Glucocorticoids increase osmotic water permeability (Pf) of neonatal rabbit renal brush border membrane vesicles

Departments of 1 Pediatrics and 2 Internal Medicine, University of Texas Southwestern Medical Center at Dallas, Dallas, Texas Submitted 7 July 2004 ; accepted in final form 13 January 2005 During postnatal maturation, there is an increase in renal brush border membrane vesicle (BBMV) osmotic water p...

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Published in:American journal of physiology. Regulatory, integrative and comparative physiology integrative and comparative physiology, 2005-05, Vol.288 (5), p.R1417-R1421
Main Authors: Mulder, Jaap, Chakravarty, Sumana, Haddad, Maha N, Baum, Michel, Quigley, Raymond
Format: Article
Language:English
Subjects:
Aging - metabolism
Aging - physiology
Animals
Animals, Newborn - metabolism
Animals, Newborn - physiology
Aquaporin 1
Aquaporins - biosynthesis
Dexamethasone - pharmacology
Glucocorticoids - pharmacology
Glucocorticoids - physiology
Kidney Cortex - drug effects
Kidney Cortex - metabolism
Kidney Cortex - physiology
Kidney Tubules, Proximal - drug effects
Kidney Tubules, Proximal - metabolism
Kidney Tubules, Proximal - physiology
Microvilli - drug effects
Microvilli - metabolism
Microvilli - physiology
Permeability - drug effects
Rabbits
Water - metabolism
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Summary:Departments of 1 Pediatrics and 2 Internal Medicine, University of Texas Southwestern Medical Center at Dallas, Dallas, Texas Submitted 7 July 2004 ; accepted in final form 13 January 2005 During postnatal maturation, there is an increase in renal brush border membrane vesicle (BBMV) osmotic water permeability and a parallel increase in aquaporin-1 (AQP1) protein abundance. The mechanisms responsible for these changes remain unknown. Because serum glucocorticoid levels rise postnatally and have previously been linked to other maturational changes in renal function, we examined the effects of glucocorticoids on osmotic (P f ) and diffusional (P DW ) water permeability and AQP1 protein abundance of renal BBMV. Neonatal rabbits were treated with dexamethasone (10 µg/100 g) for three days and compared with control neonates and adults. P f and P DW were measured at 20°C with a stopped-flow apparatus using light-scattering and aminonaphthalene trisulfonic acid (ANTS) fluorescence, respectively. P f was significantly higher in BBMV from dexamethasone-treated neonates compared with vehicle-treated neonates, but remained lower than in BBMV from adults ( P < 0.05). P DW in dexamethasone and vehicle-treated neonatal BBMV was lower than in adult BBMV. P f /P DW ratio increased from neonate (5.1 ± 0.3) to dexamethasone (7.0 ± 0.1) and adult BBMV (6.3 ± 0.1). AQP1 expression was increased by dexamethasone treatment to adult levels. Membrane fluidity, which is inversely related to generalized polarization (GP) of steady-state laurdan fluorescence, was significantly higher in neonatal BBMV than both dexamethasone and adult BBMV (GP: neonate 0.285 ± 0.002, dexamethasone treatment 0.302 ± 0.006, and adult 0.300 ± 0.005; P < 0.05). These combined results show that dexamethasone-treatment during days 4–7 of life increases BBMV water permeability despite a decrease in membrane fluidity. This occurs by increasing channel-mediated water transport, as reflected in an increase in AQP1 protein abundance and a higher P f /P DW ratio. This mimics the maturational changes and suggests a physiological role for glucocorticoids in maturation of proximal tubule water transport. stop-flow kinetics; diffusional water permeability; development; aquaporin Address for reprint requests and other correspondence: Raymond Quigley, Dept. of Pediatrics, U. T. Southwestern Medical Center, 5323 Harry Hines Blvd., Dallas, TX 75390–9063 (E-mail: raymond.quigley{at}utsouthwestern.edu )
ISSN:0363-6119
1522-1490
DOI:10.1152/ajpregu.00448.2004