized electrophysiological properties of different Ca2+ influx channels coexisting in HEK293 cells. The results of this study show that STIM1 and STIM2 differ in the ability to activate these store-operated channels; Imin channels are regulated by STIM2, TRPC3-containing INS channels are induced by STIM1, and TRPC1-composed Imax channels are activated by both STIM1 and STIM2. These new data about cross-talk between STIM1 and STIM2 and their different roles in store-operated channel activation are indicative of an additional level in the regulation of store-operated calcium entry pathways.
Background: STIM calcium sensors are key modulators of store-operated channels (SOCs).
Results: Changes in the ratio of active STIM2/STIM1 switch Imin channel regulation between store-operated and store-independent modes.
Conclusion: Endogenous SOCs are differently regulated by STIM1 and STIM2.
Significance: Cross-talk between STIM1 and STIM2 and their different roles in channel activation are indicative of an additional level of SOC regulation. |
| doi_str_mv | 10.1074/jbc.M114.601856 |
| format | article |
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Background: STIM calcium sensors are key modulators of store-operated channels (SOCs).
Results: Changes in the ratio of active STIM2/STIM1 switch Imin channel regulation between store-operated and store-independent modes.
Conclusion: Endogenous SOCs are differently regulated by STIM1 and STIM2.
Significance: Cross-talk between STIM1 and STIM2 and their different roles in channel activation are indicative of an additional level of SOC regulation.</description><identifier>ISSN: 0021-9258</identifier><identifier>EISSN: 1083-351X</identifier><identifier>DOI: 10.1074/jbc.M114.601856</identifier><identifier>PMID: 25533457</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Calcium ; Calcium - metabolism ; Calcium Channel ; Calcium Signaling - physiology ; Cell Adhesion Molecules - genetics ; Cell Adhesion Molecules - metabolism ; Cell Biology ; Endoplasmic Reticulum (ER) ; HEK293 ; HEK293 Cells ; Humans ; Imin ; Membrane Proteins - genetics ; Membrane Proteins - metabolism ; Neoplasm Proteins - genetics ; Neoplasm Proteins - metabolism ; Patch Clamp ; Single Channel ; SOC ; STIM2 ; Stromal Interaction Molecule 1 ; Stromal Interaction Molecule 1 (STIM1) ; Stromal Interaction Molecule 2 ; Stromal Interaction Molecule 2 (STIM2) ; TRPC Cation Channels - genetics ; TRPC Cation Channels - metabolism</subject><ispartof>The Journal of biological chemistry, 2015-02, Vol.290 (8), p.4717-4727</ispartof><rights>2015 © 2015 ASBMB. Currently published by Elsevier Inc; originally published by American Society for Biochemistry and Molecular Biology.</rights><rights>2015 by The American Society for Biochemistry and Molecular Biology, Inc.</rights><rights>2015 by The American Society for Biochemistry and Molecular Biology, Inc. 2015</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c443t-471aa5a6c2383f96ee33caca8131a1cd60df03405a0e7394dccefd71bbdaafbb3</citedby><cites>FETCH-LOGICAL-c443t-471aa5a6c2383f96ee33caca8131a1cd60df03405a0e7394dccefd71bbdaafbb3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4335210/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4335210/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,315,733,786,790,891,27957,27958,53827,53829</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/25533457$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Shalygin, Alexey</creatorcontrib><creatorcontrib>Skopin, Anton</creatorcontrib><creatorcontrib>Kalinina, Vera</creatorcontrib><creatorcontrib>Zimina, Olga</creatorcontrib><creatorcontrib>Glushankova, Lyuba</creatorcontrib><creatorcontrib>Mozhayeva, Galina N.</creatorcontrib><creatorcontrib>Kaznacheyeva, Elena</creatorcontrib><title>STIM1 and STIM2 Proteins Differently Regulate Endogenous Store-operated Channels in HEK293 Cells</title><title>The Journal of biological chemistry</title><addtitle>J Biol Chem</addtitle><description>The endoplasmic reticulum calcium sensors stromal interaction molecules 1 and 2 (STIM1 and STIM2) are key modulators of store-operated calcium entry. Both these sensors play a major role in physiological functions in normal tissue and in pathology, but available data on native STIM2-regulated plasma membrane channels are scarce. Only a few studies have recorded STIM2-induced CRAC (calcium release-activated calcium) currents. On the other hand, many cell types display store-operated currents different from CRAC. The STIM1 protein regulates not only CRAC but also transient receptor potential canonical (TRPC) channels, but it has remained unclear whether STIM2 is capable of regulating store-operated non-CRAC channels. Here we present for the first time experimental evidence for the existence of endogenous non-CRAC STIM2-regulated channels. As shown in single-channel patch clamp experiments on HEK293 cells, selective activation of native STIM2 proteins or STIM2 overexpression results in store-operated activation of Imin channels, whereas STIM1 activation blocks this process. Changes in the ratio between active STIM2 and STIM1 proteins can switch the regulation of Imin channels between store-operated and store-independent modes. We have previously characterized electrophysiological properties of different Ca2+ influx channels coexisting in HEK293 cells. The results of this study show that STIM1 and STIM2 differ in the ability to activate these store-operated channels; Imin channels are regulated by STIM2, TRPC3-containing INS channels are induced by STIM1, and TRPC1-composed Imax channels are activated by both STIM1 and STIM2. These new data about cross-talk between STIM1 and STIM2 and their different roles in store-operated channel activation are indicative of an additional level in the regulation of store-operated calcium entry pathways.
Background: STIM calcium sensors are key modulators of store-operated channels (SOCs).
Results: Changes in the ratio of active STIM2/STIM1 switch Imin channel regulation between store-operated and store-independent modes.
Conclusion: Endogenous SOCs are differently regulated by STIM1 and STIM2.
Significance: Cross-talk between STIM1 and STIM2 and their different roles in channel activation are indicative of an additional level of SOC regulation.</description><subject>Calcium</subject><subject>Calcium - metabolism</subject><subject>Calcium Channel</subject><subject>Calcium Signaling - physiology</subject><subject>Cell Adhesion Molecules - genetics</subject><subject>Cell Adhesion Molecules - metabolism</subject><subject>Cell Biology</subject><subject>Endoplasmic Reticulum (ER)</subject><subject>HEK293</subject><subject>HEK293 Cells</subject><subject>Humans</subject><subject>Imin</subject><subject>Membrane Proteins - genetics</subject><subject>Membrane Proteins - metabolism</subject><subject>Neoplasm Proteins - genetics</subject><subject>Neoplasm Proteins - metabolism</subject><subject>Patch Clamp</subject><subject>Single Channel</subject><subject>SOC</subject><subject>STIM2</subject><subject>Stromal Interaction Molecule 1</subject><subject>Stromal Interaction Molecule 1 (STIM1)</subject><subject>Stromal Interaction Molecule 2</subject><subject>Stromal Interaction Molecule 2 (STIM2)</subject><subject>TRPC Cation Channels - genetics</subject><subject>TRPC Cation Channels - metabolism</subject><issn>0021-9258</issn><issn>1083-351X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><recordid>eNp1kc9PHCEUx0lTU1fbs7eGYy-z8gaYH5cmzXb9ETUatUlvyMCbFTMLW5g18b-XzaqxB7lA8j58eY8PIQfApsBqcfjQmekFgJhWDBpZfSITYA0vuIS_n8mEsRKKtpTNLtlL6YHlJVr4QnZLKTkXsp6Qu5vb0wug2lu6OZX0KoYRnU_0t-t7jOjH4Yle42I96BHp3NuwQB_Wid6MIWIRVhhzwdLZvfYeh0Sdpyfzs7LldIbDkL6SnV4PCb-97Pvkz9H8dnZSnF8en85-nRdGCD4Wogatpa5MyRvetxUi50Yb3QAHDcZWzPaMCyY1w5q3whqDva2h66zWfdfxffJzm7tad0u0Jjce9aBW0S11fFJBO_V_xbt7tQiPSnAuS2A54MdLQAz_1phGtXTJ5BG0xzyvgko2NZOZzOjhFjUxpBSxf3sGmNp4UdmL2nhRWy_5xvf33b3xryIy0G6B_IX46DCqZBx6g9ZFNKOywX0Y_gy_iZ1_</recordid><startdate>20150220</startdate><enddate>20150220</enddate><creator>Shalygin, Alexey</creator><creator>Skopin, Anton</creator><creator>Kalinina, Vera</creator><creator>Zimina, Olga</creator><creator>Glushankova, Lyuba</creator><creator>Mozhayeva, Galina N.</creator><creator>Kaznacheyeva, Elena</creator><general>Elsevier Inc</general><general>American Society for Biochemistry and Molecular Biology</general><scope>6I.</scope><scope>AAFTH</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20150220</creationdate><title>STIM1 and STIM2 Proteins Differently Regulate Endogenous Store-operated Channels in HEK293 Cells</title><author>Shalygin, Alexey ; Skopin, Anton ; Kalinina, Vera ; Zimina, Olga ; Glushankova, Lyuba ; Mozhayeva, Galina N. ; Kaznacheyeva, Elena</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c443t-471aa5a6c2383f96ee33caca8131a1cd60df03405a0e7394dccefd71bbdaafbb3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2015</creationdate><topic>Calcium</topic><topic>Calcium - metabolism</topic><topic>Calcium Channel</topic><topic>Calcium Signaling - physiology</topic><topic>Cell Adhesion Molecules - genetics</topic><topic>Cell Adhesion Molecules - metabolism</topic><topic>Cell Biology</topic><topic>Endoplasmic Reticulum (ER)</topic><topic>HEK293</topic><topic>HEK293 Cells</topic><topic>Humans</topic><topic>Imin</topic><topic>Membrane Proteins - genetics</topic><topic>Membrane Proteins - metabolism</topic><topic>Neoplasm Proteins - genetics</topic><topic>Neoplasm Proteins - metabolism</topic><topic>Patch Clamp</topic><topic>Single Channel</topic><topic>SOC</topic><topic>STIM2</topic><topic>Stromal Interaction Molecule 1</topic><topic>Stromal Interaction Molecule 1 (STIM1)</topic><topic>Stromal Interaction Molecule 2</topic><topic>Stromal Interaction Molecule 2 (STIM2)</topic><topic>TRPC Cation Channels - genetics</topic><topic>TRPC Cation Channels - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Shalygin, Alexey</creatorcontrib><creatorcontrib>Skopin, Anton</creatorcontrib><creatorcontrib>Kalinina, Vera</creatorcontrib><creatorcontrib>Zimina, Olga</creatorcontrib><creatorcontrib>Glushankova, Lyuba</creatorcontrib><creatorcontrib>Mozhayeva, Galina N.</creatorcontrib><creatorcontrib>Kaznacheyeva, Elena</creatorcontrib><collection>ScienceDirect Open Access Titles</collection><collection>Elsevier:ScienceDirect:Open Access</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>The Journal of biological chemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Shalygin, Alexey</au><au>Skopin, Anton</au><au>Kalinina, Vera</au><au>Zimina, Olga</au><au>Glushankova, Lyuba</au><au>Mozhayeva, Galina N.</au><au>Kaznacheyeva, Elena</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>STIM1 and STIM2 Proteins Differently Regulate Endogenous Store-operated Channels in HEK293 Cells</atitle><jtitle>The Journal of biological chemistry</jtitle><addtitle>J Biol Chem</addtitle><date>2015-02-20</date><risdate>2015</risdate><volume>290</volume><issue>8</issue><spage>4717</spage><epage>4727</epage><pages>4717-4727</pages><issn>0021-9258</issn><eissn>1083-351X</eissn><notes>ObjectType-Article-1</notes><notes>SourceType-Scholarly Journals-1</notes><notes>ObjectType-Feature-2</notes><notes>content type line 23</notes><abstract>The endoplasmic reticulum calcium sensors stromal interaction molecules 1 and 2 (STIM1 and STIM2) are key modulators of store-operated calcium entry. Both these sensors play a major role in physiological functions in normal tissue and in pathology, but available data on native STIM2-regulated plasma membrane channels are scarce. Only a few studies have recorded STIM2-induced CRAC (calcium release-activated calcium) currents. On the other hand, many cell types display store-operated currents different from CRAC. The STIM1 protein regulates not only CRAC but also transient receptor potential canonical (TRPC) channels, but it has remained unclear whether STIM2 is capable of regulating store-operated non-CRAC channels. Here we present for the first time experimental evidence for the existence of endogenous non-CRAC STIM2-regulated channels. As shown in single-channel patch clamp experiments on HEK293 cells, selective activation of native STIM2 proteins or STIM2 overexpression results in store-operated activation of Imin channels, whereas STIM1 activation blocks this process. Changes in the ratio between active STIM2 and STIM1 proteins can switch the regulation of Imin channels between store-operated and store-independent modes. We have previously characterized electrophysiological properties of different Ca2+ influx channels coexisting in HEK293 cells. The results of this study show that STIM1 and STIM2 differ in the ability to activate these store-operated channels; Imin channels are regulated by STIM2, TRPC3-containing INS channels are induced by STIM1, and TRPC1-composed Imax channels are activated by both STIM1 and STIM2. These new data about cross-talk between STIM1 and STIM2 and their different roles in store-operated channel activation are indicative of an additional level in the regulation of store-operated calcium entry pathways.
Background: STIM calcium sensors are key modulators of store-operated channels (SOCs).
Results: Changes in the ratio of active STIM2/STIM1 switch Imin channel regulation between store-operated and store-independent modes.
Conclusion: Endogenous SOCs are differently regulated by STIM1 and STIM2.
Significance: Cross-talk between STIM1 and STIM2 and their different roles in channel activation are indicative of an additional level of SOC regulation.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>25533457</pmid><doi>10.1074/jbc.M114.601856</doi><tpages>11</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | The Journal of biological chemistry, 2015-02, Vol.290 (8), p.4717-4727 |
| issn | 0021-9258 1083-351X |
| language | eng |
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| source | BACON - Elsevier - GLOBAL_SCIENCEDIRECT-OPENACCESS; PubMed Central |
| subjects | Calcium Calcium - metabolism Calcium Channel Calcium Signaling - physiology Cell Adhesion Molecules - genetics Cell Adhesion Molecules - metabolism Cell Biology Endoplasmic Reticulum (ER) HEK293 HEK293 Cells Humans Imin Membrane Proteins - genetics Membrane Proteins - metabolism Neoplasm Proteins - genetics Neoplasm Proteins - metabolism Patch Clamp Single Channel SOC STIM2 Stromal Interaction Molecule 1 Stromal Interaction Molecule 1 (STIM1) Stromal Interaction Molecule 2 Stromal Interaction Molecule 2 (STIM2) TRPC Cation Channels - genetics TRPC Cation Channels - metabolism |
| title | STIM1 and STIM2 Proteins Differently Regulate Endogenous Store-operated Channels in HEK293 Cells |
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